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Abstracts fied 19.2% (24/125) more positive cases, 50% (12/24) were HPV18, 37.5% (9/24) HPV16 and in 12.5% (3/24) amplification of more than one genotype occurred. Conclusion: In conclusion, target region for amplification of HPV DNA is a determinant factor in the molecular diagnosis of cervical infection. Moreover, quantification of the viral load by qRT-PCR SYBR Green-L1 assay allows to determines HPV infection, viral load, genotyping especially in women with high risks lesions by PAP smear.doi:10.1016/j.ijid.2010.02.675 fatcat:5bdwgnjcjzaydl4jsb33427ll4