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The strain of Drosophila auraria used in the present study collected by putting rotten fruit baits was cultured on ripe banana media. The life cycle is approximately 12 days from egg to egg at 28°C. Third instar larvae were selected to dissect for salivary gland chromosomes, after 2% acetolactic orcein for 15 minutes cover slip pressed, a giant chromosome map was prepared by measuring: the widths and lengths of the bands and puffs working from free end to centromeric end. The salivary glandfatcat:foijpkka3jdpxoy2ven4tepq7m