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Designed hybrids facilitate efficient generation of high-resolution linkage maps [article]

Kazutoshi Yoshitake, Yoji Igarashi, Misaki Mizukoshi, Shigeharu Kinoshita, Susumu Mitsuyama, Yutaka Suzuki, Kazuyoshi Saito, Shugo Watabe, Shuichi Asakawa
2018 bioRxiv   pre-print
In sequencing eukaryotic genomes, linkage maps are indispensable for building scaffolds with which to assemble and/or to validate chromosomes. However, current approaches to construct linkage maps are limited by marker density and cost-effectiveness, especially for wild organisms. We have now devised a new strategy based on artificially generated hybrid organisms to acquire ultra high-density genomic markers at lower cost and build highly accurate linkage maps. Using this method, linkage maps
more » ... hod, linkage maps and draft sequences for two species of pufferfish were obtained simultaneously. We anticipate that the method will accelerate genomic analysis of sexually reproducing organisms.
doi:10.1101/243451 fatcat:e3w2xasltzf5hecjsnfdncpism