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Chemically bonded solid phase materials are very important in separation chemistry. The chemically modified silica gel contains rest silanol groups that can affect the retention properties when they are used for separation of compounds. Some commercially available solid phase extraction (SPE) materials based on silica gel were analysed for rest silanol groups by near infrared spectroscopy. The combination frequency of the water molecules in the 5500- 5000 cm -1 region was used in identifyingdoi:10.4028/www.scientific.net/amr.650.66 fatcat:r3b2nexji5fefnap2vswvkut3y