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International Journal of current research in pharmacy
Every day many chromatographers face the need to develop a high-performance liquid chromatography (HPLC) method. This review describes systematic procedures for developing an HPLC method, based on the best information available. HPLC method development involves several essential steps: sample pretreatment, detection of sample bands, choosing separation conditions, quantitation, and method validation. HPLC is an analytical tool which is able to detect, separate and quantify the drug, its variousfatcat:u4pxlmcxbfhg3nz5qkbpbpwdum