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Review Article Development and validation of HPLC method-A Review Corresponding Author

Vijendra Rajan, Mr Rajan
2015 International Journal of current research in pharmacy   unpublished
Every day many chromatographers face the need to develop a high-performance liquid chromatography (HPLC) method. This review describes systematic procedures for developing an HPLC method, based on the best information available. HPLC method development involves several essential steps: sample pretreatment, detection of sample bands, choosing separation conditions, quantitation, and method validation. HPLC is an analytical tool which is able to detect, separate and quantify the drug, its various
more » ... e drug, its various impurities and drug related degrades that can form on synthesis or storage. It also includes the understanding of chemistry of drug substance and improves the development of analytical method. Validation is to performed to determine the limits of allowed variability for the conditions needed to run the method. This article describes strategies & an issue comes in development of a HPLC method development & validation.