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A consistent correlation between elevated esterase activity and methyl parathion resistance among Nebraska western corn rootworm, Diabrotica virgifera virgifera LeConte, populations has previously been documented. Characterization of general esterase activity using naphtholic esters as model substrates indicated that differences between resistant and susceptible strains could be maximized by optimizing assay conditions. The optimal conditions identiÞed here were similar to those reported fordoi:10.1603/0022-0493-96.6.1855 pmid:14977127 fatcat:b6qqhd3d6nfv5pe74z6nc6iigy