Non-invasive method to detect the changes of glucose concentration in whole blood using photometric technique
2014 36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society
A noninvasive optical method is developed to monitor rapid changes in blood glucose levels in diabetic patients. The system depends on an optical cell built with a LED that emits light of wavelength 535nm that is a peak absorbance of hemoglobin. As the glucose concentration in the blood decreases, its osmolarity also decreases and the RBCs swell and decrease the path length absorption coefficient. Decreasing absorption coefficient increases the transmission of light through the whole blood. The
... he whole blood. The system was tested with a constructed optical cell that held whole blood in a capillary tube. As expected the light transmitted to the photodiode increases with decreasing glucose concentration. The average response time of the system was between 30-40 seconds. The changes in size of the RBC cells in response to glucose concentration changes were confirmed using a cell counter and also visually under microscope. This method does not allow measuring the glucose concentration with an absolute concentration calibration. It is directed towards development of a device to monitor the changes in glucose concentration as an aid to diabetic management. This method might be improvised for precision and resolution and be developed as a ring or an earring that patients can wear. ii DEDICATION This is dedicated to my parents, sister, relatives and all my friends who were always supportive. (Dad and mom, I did it!) iii ACKNOWLEDGMENTS I would like to thank Dr. Bruce Towe, committee chair for his impeccable support, guidance and patience in helping me complete this thesis. I also would like to thank Dr. Jeffrey LaBelle and Dr. Jitendran Muthuswamy for their support. Without the help of Daniel Gulick I would not have found any components in the lab and worked with ease. I would like to thank Dan and other lab mates for their patience and assistance that they provided throughout my time in the lab. I would also extend my gratitude to Mark Richards and Dmitry Derkach for their assistance with 3D printing, cell counter and their questions that molded my project work.