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Analysis and stability test of the extracts from Ulmus parvifolia leaves

Doo-Young Kim, Soobin Song, Iljoo Kim, Se-Kyoo Jeong, Sungwoo Kim, Jung-Hee Kim, Hyun-Jae Jang, Sei-Ryang Oh, Hyung Won Ryu
2020 Journal of Applied Biological Chemistry  
A simple and reliable HPLC method was developed to determine pharmacologically standard marker compounds of Ulmus parvifolia leaves. Standard markers were characterized with neochlorogenic acid (trans-5-O-caffeoylquinic acid, 5-CQA) and chlorogenic acid (trans-3-O-caffeoylquinic acid, 3-CQA) using NMR and UPLC-QTof-MS analysis. A method for qualitative/ quantitative analysis of the leaves extracts were evaluated including two compounds by using HPLC. The stability test of 30% ethanolic extracts
more » ... ethanolic extracts of the leaves sample and standard markers have been evaluated for six months. However, no significant changes in the content of the marker compounds of each extract was observed during the time of investigation.
doi:10.3839/jabc.2020.053 fatcat:rpkclppo3nbzxhxturybsexxrm