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The Effect of the Aqueous Enzymatic Extraction Method towards Momordica charantia Seed Oil and Its Lignocellulosic Biomass

Nadirah Zawani MOHD NESFU, Hasnah OSMAN, Suriyati MOHAMAD, Lionel MUNIGLIA, Nicolas BROSSE, Dominique LAURAIN-MATTAR, Rosella SPINA, Ezatul E. KAMARULZAMAN
2021 Walailak Journal of Science and Technology  
The development of a sustainable product using natural resources will gain much attention nowadays. This work aimed to study the effect of aqueous enzymatic extraction (AEE) using a different ratio of enzyme cocktails HEL1 and X7 towards the production of Momordica charantia L. (M. charantia) seed oil and to identify the composition of the seeds' lignocellulosic biomass (extracted-free materials, EFM). The M. charantia seed oil contained omega-3 fatty acids, such as stearidonic acid, that
more » ... ic acid, that possess hypotriglyceridemic properties. The oil obtained was derivatized to fatty acid methyl esters (FAMEs) before biochemical quantification using gas chromatography (GC). The EFM was subjected to Soxhlet extraction and further analyzed to identify lignin, acid-soluble sugars, and hemicellulose composition. The results showed that the highest seed oil percentage, (6.26±0.53 %) was extracted from M2 sample using a combination of 5 % HEL1 to 1.25 % X7 enzyme cocktails, as compared to the extraction without enzyme (3.32±0.38 %). The percentage of stearidonic acid in the seed oil increased from 13.55 % (without enzyme) to 19.43 % (M2), proving that there were some change in terms of fatty acid composition in the seed oil.
doi:10.48048/wjst.2021.9098 fatcat:slfevtfgcre7visap6yh3m4jry