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Estudo comparativo de membrana serosa porcina e de elastina no reparo de defeitos na calvária de ratos [thesis]

Fernando Bento Cunha
Palavras-chave: Elastina. Colágeno. Hidroxiapatita. Proteína morfogenética óssea. Regeneração óssea. ABSTRACT CUNHA, F.B. Comparative study of porcine serous membrane and elastin in the repair of defects in calvaria of rats. 2018. 117 f. Dissertation (Master in Sciences) -São Introduction: Pathologies that cause extensive bone loss still use autologous bone graft as the gold standard treatment. However, due to quantitative limitation, the use of elastin biomaterials and porcine intestinal
more » ... ne intestinal serosa collagen with hydroxyapatite, as well as the association with bone morphogenetic protein in regenerative bone tissue therapies are presented as alternatives. Objectives: To evaluate the osteogenic potential of natural polymer matrices based on elastin derived from bovine auricular cartilage and porcine gut serosa collagen, used alone or in combination with hydroxyapatite and bone morphogenetic protein, in the repair of bone defects in the skull of rats. Materials and Methods: 63 male rats (Rattus norvegicus, Wistar) weighing approximately 360 grams and 16 weeks of age were used. The animals were submitted to the surgical procedure to create the bone defect in the left parietal bone and were divided into 7 groups with 9 animals each. The groups were implanted with the following biomaterials: group 1 control (G1-C) without implant, group 2 (G2-E24 / 37) elastin membrane treated for 24h at 37 ° C, group 3 (G3-E24 / 37 / HA) elastin membrane treated for 24h at 37 ° C and hydroxyapatite, group 4 (G4-E24 / 37 / BMP) elastin membrane treated 24 h at 37 ° C and bone morphogenetic protein, group 5 (G5-SIP24/25) porcine intestinal serosa collagen membrane treated for 24h at 25° C, group 6 (G6-SIP24/25/ HA) porcine intestinal serosa collagen membrane treated for 24h at 25° C and hydroxyapatite, group 7 (G7-SIP24/25 / BMP) porcine intestinal serosa collagen membrane treated for 24h at 25° C and bone morphogenetic protein. After painless death induced in 6 weeks, the anatomical parts of the skull caps were removed for macroscopic, radiological, histological and morphometric analysis. Results: Macroscopic, radiographic and histological analyzes demonstrated the biocompatibility of the biomaterials used. The mean and standard deviations of the relative percentage volume of newly formed bone in the cranial defect of the control group (G1), the grafted groups with the elastin membrane 24h / 37 ° C (G2 to G4) and the grafted groups with the porcine intestinal serosa collagen membrane 24h / 25 ° C (G5 to G7) were: 5,86 ± 2,87; 13,68 ± 1,44; 17,65 ± 1,85; 29,46 ± 3.21; 53,20 ± 4,47; 30,45 ± 4,12; 38,47 ± 5,32, respectively Conclusion: Biomaterials were biocompatible and group 5 (G5-SIP24/25) showed the greatest bone neoformation.
doi:10.11606/d.82.2020.tde-27022020-142758 fatcat:jz2l7oh77zhx5k2ceecvad2j7y