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Cholangiocarcinoma (CC) is a rapidly lethal malignancy and currently is considered to be incurable. Biomarkers related to the development of CC remain unclear. The present study aimed to identify differentially expressed genes (DEGs) between normal tissue and intrahepatic CC, as well as specific gene expression patterns that changed together with the development of CC. By using a two-way analysis of variance test, the biomarkers that could distinguish between normal tissue and intrahepatic CCdoi:10.3892/mmr.2015.3216 pmid:25606976 fatcat:5kvy53uzdjesnlrqryltp4pblu