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Purificação e caracterização da quitinase de uva (Vitis vinífera L. cv Red Globe) para a produção de quitosana a partir de quitina de camarão

Laidson Paes Gomes, Carlos Ivan Ribeiro de Oliveira, Márcia Cristina da Silva, Cristina Tristão de Andrade, Eduardo Mere Del Aguila, Joab Trajano Silva, Vânia M. Flosi Paschoalin
2010 Química Nova  
Chitinase is produced by a wide variety of plants as a defense against peste attacks. In this study, grape chitinases were purified 16 times by fractionation in 80% ammonium sulfate followed by dialysis and filtration. Purified chitinases exhibited enzymatic activity toward chitin azure. The yield of purified chitinase was 229 mg/L with chitinase activity of 563 U/g. Chitinases had molecular masses of 24 and 30 kDa, as evaluated by SDS-PAGE 12.5%. Two pH optima were determined 3.0 and 6.0. The
more » ... d 3.0 and 6.0. The optimal temperature was 42 o C. Pre hydrolysis of crystalline shrimp chitin by chitinases caused in an increase in the deacetylation ratio triggered by chitin deacetylase producing chitooligosaccharides with DA (degree acetylation) of 58.8%.
doi:10.1590/s0100-40422010000900012 fatcat:jicxtpsignhczoqk7sqpoa5xzu