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The present study provides an efficient cryopreservation protocol for Tulipa tarda cultured in vitro. Apices were excised from bulblets cultivated on MS medium, supplemented with 60 or 90 g l − 1 sucrose. Half of the bulblets were subjected to a cold treatment at 5 °C for 10 weeks, before exposure of the apices to loading solution (LS) and plant vitrification solution 2 (PVS2). Ten weeks after rewarming and culture on recovery medium, 100% regrowth rates were obtained for cold treated explantsdoi:10.1007/s11240-020-01910-6 fatcat:oazmjl34vbatzkqnr3qcudyvbu