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PURPOSE To determine the long-term fate of donor epithelial cells after limbal allograft transplantation. METHODS. Corneal buttons and peripheral blood leukocytes were obtained at the time of penetrating keratoplasty from three patients who had undergone a successful limbal allograft transplantation. Microdissection was used to remove the corneal epithelial cells from the button. The presence of donor and recipient epithelial cells in each sample was determined by using PCR for DNAdoi:10.1167/iovs.04-0575 pmid:15728534 fatcat:moi6sfhcafhp3jsbxaaochc44a