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Three-Dimensional Culture of Feline Articular Chondrocytes in Alginate Microspheres

Hiroaki KAMISHINA, Takayoshi MIYABAYASHI, Roger M. CLEMMONS, James P. FARESE, Elizabeth W. UHL
2006 Journal of Veterinary Medical Science  
Chondrocytes isolated from proximal femoral articular cartilage from 3 adult cat cadavers were expanded in monolayer culture and subsequently cultured in alginate microspheres for 24 days. Cell proliferation and production of proteoglycans in alginate microspheres were observed during day 18 and 24. Quantification of chondroitin sulfates (CS) by capillary electrophoresis revealed that cultured chondrocytes synthesized CS6 but not CS4. Three-dimensional culture using alginate microspheres is a
more » ... microspheres is a useful in vitro technique to study proliferation and metabolism of chondrocytes; however, further modifications are needed to apply the technique to feline articular chondrocytes. KEY WORDS: alginate microsphere, articular chondrocyte, chondroitin sulfate.
doi:10.1292/jvms.68.1239 pmid:17146189 fatcat:w26iby6krbcn5kkehy55z2xmzq