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Change of Antioxidative Activity at Different Harvest Time and Improvement of Atopic Dermatitis Effects for Persimmon Leaf Extract
감잎(柿葉) 추출물의 채취시기에 따른 항산화 활성 변화 및 아토피성 피부염 개선효과

Woo-Young Jung, Jong-Moon Jeong
2012 The Korea Journal of Herbology  
Objectives:The purpose of this study was to investigate the anti-oxidative and anti-atopic dermatitis effects of persimmon leaf extract obtained from Cheongdo-gun, where more than 60% of Korean persimmon is produced. Methods:Anti-oxidative effects of the crude persimmon leaf extract harvested monthly between May and November were determined by in vitro assay using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and superoxide dismutase (SOD)-like reaction. Anti-atopic dermatitis effects of the crude
more » ... of the crude persimmon leaf extract were determined by using collagenase type Ⅰ inhibition assay and by quantitative assays including serum histamine, prostaglandin E metabolite and leukotriene B 4 levels in animal model of atopic dermatitis using Balb/c mice. Results:Persimmon leaf extract harvested in May had higher levels of total phenolic compounds (182.24 mg/g) and flavonoids (23.05 mg/g) than the ones of different month extract. Also, persimmon leaf extract harvested in May showed the most effective extract scavenging activities of DPPH free radical (13.39±0.21 µg/ml) and superoxide anion radical (40.52±2.32 µg/ml), leading to use the persimmon leaf extract harvested in May for the experiments hereafter. Persimmon leaf extract showed 326.71±4.6 µg/ml of 50% inhibitory concentration (IC 50 ) for collagenase type Ⅰ which is responsible for the degradation of extracellular matrix. In addition, persimmon leaf extract application group could decrease serum levels of histamine, prostaglandin E metabolite and leukotriene B 4 compared to the negative control in animal model of atopic dermatitis. Especially, persimmon leaf extract showed a significantly decreased serum leukotriene B 4 level relative to the levels of histamine and prostaglandin E metabolite. Conclusions:Persimmon leaf extract showed anti-oxidative and anti-atopic dermatitis effects in vitro and in vivo. These results suggest that persimmon leaf extract may have immunoregulatory function for alleviating atopic dermatitis by decreasing collagenase activity and mast cell activation. Key words:persimmon leaf, anti-atopic dermatitis, anti-oxidative, 2.4-Dinitrochlorobenzen(DNCB), leukotriene B 4 서 론 감나무(柿樹, Diospyros kaki Thunb)는 우리나라 중부 이남 지방에 주로 분포되어 있으며 생식하는 柿子 이외에도 柿葉, 柿花, 柿蔕 등은 예로부터 민간요법으로 사용되어 왔다. 그 중에서도 특히 柿葉은 주로 차로 이용되어 동의보감에 혈관 질환의 예방, 치료와 함께 자반증(혈관염증)에도 효과가 있다고 기록되어있다 1) . 柿葉에는 비타민 C, 아미노산, 핵산, 페놀성 화합물, 유리당 등 여러 물질들이 다양하게 함유되어 있다 2) . 柿葉은 총 페놀성 화합물 함량이 0.1~5.8%으로 다른 식물에 비하여 높으며 3) , 우수한 항산화능과 항균성이 확인되었고, superoxide dismutase(SOD) 활성에도 관여하는 것으로 알려
doi:10.6116/kjh.2012.27.1.41 fatcat:7psojqcxi5atlcounumsqptoji