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Inactivation of putative PKS genes can double geldanamycin yield in Streptomyces hygroscopicus 17997

Y.H. Li, M. Li, W.Q. He, Y.G. Wang, R.G. Shao
2013 Genetics and Molecular Research  
The putative polyketide biosynthesis (PKS) genes cos10 and pg10 were inactivated by insertion of a kanamycin-resistance gene into the genome of the geldanamycin-producing strain, Streptomyces hygroscopicus 17997. The resultant inactivation were confirmed by PCR analysis. The abilities of the PKS gene inactivation strains to produce geldanamycin were compared with the natural geldanamycinproducing strain, S. hygroscopicus 17997. The cos10-inactivated strain exhibited an unchanged ability to
more » ... ged ability to produce geldanamycin, but the pg10inactivated strain can produce twice the yield of the natural strain when grown under the same conditions. We propose that there is a sub-PKS pathway in the geldanamycin-producing strain, S. hygroscopicus 17997.
doi:10.4238/2013.june.21.3 pmid:23913387 fatcat:gbt7ncgstjed3e5qleg4bfyj44