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ANTIBACTERIAL EFFECTS OF SARANG SEMUT (MYRMECODIA PENDANS) FRACTIONS USING THREE DIFFERENT SOLVENTS TOWARD ENTEROCOCCUS FAECALIS CPS2

CIPTADHI TRI OKA BINARTHA, ENDANG SUPRASTIWI, DIDIK KURNIA, ANGGRAINI MARGONO, DEWA AYU NYOMAN PUTRI ARTININGSIH
2020 International Journal of Applied Pharmaceutics  
Objective: This study investigated the effect of antibacterial activity from sarang semut fractions with three different solvents, i.e. nonpolar (n-hexane),semipolar (ethyl acetate), and polar (water), to determine the minimum inhibitory concentration (MIC) on Enterococcus faecalis cps2.Methods: The fractions were extracted with a maceration method and a methanol solvent. The fractionation was performed with three groupsof solvent to obtain the n-hexane, ethyl acetate, and water fractions. The
more » ... ter fractions. The active compound from the best fraction group was identified using aphytochemical test, gas chromatography-mass spectrophotometry, and thin-layer chromatography. Each fraction group was divided into five differentconcentrations, i.e. 20%, 40%, 60%, 80%, and 100% and was assessed against E. faecalis cps2 with an agar diffusion method. Chlorhexidine 2% wasused as a positive control. The width of the inhibition zone was calculated.Results: The ethyl acetate group had the biggest inhibition zone of 21 mm in diameter compared to n-hexane and water, which was 15 mm and19 mm in diameter, respectively. The MIC value of the fraction with a 20% concentration of ethyl acetate was significantly different (P < 0.05) from then-hexane and water solvents in inhibiting the growth of E. faecalis cps2.Conclusion: The ethyl acetate fraction of sarang semut had a greater inhibitory effect on E. faecalis cps2. In addition, the antibacterial activity of thefraction increased with an increase in concentration.
doi:10.22159/ijap.2020.v12s1.iwdr01 fatcat:jbj5qezpj5g5jhw3z4w6zbizne