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Aim: Local prevalence estimates of problem drug use (PDU) are crucial in the process of assessment of drug situation and trends and for the planning of evidence-based policy responses. The aims of our study are twofold: to estimate the number of problem drug users (PDUs) in the Czech capital city in 2011, and to examine the usability of the capture-recapture method (CRM) modified for data from low-threshold programmes (LTPs) for drug users. Method: Six independent LTPs provided data fordoi:10.21101/cejph.a4096 pmid:27070968 fatcat:uvggxl23ifchxaszqi7xh37kpq