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Biological reactivity of nanoparticles: mosaics from optical microscopy videos of giant lipid vesicles

Jernej Zupanc, Andrej Dobnikar, Damjana Drobne, Janez Valant, Deniz Erdogmus, Erhan Bas
2011 Journal of Biomedical Optics  
Emerging fields such as nanomedicine and nanotoxicology, demand new information on the effects of nanoparticles on biological membranes and lipid vesicles are suitable as an experimental model for bio-nano interaction studies. This paper describes image processing algorithms which stitch video sequences into mosaics and recording the shapes of thousands of lipid vesicles, which were used to assess the effect of CoFe 2 O 4 nanoparticles on the population of
more » ... l-sn-glycero-3-phosphatidylcholine lipid vesicles. The applicability of this methodology for assessing the potential of engineered nanoparticles to affect morphological properties of lipid membranes is discussed. C 2011 Society of Photo-Optical Instrumentation Engineers (SPIE).
doi:10.1117/1.3533319 pmid:21361687 fatcat:2vuznk4anzd4jposga5q26nzqy