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A Homogeneous Split-Luciferase Assay for Rapid and Sensitive Detection of Anti-SARS CoV-2 Antibodies [post]

Igor Stagljar, Shawn Owen, Zhong Yao, Luka Drecun, Farzaneh Aboualizadeh, Sun Jin Kim, Zhijie Li, Emelissa J Valcourt, Kathy Manguiat, Heidi Wood, Simon Plenderleith, Lily Yip (+10 others)
2020 unpublished
To meet the urgent demand for better diagnostic tools to combat the ongoing COVID-19 pandemic, we developed a homogeneous immunoassay to detect IgG antibodies against SARS-CoV-2. This assay is based on a tri-part Nanoluciferase (tNLuc) approach, in which the spike protein of SARS-CoV-2 and protein G, fused respectively to two different tNLuc tags, are used as antibody probes. Target engagement of the probes allows reconstitution of a functional luciferase in the presence of the third tNLuc
more » ... he third tNLuc component. The assay is performed directly in liquid phase of patient sera and enables rapid, quantitative and low-cost detection. We show that tNLuc maintains a similar sensitivity to ELISA, while its readouts are highly consistent with various neutralizing antibody assays. This proof-of-principle study suggests potential applications in diagnostics and disease and vaccination management.
doi:10.21203/ fatcat:bhkv6fykejbzlcxl44ncklfsdq