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The chemical cleavage of apomyoglobins by the cyanogen bromide procedure1 has provided frag ments suitable for sequence analysis2, 3 and for studies of physical 4-6 and immunochemical pro perties 7. Offord 8 has used a peptide obtained from the cyanogen bromide cleavage of ribonuclease in studies aimed at the semi-synthesis of a protein. The purpose of this report is to present conditions that reliably separate pure peptide fragments produced from the apomyoglobins of several species. Adoi:10.1515/znc-1974-1-223 fatcat:7qm5335bx5eqbmiegkvwx7hsmi