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genotools SNP MANAGER: A New Software for Automated High-Throughput MALDI-TOF Mass Spectrometry SNP Genotyping

W. Pusch, K.-O. Kraeuter, T. Froehlich, Y. Stalgies, M. Kostrzewa
2001 BioTechniques  
Analysis of single nucleotide polymorphisms (SNPs) is a rapidly growing field of research that provides insights into the most common type of differences between individual genomes. The resulting information has a strong impact in the fields of pharmacogenomics, drug development, forensic medicine, and diagnostics of specific disease markers. The technique of matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) has been shown to be a highly suitable tool
more » ... hly suitable tool for the analysis of DNA. It supplies a very versatile method for addressing a high-throughput SNP genotyping approach. Here, we present the Bruker genotools SNP MANAGER, a new software tool suitable for highly automated MAL -DI-TOF MS SNP genotyping. The genotools SNP MANAGER administers the sample preparation data, calculates masses of allelespecific primer extension products, performs genotyping analysis, and displays the results. In the current study, we have used the genotools SNP MANAGER to perform an automated duplex SNP analysis of two biallelic markers from the promoter of the gene encoding the inflammatory mediator interleukin-6.
doi:10.2144/01301dd05 pmid:11196313 fatcat:dzxwrk5l5rggrgjaeg7fxajaay