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Transfer of Plasmid pAMβ1 Between Members of the Normal Microflora Inhabiting the Murine Digestive Tract and Modification of the Plasmid in aLactobacillus reuteriHost

M. A. McConnell, A. A. Mercer, G. W. Tannock
1991 Microbial Ecology in Health and Disease  
The fate of the broad-host-range, conjugative plasmid pAMPl in the digestive tract of mice was monitored by DNA-DNA hybridisations using a probe containing the ermAM determinant (macrolide-lincosamide-streptogramin type B resistance). Transfer of pAMPl from Lactobacillus reuteri to Enterococcus faecium occurred in the digestive tract of infant mice and from E. faecium to E.faecalis and to L. ferrnentum in the adult intestinal tract. Long-term administration of lincomycin to the animals resulted
more » ... he animals resulted in a modified form of pAMbl in the L. reuteri host. The modified plasmid was present in caecal isolates of the lactobacillus at higher concentration than in the stock culture used to inoculate the mice at the start of the experiment, and was maintained stably by the lactobacilli in vivo. In contrast, pAMP 1 was not stably maintained in the stock strain in the absence of antibiotic administration. The modified plasmid was no longer self-transmissible. Long-term administration of antibiotic to the animals resulted in erythromycin-resistant strains of Escherichia coli and eubacteria.
doi:10.3402/mehd.v4i6.7723 fatcat:hatnoafwvbfjrh2kxbgsziyori