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Development of real-time PCR tests for the detection of Tenebrio molitor in food and feed

Frédéric Debode, Aline Marien, Amaury Gerard, Frédéric Francis, Olivier Fumiere, Gilbert Berben
2017 Food Additives & Contaminants  
Insects are rich in proteins and could be an alternative source of proteins to feed animals and humans. Numerous companies have started the production of insects for feed purposes. In Europe, these processed animal proteins are not yet authorized by legislation, as many questions still need to be answered concerning this "novel food". Authorizations will be possible when methods of authentication of the products are available. In this study we propose real-time PCR methods for the specific
more » ... r the specific detection of the mealworm (Tenebrio molitor), one of the most widely used insects for food and feed production. Two PCR assays are proposed: a first one based on the wingless gene and a second one based on the cadherin gene. The PCR tests amplify fragments of 87 bp. These qualitative methods were tested according to several performance criteria. The specificity was tested on 34 insect species DNA but also on non-insect species including crustacean, mammals, birds and plants. The limit of detection was determined and was below 20 copies for the two PCR test. The applicability of the tests was demonstrated by the analysis of real-life processed samples containing Tenebrio molitor.
doi:10.1080/19440049.2017.1320811 pmid:28429650 fatcat:2o626n4vdfextjpt4rjbsz73ma