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The in vitro and in vivo development of mouse morulae after cryopreservation through different methods was examined. The slowfreezing involved an equilibration in 1.5M ethylene glycol (EG) and cooled at 0.5; 0.7; 1.0 or 1.2ºC/minute. The vitrification involved a 3 minutes equilibration in 20% EG and 60 seconds in solution containing 40% EG, 18% ficoll and 10.26% sucrose. The quick-freezing involved an equilibration in 3M EG + 0.3M sucrose for 5 minutes and 2 minutes in nitrogen vapor. In alldoi:10.1590/s1413-95962001000400003 fatcat:mh3sg2lrqbb4xb3goox6jz2ery