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In this study, quantitative and pattern recognition analysis for the quality evaluation of Magnoliae Flos using HPLC/ UV was developed. For quantitative analysis, eleven major bioactive lignan compounds were determined. The separation conditions employed for HPLC/UV were optimized using ODS C18 column (250 × 4.6 mm, 5 µm) with isocratic elution of acetonitrile and water with 1% acetic acid as the mobile phase at a flow rate of 1.0 mL/min and a detection wavelength of 278 nm. These methods weredoi:10.5012/bkcs.2010.31.11.3371 fatcat:5jx4nrh37za57latt2m2wonm54