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P4-S3.02 Subtyping of Treponema pallidum strains by sequence analysis of tp0279 and tp0548

A. Pillay, C. Y. Chen, M. G. Morshed, S. Philip, R. C. Ballard
2011 Sexually Transmitted Infections  
Subtyping of T pallidum, using a previously-published technique, was based on the molecular characterisation of two variable treponemal repeat genes, arp and tpr. Results Macrolide resistance profiles and the subtype distribution for all 60 DNA samples were obtained. The mean age of all participants was 27.4 years (range 18e43; SD 5.24) of which 55 (92%) were male and 5 (8%) were female. None of the samples analysed contained the 23S rRNA gene point mutation that confers macrolide resistance. A
more » ... olide resistance. A total of eight arp repeat sizes, 8 RFLP patterns and a combined total of 17 subtypes were identified in this study population. The most common subtypes were 14d (43%), followed by 17d (13%), 14b (7%), 22b (5%) and 23b (5%). Conclusions This is the first South African study to examine both macrolide-resistance profiles and the subtype distribution of syphilis strains using molecular techniques. Macrolide-resistant T pallidum strains appear to be uncommon in South Africa compared to more developed countries. Our results indicated subtype 14d to be the predominant circulating T pallidum strain in South Africa and there seem to be a high degree of genetic heterogeneity within this population.
doi:10.1136/sextrans-2011-050108.517 fatcat:4narayc425eeff74pztmh5xfy4