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Poor transduction efficiency of human hematopoietic progenitor cells by a high-titer amphotropic retrovirus producer cell clone

L C Xu, H A Young, M Blanco, S Kessler, A B Roberts, S Karlsson
1994 Journal of Virology  
The transduction efficiency of human bone marrow CD34+ cells with supernatants from the retrovirus producer cell clone PA317/LGSN 16 was only one-fifth of that with supernatants from GP+envAml2/LGSN 15, even though both producers had similar infection titers on 3T3 cells. PA317/LGSN 16-conditioned medium inhibited the proliferation of the bone marrow CD34+ cells, and this inhibitory effect was partially blocked by anti-transforming growth factor 13 antibodies. These studies suggest that
more » ... uggest that cytokine secretion plays a role in the 7634 Vol. 68, No. 11 on May 9, 2020 by guest
doi:10.1128/jvi.68.11.7634-7636.1994 fatcat:dxaiajauijdc5diq4yrpnpn43e