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Laboratory Diagnosis of CMV Infection: A Review

Munira Jahan
2012 Bangladesh Journal of Medical Microbiology  
infection in high-risk patients prior to the onset of clinical disease is preferred 1,2 . Since rapid and sensitive technique for diagnosis of CMV infection is of vital importance for the management of immunocompromised patients, a number of rapid and sensitive virus detection methods have been developed. These includes DNA probe techniques 3 , Polymerase chain reaction (PCR) 4,5 , CMV antigen detection in biopsies and bronchoalveolar lavage 6,7 , and immunofluorescence technique for detection
more » ... ique for detection of CMV early antigens in cell 7-,10 . Moreover, an assay has recently been developed for CMV antigenemia based on the detection of CMV immediate early antigen (pp 65 ) in circulating leucocytes 1,10-12 . Electron microscopy Electron microscopy has been used to identify CMV in urine and is 25 %-95% as sensitive as viral culture in congenitally infected infants 13,14 . Monplaisir 14 (1972) used ultracentrifugation, whereas Lee et al 13 (1978) used a simpler, pseudoreplica technique. This method was 95% sensitive when the viral infectivity titer was 10 4 /ml 13 . Below this titer the sensitivity of the technique was only 25%. Since many infants >6 months old and adults (especially those who are immunocompetent) have titer > 10 4 / ml, the use of electron microscopy is limited. Electron microscopy is now rarely employed to identify CMV in clinical virology laboratories. Lab Diagnosis a CMV Infection: A Review Jahan M 40 Bangladesh
doi:10.3329/bjmm.v4i2.10831 fatcat:ac7ui2kv4jdxvhqpeownxihplq