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The immunogenicity and protective efficacy of an attenuated isolate of Borrelia burgdorferi, the agent of Lyme disease, were evaluated. An isogenic pair of isolates of strain HB19 differed in the expression of flagella; neither produced systemic infection or persisted in the skin of mice. In a comparison of intradermally administered live flagella-bearing and flagella-less cells, the flagellaless isolate was equal to or superior to the flagella-bearing wild type in eliciting growth-inhibitingdoi:10.1093/infdis/173.5.1184 pmid:8627071 fatcat:durrpswnsbfjnbvlbiom23j3om