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Interaction of Leishmania gp63 with cellular receptors for fibronectin

A Brittingham, G Chen, B S McGwire, K P Chang, D M Mosser
1999 Infection and Immunity  
Because gp63 has been shown to possess fibronectin-like properties, we examined the interaction of gp63 with the cellular receptors for fibronectin.  ...  In both cases, this interaction depended on gp63 but occurred independently of the SRYD sequence of gp63, because parasites expressing gp63 with a mutated SRYD sequence bound to macrophages and alpha4/  ...  ACKNOWLEDGMENTS We thank Robert McMaster for the generous gift of gp63. This work was supported by Public Health Service grants AI24313 (to D.M.M.) and AI20486 (to K.  ... 
pmid:10456889 pmcid:PMC96767 fatcat:gcmeesej7ndptbhf3qfp7wlxvi

Participation of Trypanosoma cruzi gp63 molecules on the interaction with Rhodnius prolixus

Karina M. Rebello, Livia A. Uehara, Vítor Ennes-Vidal, Aline S. Garcia-Gomes, Constança Britto, Patrícia Azambuja, Rubem F. S. Menna-Barreto, André L. S. Santos, Marta H. Branquinha, Claudia M. d'Avila-Levy
2019 Parasitology  
Although gp63 homologues are highly expanded in the T. cruzi genome, and are implicated in parasite–mammalian host interaction, its role in the insect vector has never been explored.  ...  The parasite molecules involved in vector interaction have been little investigated.  ...  The use of divalent metal chelators as an indirect approach to access gp63 functions in the insect interaction has been previously applied by our research group, for instance while assessing the interaction  ... 
doi:10.1017/s0031182019000441 pmid:31057143 pmcid:PMC6604109 fatcat:isr72qtbvzh6zinnydogmbs26a

Study of Functionalized Gold Nanoparticles with Anti-gp63 IgG Antibody for the Detection of Glycoprotein gp63 in Membrane Surface of Leishmania Genus Parasites

Pedro Rodríguez, Humberto Rojas, Miguel Medina, Jazzmin Arrivillaga, Yosue Francisco, Fracehuli Dager, Vincent Piscitelli, Manuel Caetano, Alberto Fernández, Jimmy Castillo
2013 American Journal of Analytical Chemistry  
The nanoparticles were functionalized with anti-gp63 lgG antibody in order to study the interaction with the glycoprotein component gp63 (63 kDa) present on the membrane surface of Leishmania genus parasites  ...  The functionalized AuNPs showed potential as a spectrometric indicator of the parasite existence, both by the detection of the presence of the gp63 in solution and through the specific interaction with  ...  Nanoparticles Functionalization Interaction of Functionalized Nanoparticles with gp63 The interaction of the AuNPs-PEG-anti-gp63 array (FAuNPs) was studied by putting in contact this array and the gp63  ... 
doi:10.4236/ajac.2013.47a014 fatcat:kiuceyin7rbw7p4bmm66p6jaxa

Identification of potent natural compounds in targeting Leishmania major CYP51 and GP63 proteins using a high-throughput computationally enhanced screening

Deepak A. Yaraguppi, Sanjay H. Deshpande, Zabin K. Bagewadi, Uday Muddapur, Sushil Anand, Santosh B. Patil
2020 Future Journal of Pharmaceutical Sciences  
The molecular docking of the resulted compounds was carried out with therapeutic targets CYP51 and GP63 proteins.  ...  The interaction of compounds with the target GP63 can be seen in Fig. 5 .  ...  The binding energy of interaction for GP63 with 46173719 showed − 8.5 and docking score of GP63 with UNPD86509 showed − 7.8.  ... 
doi:10.1186/s43094-020-00038-w fatcat:6gt7qgke3rhjxgm6nauoxzge6q

TheLeishmaniaSurface Protease GP63 Cleaves Multiple Intracellular Proteins and Actively Participates in p38 Mitogen-activated Protein Kinase Inactivation

Maxime Hallé, Maria Adelaida Gomez, Matthew Stuible, Hidehisa Shimizu, W. Robert McMaster, Martin Olivier, Michel L. Tremblay
2008 Journal of Biological Chemistry  
Cellular and in vitro assays using pharmacological protease inhibitors, recombinant enzyme, and genetically modified strains of L. major identified the parasite protease GP63 as the principal catalyst  ...  Protein degradation occurred in cells incubated with Leishmania extracts in the absence of intact parasites, suggesting a mechanism permitting transfer of functional GP63 into the intracellular space.  ...  We then showed that TAB1 is cleaved in a GP63-dependent manner, generating fragments unable to interact with p38.  ... 
doi:10.1074/jbc.m805861200 pmid:19064994 pmcid:PMC2652307 fatcat:w2e2bmqmjvhcvpcyfnrvgszb7y

The ubiquitous gp63-like metalloprotease from lower trypanosomatids: in the search for a function

André L.S. Santos, Marta H. Branquinha, Claudia M. D'Avila-Levy
2006 Anais da Academia Brasileira de Ciências  
The gp63 homologues found in lower trypanosomatids seem to play essential roles in the nutrition as well as in the interaction with the insect epithelial cells.  ...  A myriad of functions have been described for the gp63 from Leishmania spp. when the metacyclic promastigote is inside the mammalian host.  ...  The possibility exists that gp63 may function differently for these two distinct Leishmania species in their interactions with different invertebrate vector species. gp63 homologues have been initially  ... 
doi:10.1590/s0001-37652006000400006 pmid:17143406 fatcat:jftqa2oimjffrp6o4mxd4yesbe

The gp63 Gene Cluster Is Highly Polymorphic in Natural Leishmania (Viannia) braziliensis Populations, but Functional Sites Are Conserved

Lilian S. Medina, Bruno Araújo Souza, Adriano Queiroz, Luiz Henrique Guimarães, Paulo Roberto Lima Machado, Edgar M Carvalho, Mary Edythe Wilson, Albert Schriefer, Jude Marek Przyborski
2016 PLoS ONE  
GP63 or leishmanolysin is the major surface protease of Leishmania spp. involved in parasite virulence and host cell interaction.  ...  Genomic DNA was extracted, and 405 bp fragments, including sequences encoding the putative macrophage interacting sites, were amplified from gp63 genes of all isolates.  ...  (V.) braziliensis host cell interaction.  ... 
doi:10.1371/journal.pone.0163284 pmid:27648939 pmcid:PMC5029894 fatcat:mctsdj7n25hnvabjfaskgk2hsu

Migration through the Extracellular Matrix by the Parasitic Protozoan Leishmania Is Enhanced by Surface Metalloprotease gp63

B. S. McGwire, K.-P. Chang, D. M. Engman
2003 Infection and Immunity  
This correlates with gp63 degradation of extracellular matrix components, such as collagen type IV and fibronectin, and suggests an important role for gp63 in the pathogenesis of leishmaniasis.  ...  Leishmania species engineered to express high levels of the surface metalloprotease gp63 have enhanced capacity of migration through extracellular matrix in vitro.  ...  The 63-kDa glycoprotein gp63 is a zinc-dependent metalloprotease found on the surface of the parasite that facilitates complement inactivation in serum (3) , interaction with the host macrophage (2,  ... 
doi:10.1128/iai.71.2.1008-1010.2003 pmid:12540585 pmcid:PMC145380 fatcat:fd4x5gc2ovbjdjkstcpkg5ozvu

African Trypanosomes Have Differentially Expressed Genes Encoding Homologues of theLeishmaniaGP63 Surface Protease

Najib M. A. El-Sayed, John E. Donelson
1997 Journal of Biological Chemistry  
These results lead to a reconsideration of the potential functions of GP63, inasmuch as African trypanosomes are not known to interact with macrophages and do not have an intracellular stage during their  ...  of mRNAs for GP63 and variant surface glycoprotein is about 1:150.  ...  A number of different functions have been ascribed to Leishmania GP63s, several of which implicate GP63 in the interaction between the infective promastigote and the macrophage.  ... 
doi:10.1074/jbc.272.42.26742 pmid:9334260 fatcat:wvpwydavazey3obocry64pcgpa

Involvement ofLeishmania donovani major surface glycoprotein gp63 in promastigote multiplication

Sanjeev Pandey, Phuljhuri Chakraborti, Rakhi Sharma, Santu Bandyopadhyay, Dwijen Sarkar, Samit Adhya
2004 Journal of Biosciences  
By expressing gp63 antisense RNA from an episomal vector in L. donovani promastigotes, gp63-deficient transfectants were obtained.  ...  These observations support an additional role of gp63 in promastigote multiplication; the fibronectin-like properties of gp63 may be important in this process.  ...  interaction.  ... 
doi:10.1007/bf02702557 pmid:15286399 fatcat:s4qvsyl2p5dwhd7htdibdizumu

Specific pseudorabies virus infection of the rat visual system requires both gI and gp63 glycoproteins

M E Whealy, J P Card, A K Robbins, J R Dubin, H J Rziha, L W Enquist
1993 Journal of Virology  
We also show that gI and gp63 physically interact, as demonstrated by pulse-chase and sucrose gradient sedimentation experiments.  ...  Efficient export of either gI or gp63 from the endoplasmic reticulum to the Golgi apparatus in a fibroblast cell line requires the presence of both proteins.  ...  The glycoprotein complex was stabilized by noncovalent interactions, most probably hydrophobic interactions.  ... 
pmid:8389905 pmcid:PMC237743 fatcat:6fguyddkvrb6vodz2hzztoixh4

Variation in Leishmania chemokine suppression driven by diversification of the GP63 virulence factor [article]

Alejandro L. Antonia, Amelia T. Martin, Liuyang Wang, Dennis C. Ko
2021 bioRxiv   pre-print
(V.) panemensis primarily encodes non-CXCL10-cleaving gp63 alleles. In contrast, L.  ...  We found the putative CXCL10 binding site to be located in a region of gp63 under significant positive selection and that it varies from the L. major wild-type sequence in all gp63 alleles identified in  ...  the protein-protein docking interaction.  ... 
doi:10.1101/2021.02.01.429140 fatcat:5e44c462jfd27ha42s3b3kav44

Leishmania-Induced Inactivation of the Macrophage Transcription Factor AP-1 Is Mediated by the Parasite Metalloprotease GP63

Irazú Contreras, María Adelaida Gómez, Oliver Nguyen, Marina T. Shio, Robert W. McMaster, Martin Olivier, David L. Sacks
2010 PLoS Pathogens  
Immunoprecipitation of c-Jun from nuclear extracts showed that GP63 interacts, and cleaves c-Jun at the perinuclear area shortly after infection.  ...  Furthermore, the cleavage of c-Jun is dependent on the expression and activity of the major Leishmania surface protease GP63.  ...  One possible explanation is that the structural conformation of this protein renders these sites unavailable for GP63-Jun D interaction. GP63 is known to interact with various substrates.  ... 
doi:10.1371/journal.ppat.1001148 pmid:20976196 pmcid:PMC2954837 fatcat:ynjjsraaz5fg3lihc74szhhwkq

Complement receptor type 3 (CR3) binds to an Arg-Gly-Asp-containing region of the major surface glycoprotein, gp63, of Leishmania promastigotes

D. G. Russell
1988 Journal of Experimental Medicine  
Isolation of gp63 and LPG. The promastigote glycoprotein gp63 was isolated by Con A chromatography ofa detergent extract from a crude membrane preparation as described previously (10, 24).  ...  Soluble gp63, lacking the phospholipid anchor, was produced by incubating gp63 bound to Con A-Sepharose, with 5 U/ml phospholipase C from Bacillus ceres (type III ; Sigma Chemical Co., St.  ...  The above data indicate that gp63 contains a sequence that can interact directly with CR3 .  ... 
doi:10.1084/jem.168.1.279 pmid:3294332 pmcid:PMC2188978 fatcat:k7pqamkeojduvlft7mmoxjphua

An evolutionary analysis of trypanosomatid GP63 proteases

Lina Ma, Kaifu Chen, Qingshu Meng, Qingyou Liu, Petrus Tang, Songnian Hu, Jun Yu
2011 Parasitology Research  
The trypanosomatid GP63 proteases are known to be involved in parasite-host interaction and exhibit strong sequence and structural similarities to those of their hosts and insect vectors.  ...  ., we annotated all their GP63 proteases and divided highly duplicated T. cruzi GP63 proteases into four novel groups according to sequence features.  ...  These results suggest that GP63 proteases may indeed play significant roles in host-parasite interactions.  ... 
doi:10.1007/s00436-011-2348-x pmid:21503641 fatcat:3jiphalhhndxtc4oyn2zmqybeq
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