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A large number of interesting mutations affecting development and organogenesis have been identified through genetic screens in zebrafish. Mapping of these mutations to a chromosomal region can be rapidly accomplished using half-tetrad analysis. However, knowledge of centromere-linked markers on every chromosome is essential to this mapping method. Centromeres on all 25 linkage groups have been mapped on the RAPD zebrafish genetic map. However, species specificity and the lack of codominancedoi:10.1006/geno.2000.6233 pmid:10945477 fatcat:5ibjcysvrnbftckafch2d7uxoy
more »... k of codominance make RAPD markers less practical for mapping than microsatellite-based markers. On the microsatellite-based genetic map, centromere-linked markers have been identified for 19 linkage groups. No direct evidence has been published linking microsatellite markers to the centromeres of linkage groups 3, 4, 6, 7, 13, and 20. Therefore, we compared the microsatellite-based genetic map with the RAPD map to identify markers most likely linked to the centromeres of these 6 linkage groups. These candidate markers were tested for potential centromere linkage using four panels of halftetrad embryos derived by early-pressure treatment of eggs from four different female zebrafish. We have identified microsatellite markers for linkage groups 3, 4, 6, 7, 13, and 20 to within 1.7 cM of their centromeres. These markers will greatly facilitate the rapid mapping of mutations in zebrafish by half-tetrad analysis.
include Vaccinium species (Red Huckleberry [Vaccinium parvifolium Smith], Ovalleaved Blueberry [Vaccinium ovalifolium Smith]), Salal (Gaultheria shallon Pursh), and Red El der berry (Sambucus racemosa L. ... 4 -2 8 J u n 2 9 -J u l 3 J u l 4 -8 J u l 9 -1 3 M a y 2 0 -2 4 M a y 2 5 -2 9 M a y 3 0 -J u n Appendix 1. ... M a y 3 0 -J u n 3 J u n 4 -8 J u n 9 -1 3 J u n 1 4 -1 8 J u n 1 9 -2 3 J u n 2 4 -2 8 J u n 2 9 -J u l 3 J u l 4 -8 J u l 9 -1 3 M a y 2 0 -2 4 M a y 2 5 -2 9 M a y 3 0 -J u n 3 J u n 4 -8 J u n 9 -1 ...doi:10.22621/cfn.v133i4.2277 fatcat:lba2qrjwl5bgxpvsmtoadwl6yu
b1 K b2 ͓L ͔ 2 ͒ (Eq. 7) and the fraction of protein bound with two ligands, F 2 , is, F 2 ϭ K b1 K b2 ͓L ͔ 2 /͑1 ϩ K b1 ͓L ͔ ϩ K b1 K b2 ͓L ͔ 2 ͒ (Eq. 8) so that Equation 5 becomes Q t ϭ ͓CRP͔ t V ͓F ... For ligand binding to two sites (Yang, 1990) , the fraction of protein bound with one ligand, F 1 , where [L] is the free ligand concentration is from Equations 1-3, F 1 ϭ K b1 ͓L ͔/͑1 ϩ K b1 ͓L ͔ ϩ K ...doi:10.1074/jbc.270.37.21679 pmid:7665583 fatcat:5xjaqdjkwzhidlu3y4g77kgddy
The Journal of the Kyushu Dental Society
v 2) 3 1. 4 1/ 5 ', 6 1i 7} 8 9 10 11 12 13 )] ア マ ル ガ ム 圧 縮 強 さ の 腐 食 に よ る 変 化 九 州 歯 科 大 学 歯 科 理 工 学 講 座 ( 主 任 ：林 一 郎 教 授 ) B ．Keith Moore ， Marjorie L ． ... -ttt Code M M* v v* s s* Ss* D D* E I s, tt ' -tttt Manufacturer L. D. Caulk L. D. Caulk s. s. white S. S. ...doi:10.2504/kds.35.481_10 fatcat:lhooqtvh5ndnpd6mtrxat5klhq
G, gill; M, muscle; B, brain; L, liver; K, kidney; R, retina; and T, testes. Samples were scraped from deparaffinized sections from two fish (a and b) from each experimental condition as indicated. ... PCR was performed using 5 µ L DNA in a 20 µ L reaction containing 0.2 µ M each primer and amplified for 35-40 cycles using TaqDNA polymerase (Fisher Scientific) as previously described (2) . ... A 25-gauge needle was used to microdissect 1-2 mm 2 regions of tissue into 100 µ L DNA extraction buffer (7) . DNA from zebrafish tail fin clips was also prepared using a standard protocol (2) . ...doi:10.2144/02322st03 pmid:11848405 fatcat:khaoibb5gbfjlaizrbjl2esn7u
Briefly, 0.5 l of the lysate was incubated with end-labeled probe for 30 min at room temperature, and the reaction mixture was analyzed by 5% nondenaturing gel electrophoresis. ...doi:10.1074/jbc.274.30.20869 pmid:10409629 fatcat:d7k3qats35bkxihltmuoobeona
This paper reports the applicability of a dimethyl sulfoxide (DMSO) solution of stannous acetate (Sn(OAc) 2 /DMSO) for sensitization in electroless deposition (ELD). Silver ELD was examined on nanostructured surfaces derived from polystyrene-blockpoly(methylmethacrylate) via sensitization with an aqueous or DMSO solution of Sn(OAc) 2 or SnCl 2 , activation with aqueous Ag[(NH 3 ) 2 ] + , followed by immersion in an aqueous solution containing [Ag(NH 3 ) 2 ] + and tartrate. Sensitization withdoi:10.1149/2.1391810jes fatcat:izhex7eltfhmll2aht3n4qbmiq
PURPOSE. To determine the relationship between the severity of distance and near-vision impairment on vision-specific quality of life (QoL) and depression in residential care residents. METHODS. Residents from three low-level residential care facilities in Victoria (Australia) were recruited. All participants were assessed for cognitive impairment, distance and near-vision impairment (VI), and depression. Sociodemographic and other clinical data were also collected. The subscales of the Nursingdoi:10.1167/iovs.08-3294 pmid:19357359 fatcat:pmh5xbc2l5dc7ok665pkoqkxka
more »... ales of the Nursing Home Vision-Targeted Health-Related Quality-of-Life questionnaire (NHVQoL) were the main outcome measures and were validated by Rasch Analysis. RESULTS. Seventy-six residents were enrolled. The mean Ϯ SD of the participants' age was 83.9 Ϯ 9.9 years, and most were women (n ϭ 44; 60%); 46.4% (n ϭ 35) had binocular presenting VI (Ͻ6/12), and 59% (n ϭ 44) had at least mild near VI (worse than N8); 16% (n ϭ 14) recorded depression symptoms, although depression was not associated with VI (P Ͼ 0.05). In linear regression models, distance and near VI was independently associated with poorer QoL on seven of the eight subscales of the NHVQoL scale (P Ͻ 0.05). The ␤ coefficients ranged from Ϫ12.3 to Ϫ80.2, which suggests that, on average, people with vision loss had poorer QoL, ranging between 12 and 80 points (scale range: 0 -100) than did those with no VI. The QoL aspects most affected by vision loss were related to general vision, reading, hobbies, emotional wellbeing, and social interaction. CONCLUSIONS. VI remains a major form of disability in individuals living in residential care facilities and affects vision-specific functioning and socioemotional aspects of daily living. A larger study is needed to confirm these findings. (Invest Ophthalmol Vis Sci. 2009;50:4103-4109)
The Journal of the Kyushu Dental Society
Caulk L. D, Caulk S, S, White S. S, White Kerr Mfg., Kerr Mfg., Shofu Dentai Johnson & Johnson & L, D. Caulk Shelf:y"Dental tt tt Manufacturer .r tt- t , Milford, DE, U.S.A. ... KyushuDental Society -490 - Table l l Amalgamalloy.s. tested VWH*ftn.i,i ng35#li'i4-}TJ-l981 Alloy Code Lathe cut Ag-Sn Spherical Ag-Sn Lathe cut Ag-Sn admixed with spherical <Ag-Cu ...doi:10.2504/kds.35.489 fatcat:bmqfc7v3ybd4tdidxrb6bfaww4
L. J. Pluim et al, in 1984  measured the disintegration rate of glass ionomer cement and zinc phosphate cement. ...doi:10.14219/jada.archive.1987.0093 pmid:3470375 fatcat:omit4g2xlrexbbrabto2jn3qnu
The purpose of this study was to compare the contents of root canals obturated with gutta percha and AH-26 sealer (Dentsply, Tulsa, OK) to canals obturated with the Resilon and Epiphany (Pentron, Wallingford, CT) system. Canal contents were assessed by determining the percentage of canal space occupied by core material, sealer, voids, and debris. Forty extracted human maxillary anterior teeth were instrumented, and the teeth were randomly assigned to either the gutta percha/AH 26 group or thedoi:10.1016/j.joen.2007.07.021 pmid:17963964 fatcat:i4gmmp3y4vav3ffo3nz2nwmivu
more »... H 26 group or the Epiphany/ Resilon group. Canals were obturated, and the teeth were subsequently embedded in resin and sectioned horizontally at 2, 4, and 6 mm from the anatomic apex. Sections were photographed by using a low vacuum scanning electron microscope. Image-J (Wayne Rasband; National Institute of Health, Bethesda, MD) software was used to quantify the proportion of core material, sealer, voids, and debris in each canal. Percentages and statistical comparisons for each method were compared.
Using corn (Zea mays L.) as a companion weed control was acceptable for establishing switchgrass crop could provide potential for high biomass production during and big bluestem. ... Martin et al. (1982) found that switchgrass and big bluestem were tolerant to preemergence applications of up Switchgrass (Panicum virgatum L.) and big bluestem (Andropogon to 3.4 kg active ingredient ... Pers.] and wild proso millet (Panicum miliaceum Harvest L.). ...doi:10.2134/agronj1998.00021962009000050004x fatcat:yy3effqprjdajkioy2l5bdd2jq
Q t ϭ n ͓CRP͔ t ⌬H b V ͕1 ϩ ͓L͔ t /n ͓CRP͔ t ϩ 1/nK b ͓CRP͔ t Ϫ ͓͑1 ϩ ͓L͔ t /n ͓CRP͔ t ϩ 1/nK b ͓CRP͔ t ͒ 2 Ϫ 4͓L͔ t /n ͓CRP͔ t ͔ 1/2 ͒/2 (Eq. 2) where Q t is the total heat, [L] t is the total ligand ... The volume of the protein solution cell was 1.385 ml, and the volume of the injectant ranged from 4 to 10 l. ...doi:10.1074/jbc.271.35.21273 pmid:8702903 fatcat:bqxg4ezjjvdqtgypmp4um7hmxa
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