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Development and Validation of Nutrition Literacy Questionnaire for the Chinese Elderly

Sumiya Aihemaitijiang, Chen Ye, Mairepaiti Halimulati, Xiaojie Huang, Ruoyu Wang, Zhaofeng Zhang
2022 Nutrients  
(1) Background: Improving nutrition literacy is crucial for maintaining a healthier state of the elderly to achieve healthy ageing. Therefore, it is necessary to develop a Nutrition Literacy Questionnaire for the Chinese Elderly (NLQ-E). (2) Methods: an NLQ-E was developed according to the core components of nutrition literacy for the elderly. Internal consistency, exploratory factor analysis (EFA) and confirmatory factor analysis (CFA) were used to validate the reliability and validity of the
more » ... LQ-E. A cross-sectional study of 1490 elderly people was used to analyze the application of the NLQ-E. (3) Results: The NLQ-E was constructed with 3 domains (knowledge and understanding, healthy lifestyle and dietary behavior and skill), with a total of 25 questions. The overall NLQ-E had acceptable reliability and validity (Cronbach's α = 0.678, χ2/DF = 4.750, RMSEA = 0.045, PCFI = 0.776 and PNFI = 0.759). The average nutrition literacy score of the subjects in this cross-sectional study was 65.95 (65.95 ± 10.93). The OR between the nutrition literacy score and multimorbidity was 0.965 (95% CI: 0.954, 0.976); (4) Conclusions: We developed and validated the NLQ-E and found that the nutrition literacy level of the Chinese elderly was generally low. This study is of great value to improve the nutrition literacy of the elderly and effectively prevent nutrition-related chronic diseases and multimorbidity.
doi:10.3390/nu14051005 pmid:35267979 pmcid:PMC8912634 fatcat:da5czp4l5vfbxcmp3sjj5agyle

Coupling between the DEAD-box RNA helicases Ded1p and eIF4A

Zhaofeng Gao, Andrea A Putnam, Heath A Bowers, Ulf-Peter Guenther, Xuan Ye, Audrey Kindsfather, Angela K Hilliker, Eckhard Jankowsky
2016 eLife  
Eukaryotic translation initiation involves two conserved DEAD-box RNA helicases, eIF4A and Ded1p. Here we show that S. cerevisiae eIF4A and Ded1p directly interact with each other and simultaneously with the scaffolding protein eIF4G. We delineate a comprehensive thermodynamic framework for the interactions between Ded1p, eIF4A, eIF4G, RNA and ATP, which indicates that eIF4A, with and without eIF4G, acts as a modulator for activity and substrate preferences of Ded1p, which is the RNA remodeling
more » ... unit in all complexes. Our results reveal and characterize an unexpected interdependence between the two RNA helicases and eIF4G, and suggest that Ded1p is an integral part of eIF4F, the complex comprising eIF4G, eIF4A, and eIF4E.
doi:10.7554/elife.16408 pmid:27494274 pmcid:PMC4990422 fatcat:7wnowqoxjfcnjflg5su5cpmemu

Development and Validation of a Food and Nutrition Literacy Questionnaire for Chinese Adults

Yaqin Zhang, Zhaofeng Zhang, Meihong Xu, Sumiya Aihemaitijiang, Chen Ye, Wenli Zhu, Guansheng Ma
2022 Nutrients  
The participants who were female, with higher education levels, stable marital status (never married or married), healthcare-related education or work experience (YES), or suffering from chronic disease  ...  Variable values: Sex (Male = 0, Female = 1); Healthcare related education or work experience (No = 0, Yes = 1). -: no data; *: Exchange rate of CNY to USD is about RMB 640 equal to USD 100. & : Suffered  ... 
doi:10.3390/nu14091933 pmid:35565900 pmcid:PMC9104569 fatcat:xperoxia6vghtm6ybqf74o5nky

MarkovHC: Markov hierarchical clustering for the topological structure of high-dimensional single-cell omics data [article]

Zhenyi Wang, Yanjie Zhong, Zhaofeng Ye, Lang Zeng, Yang Chen, Minglei Shi, MinPing Qian, Michael Q Zhang
2020 bioRxiv   pre-print
Then we define attractors and attractive basins of higher levels as 1 1 2 3 Zhenyi Wang, #,1 Yanjie Zhong, 3,5 Zhaofeng Ye, 2 Lang Zeng, 6 Yang Chen, 1 Minglei 4 Shi, 2 Minping Qian* ,3 and Michael  ... 
doi:10.1101/2020.11.04.368043 fatcat:kxyvbpjy2vbm3gx35jw2hvgnru

Oat β Glucan Ameliorates Renal Function and Gut Microbiota in Diabetic Rats

Ruoyu Wang, Zhaofeng Zhang, Sumiya Aihemaitijiang, Chen Ye, Mairepaiti Halimulati, Xiaojie Huang, Haoyuan Qin
2022 Frontiers in Nutrition  
Diabetic nephropathy is a severe complication of diabetes and the leading cause of end-stage renal disease and death. Therefore, we must find effective prevention and treatment approaches to the problem. Oat has a long history of use for its nutritional and medicinal properties, such as maintaining physiological blood glucose levels. Oat β glucan is one of the major bioactive substances produced by oat. However, the protective effects of oat β glucan on diabetic nephropathy are still unclear.
more » ... is study generated a rat model of diabetic nephropathy to explore the potent therapeutic ability and mechanism of oat β glucan in renal function by 16S rRNA genes sequencing. Diabetic nephropathy model was established in forty rats by left nephrectomy and single intraperitoneal injection of streptozotocin. These rats were randomly divided into the model group and three oat β glucan intervention groups. Twenty rats underwent sham operation and were randomly divided into normal control group and oat β glucan control group. Animals were treated by oral gavage for 8 consecutive weeks. The results showed that oat β glucan reduced blood glucose level and improved renal function (P < 0.05). Oat β glucan significantly improved serum inflammatory levels (P < 0.05). The diversity of intestinal microflora in diabetic nephropathy rats decreased with time prolongation, while oat β-glucan reversed the result. Compared with the model group at week 8, the abundances of Eubacterium, Butyricicoccus, and Ruminococcus were elevated significantly after oat β glucan intervention (P < 0.05). Correlation analysis indicated that abundances of Eubacterium, Butyricicoccus, and Ruminococcus were significantly negatively correlated with the levels of renal impairment markers. In summary, the findings of this study showed that oat β glucan can increase the diversity of intestinal flora, regulate the composition of intestinal flora, modulate intestinal flora metabolism, alleviate the inflammatory response, and further delay the development of diabetic nephropathy. Therefore, oat β glucan has the potential to be developed into the novel and safe drug for diabetic nephropathy.
doi:10.3389/fnut.2022.875060 pmid:35614982 pmcid:PMC9125244 fatcat:z6cf4ca7jza3fepfva2wo4iiu4

Analysis of Load Transfer Law of Short Pile-Net Composite Foundation for High-Speed Railway

Shiyou Li, Liming Ye, Hong Jiang, Zhaofeng Chen, Biao Li
2022 Shock and Vibration  
Although pile-net composite foundation has been widely used in the construction of high-speed railway, load transfer characteristics of its structural system during the filling of roadbed are seldom studied, so field studies are conducted in the Gan-Long (Ganzhou-Longyan) high-speed railway DK86 + 998.0–DK87 + 191.2 test section, aiming to explore the bearing characteristics of a short pile-net composite foundation over low to medium compressible soil. The study encompasses a field measurement
more » ... f Earth pressure and pore water pressure which lasted almost two years, and a systematic analysis of variation law of soil pressure between piles and their tops and that of pore pressure in reinforcement zone and retaining layer in medium and low compressibility soil area. The results suggest that the soil in the test section bears the characteristic of a low water content, low porosity ratio, and high liquid limit, with compression factor being approximately 0.25, and test values of the natural soil pressure and the lateral distribution are close to the theoretical values. Soil pressure at the top of the piles is higher than that between the piles by approximately 2.4 times because of the differences in pile-net rigidity. Besides, the soil pressure of the pile-net composite foundation follows the pattern of "jagged" in the transverse direction of the roadbed. The filling load of the composite foundation soil arch is 80 kPa and the composite foundation reaction coefficient is 6.4 kN/m3 when the filling is stable. The pile-soil stress ratio at the shoulder and the center of the line is 3.2 and 2.8, respectively. The change of the hole pressure at the pile end point is larger than that at the reinforcement area, and the side verification short pile can effectively transmit the additional stress of the upper part to the pile end soil layer.
doi:10.1155/2022/6120609 fatcat:axofay7tvbcppmu2dligv4bhse

Symmetry breaking during homodimeric assembly activates an E3 ubiquitin ligase

Zhaofeng Ye, Patrick G. Needham, Samuel K. Estabrooks, Susan K. Whitaker, Brandon L. Garcia, Saurav Misra, Jeffrey L. Brodsky, Carlos J. Camacho
2017 Scientific Reports  
C-terminus of Hsc/p70-Interacting Protein (CHIP) is a homodimeric E3 ubiquitin ligase. Each CHIP monomer consists of a tetratricopeptide-repeat (TPR), helix-turn-helix (HH), and U-box domain. In contrast to nearly all homodimeric proteins, CHIP is asymmetric. To uncover the origins of asymmetry, we performed molecular dynamics simulations of dimer assembly. We determined that a CHIP monomer is most stable when the HH domain has an extended helix that supports intra-monomer TPR-U-box
more » ... blocking the E2-binding surface of the U-box. We also discovered that monomers first dimerize symmetrically through their HH domains, which then triggers U-box dimerization. This brings the extended helices into close proximity, including a repulsive stretch of positively charged residues. Unable to smoothly unwind, this conflict bends the helices until the helix of one protomer breaks to relieve the repulsion. The abrupt snapping of the helix forces the C-terminal residues of the other protomer to disrupt that protomer's TPR-U-box tight binding interface, swiftly exposing and activating one of the E2 binding sites. Mutagenesis and biochemical experiments confirm that C-terminal residues are necessary both to maintain CHIP stability and function. This novel mechanism indicates how a ubiquitin ligase maintains an inactive monomeric form that rapidly activates only after asymmetric assembly. Generally, the lowest energy state of protein assembly is symmetrical, whereas asymmetry is associated with energy frustration and structural instability 1 . However, symmetry is not evolutionarily constrained, and many oligomeric enzymes are known to be asymmetric with only half-of-sites active 2-5 . In the majority of such oligomers, asymmetry results from conformational changes triggered by ligand binding 2, 6, 7 . Another mechanism to fold and activate asymmetric dimers requires that one of the ligand-binding sites is deformed 3, 6, 7 . However, very few of the vast number of known homo-multimeric proteins assemble into asymmetric structures 6 . Among the exceptions are the C-terminus of Hsc/p70-Interacting Protein (CHIP) 8 , an E3 ubiquitin ligase that associates with cyoplasmic Hsp70 and Hsp90 chaperones, and Hikeshi 9 , a nuclear import protein that also binds Hsp70. Unveiling the mechanism of symmetry breaking in homo-oligomers will shed light on new principles of folding and assembly for this important class of proteins. The CHIP homodimer consists of three domains: tetratricopeptide repeat (TPR), helix-turn-helix (HH), and U-box 8 (Fig. 1) . CHIP targets misfolded, chaperone-bound substrates for proteasomal degradation by transferring ubiquitin from a compatible E2 ubiquitin conjugating-enzyme, which associates with the U-box domain, to a lysine residue on the target protein 10, 11 . The crystal structure of murine CHIP (CHIP, PDB: 2C2L), which differs from human CHIP by one residue at the N-terminus, showcases an asymmetric homodimer in which only one U-box domain has an accessible E2-binding surface. The E2 binding site/U-box in the second protomer is blocked by intra-protomer packing against the TPR domain 8 (Fig. 1) . In this second protomer, the HH domain is bent, whereas the HH domain of the first protomer is extended. This pronounced asymmetry contrasts strongly with the symmetric structure of a Zebrafish CHIP construct (fCHIP, PDB: 2F42) from which the TPR domain is deleted 12 (Fig. 1) . Published: xx xx xxxx OPEN
doi:10.1038/s41598-017-01880-4 pmid:28496195 pmcid:PMC5431976 fatcat:qtbcnb2x5jbndmq46ecgtvn4ku

Dietary Inflammatory Index and the Risk of Hyperuricemia: A Cross-Sectional Study in Chinese Adult Residents

Chen Ye, Xiaojie Huang, Ruoyu Wang, Mairepaiti Halimulati, Sumiya Aihemaitijiang, Zhaofeng Zhang
2021 Nutrients  
Dietary Inflammatory Index (DII) scores have been consistently associated with several chronic diseases. This study explored the correlation between the DII and hyperuricemia in Chinese adult residents. Methods: The study included 7880 participants from the China Health and Nutrition Survey (CHNS), which was taken in in 2009. A 3-day 24 h meal review method was used to collect diet data and to calculate the DII score. Serum uric acid was obtained to determine hyperuricemia levels. Subjects were
more » ... divided into a hyperuricemia group and a non-hyperuricemia group, according to their serum uric acid level. Multilevel logistic regression models were used to examine the association between DII scores and hyperuricemia. Results: After adjusting for covariates, a higher DII score was determined to be associated with a higher risk of hyperuricemia. Compared to those in the highest DII score group, the lower DII score group had an inverse association with hyperuricemia risk (Q2: 0.83, 95% CI: 0.70–0.99; Q3: 0.72, 95% CI: 0.60–0.86; Q4: 0.73, 95% CI: 0.61–0.88). The intake of energy-adjusted protein, total fat, MUFAs, PUFAs and saturated fatty acid was higher in the hyperuricemia group. Conclusions: A higher DII score is significantly associated with a higher risk of hyperuricemia. Controlling the intake of pro-inflammatory food may be beneficial to reduce the risk of hyperuricemia.
doi:10.3390/nu13124504 pmid:34960057 pmcid:PMC8708184 fatcat:qelikywyjbgtvg3jems6s6yggq

ScaffComb: A Phenotype‐Based Framework for Drug Combination Virtual Screening in Large‐Scale Chemical Datasets

Zhaofeng Ye, Fengling Chen, Jiangyang Zeng, Juntao Gao, Michael Q. Zhang
2021 Advanced Science  
Combinational therapy is used for a long time in cancer treatment to overcome drug resistance related to monotherapy. Increased pharmacological data and the rapid development of deep learning methods have enabled the construction of models to predict and screen drug pairs. However, the size of drug libraries is restricted to hundreds to thousands of compounds. The ScaffComb framework, which aims to bridge the gaps in the virtual screening of drug combinations in large-scale databases, is
more » ... d here. Inspired by phenotype-based drug design, ScaffComb integrates phenotypic information into molecular scaffolds, which can be used to screen the drug library and identify potent drug combinations. First, ScaffComb is validated using the US food and drug administration dataset and known drug combinations are successfully reidentified. Then, ScaffComb is applied to screen the ZINC and ChEMBL databases, which yield novel drug combinations and reveal an ability to discover new synergistic mechanisms. To our knowledge, ScaffComb is the first method to use phenotype-based virtual screening of drug combinations in large-scale chemical datasets.
doi:10.1002/advs.202102092 pmid:34723439 pmcid:PMC8693048 fatcat:s6e33ucjsvhplcgane5mob7asi

Honokiol induces paraptosis-like cell death of acute promyelocytic leukemia via mTOR & MAPK signaling pathways activation

Xiaoli Liu, Yan Gu, Yaoyao Bian, Danhong Cai, Yu Li, Ye Zhao, Zhaofeng Zhang, Mei Xue, Liang Zhang
2021 Apoptosis  
Acute promyelocytic leukemia (APL) is a blood system disease caused by the accumulation of a large number of immature blood cells in bone marrow. Although the introduction of all-trans retinoic acid (ATRA) and arsenic has reached a high level of complete remission rate and 5-year disease-free survival rate, the occurrence of various adverse reactions still severely affects the quality of life of patients. As a natural product, honokiol (HNK) has the advantages of low toxicity and high
more » ... , and it is a potential drug for the treatment of cancer. Since cancer cells can escape apoptotic cell death through multiple adaptive mechanisms, HNK, a drug that induces cancer cell death in a nonapoptotic way, has attracted much interest. We found that HNK reduced the viability of human APL cell line (NB4 cells) by inducing paraptosis-like cell death. The process was accompanied by excessive reactive oxygen species (ROS), mitochondrial damage, endoplasmic reticulum stress, and increased microtubule-associated protein 1 light chain 3 (LC3) processing. The inactivation of proteasome activity was the main cause of misfolded and unfolded protein accumulation in endoplasmic reticulum, such as LC3II/I and p62. This phenomenon could be alleviated by adding cycloheximide (CHX), a protein synthesis inhibitor. We found that mTOR signaling pathway participated in paraptosis-like cell death induced by HNK in an autophagy-independent process. Moreover, the mitogen-activated protein kinase (MAPK) signaling pathway induced paraptosis of NB4 cells by promoting endoplasmic reticulum stress. In summary, these findings indicate that paraptosis may be a new way to treat APL, and provide novel insights into the potential mechanism of paraptosis-like cell death.
doi:10.1007/s10495-020-01655-9 pmid:33550458 fatcat:iuflyy7ryza75pa56n7lnppvxe

Microbially Mediated Methylation of Arsenic in the Arsenic-Rich Soils and Sediments of Jianghan Plain

Xian-Chun Zeng, Ye Yang, Wanxia Shi, Zhaofeng Peng, Xiaoming Chen, Xianbin Zhu, Yanxin Wang
2018 Frontiers in Microbiology  
., 2006; Ye et al., 2012; Yang and Rosen, 2016) , we proposed a conceptual model for the microbes-catalyzed methylation of As in the arsenic-rich sediments (Figure 7) .  ... 
doi:10.3389/fmicb.2018.01389 pmid:30034374 pmcid:PMC6043643 fatcat:gredwmh5mvclzhnhzwqeac6wwe

Predicting protein targets for drug-like compounds using transcriptomics [article]

Nicolas A Pabon, Yan Xia, Samuel K. Estabrooks, Zhaofeng Ye, Amanda K. Herbrand, Evelyn Sub, Ricardo M. Biondi, Victoria A. Assimon, Jason E. Gestwicki, Jeffrey L. Brodsky, Carlos J. Camacho, Ziv Bar-Joseph
2018 bioRxiv   pre-print
The development of an expanded chemical space for screening is an essential step in the challenge of identifying chemical probes for new, genomic-era protein targets. However, the difficulty of identifying targets for novel compounds leads to the prioritization of synthesis linked to known active scaffolds that bind familiar protein families, slowing the exploration of available chemical space. To change this paradigm, we validated a new pipeline capable of identifying compound-protein
more » ... ons even for compounds with no similarity to known drugs. Based on differential mRNA profiles from drug treatments and gene knockdowns across multiple cell types, we show that drugs cause gene regulatory network effects that correlate with those produced by silencing their target protein-coding gene. Applying supervised machine learning to exploit compound-knockdown signature correlations and enriching our predictions using an orthogonal structure-based screen, we achieved top-10/top-100 target prediction accuracies of 26%/41%, respectively, on a validation set 152 FDA-approved drugs and 3104 potential targets. We further predicted targets for 1680 compounds and validated a total of seven novel interactions with four difficult targets, including non-covalent modulators of HRAS and KRAS. We found that drug-target interactions manifest as gene expression correlations between drug treatment and both target gene knockdown and up/down-stream knockdowns. These correlations provide biologically relevant insight on the cell-level impact of disrupting protein interactions, highlighting the complex genetic phenotypes of drug treatments. Our pipeline can accelerate the identification and development of novel chemistries with potential to become drugs by screening for compound-target interactions in the full human interactome.
doi:10.1101/254367 fatcat:52ovirk3j5cflg54c4z6lcrsau

Effects of grape seed proanthocyanidin extract on renal injury in type 2 diabetic rats

2014 Molecular Medicine Reports  
Grape seed proanthocyanidin extract (GSPE) is known to be an effective natural polyphenol capable of removing free radicals in vivo. It has been reported that GSPE has biological functions including antioxidant, anti-cancer, anti-hyperglycemic, anti-radiation, and prevention and treatment of cardiovascular diseases. This study aims to investigate the effects of GSPE on renal injury in type 2 diabetic rats induced with low-dose streptozotocin and a high-carbohydrate/high-fat diet. Rats (n=12 per
more » ... group) were administered GSPE at either a low (125 mg/kg·bw), medium (250 mg/kg·bw) or high (500 mg/kg·bw) dose, while control rats and diabetes mellitus group rats received no specific treatment. After 16 weeks, GSPE slightly increased body weight and decreased food consumption, water intake and urine volume in rats. Diabetic rats treated with GSPE demonstrated decreased fasting blood glucose, serum insulin, HbA1c and systolic blood pressure (P<0.05). GSPE significantly improved renal function parameters, reduced the expression of tissue inhibitor of metalloproteinase-1 and also increased the activity of matrix metalloproteinase-9. Moreover, GSPE (particularly at a dose of 500 mg/kg·bw) increased the activity of antioxidant enzymes and reduced the levels of c-reactive proteins (P<0.01) in serum and the expression of tumor necrosis factor-α, monocyte chemoattractant protein-1 and intercellular adhesion molecule-1 (P<0.05) in the kidney. These findings suggest that GSPE ameliorates renal injury in type 2 diabetic rats through its antioxidative activity and anti-inflammatory effects. Abbreviations: FBG, fasting blood glucose; hs-CRP, high-sensitivity c-reactive protein; SCr, serum creatinine; CCr, endogenous creatinine clearance rate; BUN, blood urea nitrogen; SOD, superoxide dismutase; CAT, catalase; MDA, malondialdehyde; TNF-α, tumor necrosis factor-α; TIMP-1, tissue inhibitor of metalloproteinase-1; ICAM-1, intercellular adhesion molecule-1; MCP-1, monocyte chemoattractant protein-1; MMP-9, matrix metalloproteinase-9
doi:10.3892/mmr.2014.2768 pmid:25351255 fatcat:sdxzmjgtjrax5haz4govijm3dm

An ultra-wideband injection-locked frequency divider with a multi-order resonator and the passive injection-boosted technique

Shuai Cheng, Hongbo Ye, Linhong Li, Zhaofeng Zhang
2022 IEICE Electronics Express  
This paper presents an ultra-wideband injection-locked frequency divider (ILFD) design which is applicable to a multi-applications system. A transformer-based multi-order resonator is utilized in the ILFD to attain a flat phase response. The passive injection-boosted technique is proposed to increase the injection signal amplitude and thus widen the locking range. With the injection-boosted technique and the multi-order resonator, the proposed ILFD demonstrates a measured locking range of 50
more » ... , covering 48 GHz to 98 GHz. The divider designed in a 40-nm process consumes a DC power of 11.2 mW excluding buffers and occupies a core size of 0.38 × 0.63 mm 2 .
doi:10.1587/elex.19.20220108 fatcat:ia63v3llkvhbjjglmadyn4pghu

Porous SiC/melamine-derived carbon foam frameworks with excellent electromagnetic wave absorbing capacity

Xinli Ye, Zhaofeng Chen, Sufen Ai, Bin Hou, Junxiong Zhang, Xiaohui Liang, Qianbo Zhou, Hezhou Liu, Sheng Cui
2019 Journal of Advanced Ceramics  
Porous three-dimensional SiC/melamine-derived carbon foam (3D-SiC/MDCF) composite with an original open pore structure was fabricated by the heat treatment of the commercial melamine foam (MF), carbonization of the stable MF, and chemical vapor deposition of the ultra-thin SiC coating. Scanning electron microscopy (SEM) and X-ray diffraction (XRD) were employed to detect the microstructure and morphology of the as-prepared composites. The results indicated that the 3D-SiC/MDCF composites with
more » ... e coating structure were prepared successfully. The obtained minimum reflection loss was -29.50 dB when the frequency and absorption thickness were 11.36 GHz and 1.75 mm, respectively. Further, a novel strategy was put forward to state that the best microwave absorption property with a thin thickness of 1.65 mm was gained, where the minimum reflection loss was -24.51 dB and the frequency bandwidth was 3.08 GHz. The excellent electromagnetic wave absorption ability resulted from the specific cladding structure, which could change the raw dielectric property to acquire excellent impedance matching. This present work had a certain extend reference meaning for the potential applications of the lightweight wave absorption materials with target functionalities.
doi:10.1007/s40145-019-0328-2 fatcat:j66pak2jyjh2npijat6k7qhggu
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