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Rei Kawashima, Yuki I. Kawamura, Tomoyuki Oshio, Noriko Mizutani, Toshihiko Okada, Yutaka J. Kawamura, Fumio Konishi, Taeko Dohi
2012 Journal of immunoassay & immunochemistry  
TABLE 1 1 Patient Demographics Case Age Sex Operation Stage Operation Time (h) 1 51 Male Intersphincteric resection with loop ileostomy I 5.00 2 74 Female Sigmoid colectomy II 2.25 3  ... 
doi:10.1080/15321819.2011.638409 pmid:22738652 pmcid:PMC3431549 fatcat:lplma4rruvaovbnmkmnjiyfibq

Profiling of tumour-associated microbiota in human hepatocellular carcinoma

Seiga Komiyama, Takahiro Yamada, Nobuyuki Takemura, Norihiro Kokudo, Koji Hase, Yuki I. Kawamura
2021 Scientific Reports  
(h,i) Discriminating taxa between the non-tumour and tumour regions of primary (h) or metastatic liver cancers (i) determined by LefSe analysis 15 .  ... 
doi:10.1038/s41598-021-89963-1 pmid:34012007 fatcat:olrvu4hmibadlkmhzsc7vjk7yu

Disruption of the TWEAK/Fn14 pathway prevents 5-fluorouracil-induced diarrhea in mice

Takuhito Sezaki, Yuki Hirata, Teruki Hagiwara, Yuki I Kawamura, Tadashi Okamura, Rieko Takanashi, Kenta Nakano, Miwa Tamura-Nakano, Linda C Burkly, Taeko Dohi
2017 World Journal of Gastroenterology  
AIM To clarify the roles of TWEAK and its receptor Fn14 in 5-fluorouracil (5-FU)-induced diarrhea. METHODS Diarrhea was induced in wild-type (WT), Fn14 knockout (KO), and IL-13 receptor (IL-13R)α1 KO BALB/c mice using a single injection of 5-FU. Histological analysis, cytokine analysis, and flow cytometry was performed on ileal tissues and cells. Murine colon carcinomabearing mice were co-treated with an anti-TWEAK antibody and 5-FU. Embryonic fibroblast response to cytokines was also analyzed.
more » ... RESULTS 5-FU induced high Fn14 expression in epithelial cells. The severity of 5-FU-induced diarrhea was lower in Fn14 KO mice compared with WT mice. Administration of anti-TWEAK antibody reduced 5-FU-induced diarrhea without affecting the antitumor effects of 5-FU in vivo . 5-FU-induced expression of IL-13, IL-17A, TNF-α, and IFN-γ in the ileum was Fn14 dependent. The severity of 5-FU-induced diarrhea was lower in IL-13Rα1 KO mice, indicating major role for IL-13 signaling via IL-13Rα1 in pathogenesis. We found that IL-13Rα2, an IL-13 neutralizing/cell protective receptor, was strongly induced by IL-33 in vitro and in vivo . was upregulated in the ileum of 5-FU-treated Fn14 KO mice. Thus, the deletion of Fn14 upregulated IL-13Rα2 expression, which reduced IL-13 expression and activity. CONCLUSION Disruption of the TWEAK/Fn14 pathway affects several interconnected pathways, including those associated with IL-13, IL-33, and IL-13Rα2, to attenuate 5-FUinduced intestinal side effects.
doi:10.3748/wjg.v23.i13.2294 pmid:28428709 pmcid:PMC5385396 fatcat:7cpbsnpxwvd4ldys5ebsf5kc2m

Novel protocol to observe the intestinal tuft cell using transmission electron microscopy

Takuma Kozono, Miwa Tamura-Nakano, Yuki I. Kawamura, Takashi Tonozuka, Atsushi Nishikawa
2022 Biology Open  
Each area surrounded with box in the G are shown as magnified images in H and I. (H,I) The yellow arrowheads show Golgi apparatus (H) and multivesicular bodies (I).  ...  Each area surrounded with box in the image of (H,I) are shown as magnified images in the right (H) or below (I). The white arrows in (B-D,I) show the filamentous structures.  ... 
doi:10.1242/bio.059007 pmid:34994390 pmcid:PMC8864298 fatcat:g2dnhqnf2bendenjxunss3aaw4

CD4+CD45RBHi Interleukin-4 Defective T Cells Elicit Antral Gastritis and Duodenitis

Taeko Dohi, Kohtaro Fujihashi, Toshiya Koga, Yuri Etani, Naoto Yoshino, Yuki I. Kawamura, Jerry R. McGhee
2004 American Journal of Pathology  
I: Peyer's patches of TCR Ϫ/Ϫ mice without T cell transfer.  ...  I: The fundic lesion with mild changes was taken from the same specimen as shown in G.  ... 
doi:10.1016/s0002-9440(10)63385-8 pmid:15466391 pmcid:PMC1618629 fatcat:os6hkya6ojfz5h3ejewouwl4yu

Introduction of SdaCarbohydrate Antigen in Gastrointestinal Cancer Cells Eliminates Selectin Ligands and Inhibits Metastasis

Yuki I. Kawamura, Rei Kawashima, Ryuko Fukunaga, Kazunari Hirai, Noriko Toyama-Sorimachi, Makoto Tokuhara, Toshio Shimizu, Taeko Dohi
2005 Cancer Research  
Kawamura for his helpful advice and discussion.  ... 
doi:10.1158/0008-5472.can-05-0639 pmid:16024623 fatcat:phxnjj3r6jaf3m5bcg7b6xxxza

The monoclonal antibody HCM31 specifically recognises the Sda tetrasaccharide in goblet cell mucin

Daigo Tsubokawa, Yukinobu Goso, Rei Kawashima, Hiroyoshi Ota, Takeshi Nakamura, Kazuo Nakamura, Noriko Sato, Makoto Kurihara, Taeko Dohi, Yuki I. Kawamura, Takafumi Ichikawa, Kazuhiko Ishihara
2012 FEBS Open Bio  
I. Kawashima and K. Ogura for kindly providing us with the anti-GM2 antibody.  ...  Kawamura et al. [19] reported that metastasis of human gastrointestinal cancer cells was reduced by expression of the Sd a antigen on their surface.  ...  The fragmentation spectrum of IA1-6 ( m/z 1427 as [M+Na] + ) included the series of B i ions at m/z 591, 693 and 896 and Y i ion at m/z 916 (data not shown), which indicated the presence of the sequence  ... 
doi:10.1016/j.fob.2012.07.006 pmid:23650604 pmcid:PMC3642157 fatcat:x7tkhd5epngezo2yekycxejpey

Intermittent fasting prompted recovery from dextran sulfate sodium-induced colitis in mice

Toshihiko Okada, Takeshi Otsubo, Teruki Hagiwara, Fumika Inazuka, Eiko Kobayashi, Shinji Fukuda, Takuya Inoue, Kazuhide Higuchi, Yuki I. Kawamura, Taeko Dohi
2017 Journal of clinical biochemistry and nutrition  
After the RNA was treated with DNase I, double-stranded cDNA was synthesized using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA).  ... 
doi:10.3164/jcbn.17-9 pmid:28955126 pmcid:PMC5612824 fatcat:5xc6qeb3yfak7ncdi3rstjv6yq

Clinicopathological significance of cystatin A expression in progression of esophageal squamous cell carcinoma

Daiki Shiba, Masayoshi Terayama, Kazuhiko Yamada, Teruki Hagiwara, Chinatsu Oyama, Miwa Tamura-Nakano, Toru Igari, Chizu Yokoi, Daisuke Soma, Kyoko Nohara, Satoshi Yamashita, Taeko Dohi (+1 others)
2018 Medicine  
Number of patients (%) Writingoriginal draft: Daiki Shiba, Masayoshi Terayama, Taeko Dohi, Yuki I. Kawamura.  ...  I.  ... 
doi:10.1097/md.0000000000010357 pmid:29642180 pmcid:PMC5908574 fatcat:yr2smbyng5bdlhvqvpqbykurea

Aberrant DNA hypermethylation reduces the expression of the desmosome-related molecule periplakin in esophageal squamous cell carcinoma

Takeshi Otsubo, Teruki Hagiwara, Miwa Tamura-Nakano, Takuhito Sezaki, Oki Miyake, Chihaya Hinohara, Toshio Shimizu, Kazuhiko Yamada, Taeko Dohi, Yuki I. Kawamura
2015 Cancer Medicine  
After the RNA was treated with DNase I, double-stranded cDNA was synthesized using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA).  ...  ester (Dojindo, Kumamoto, Japan), and suspended in Dulbecco's modified Eagle's medium with 0.1% bovine serum albumin at the density of 2 9 10 5 /well of 24-well tissue culture plates coated with type I  ... 
doi:10.1002/cam4.369 pmid:25583674 pmcid:PMC4380967 fatcat:mqyyf6dufjawnmcfdtcgoqzhq4

A Histone Methyltransferase ESET Is Critical for T Cell Development

Shoichi Takikita, Ryunosuke Muro, Toshiyuki Takai, Takeshi Otsubo, Yuki I. Kawamura, Taeko Dohi, Hiroyo Oda, Masayuki Kitajima, Kenshiro Oshima, Masahira Hattori, Takaho A. Endo, Tetsuro Toyoda (+3 others)
2016 Journal of Immunology  
At the same time, differentiation of class I-restricted OT-I TCR CD8-SP was strongly inhibited (Fig. 2B) .  ...  For FACS, Abs listed in the Supplemental Table I were used.  ... 
doi:10.4049/jimmunol.1502486 pmid:27511731 fatcat:r3tildiprvahnb3sspsbnhswme

Retrotransposition of Long Interspersed Nucleotide Element-1 Is Associated with Colitis but Not Tumors in a Murine Colitic Cancer Model

Takeshi Otsubo, Tadashi Okamura, Teruki Hagiwara, Yukihito Ishizaka, Taeko Dohi, Yuki I. Kawamura, Emiko Mizoguchi
2015 PLoS ONE  
Long interspersed element-1 (L1) is a transposable element that can move within the genome, potentially leading to genome diversity and modified gene function. Although L1 activity in somatic cells is normally suppressed through DNA methylation, some L1s are activated in tumors including colorectal carcinoma. However, how L1-retrotransposition (L1-RTP) is involved in gastrointestinal disorders remains to be elucidated. We hypothesized that L1-RTP in somatic cells might contribute to
more » ... ciated cancer (CAC). To address this, we employed an experimental model of CAC using transgenic L1-reporter mice carrying a human L1-EGFP reporter gene. Mice were subjected to repeated cycles of colitis induced by administration of dextran sodium sulfate (DSS) in drinking water with injection of carcinogen azoxymethane (AOM). L1-RTP levels were measured by a quantitative polymerase chain reaction targeting the newly inserted reporter EGFP in various tissues and cell types, including samples obtained by laser microdissection and cell sorting with flow cytometry. DNA methylation levels of the human L1 promoter were analyzed by bisulfite pyrosequencing. AOM+DSS-treated mice exhibited significantly higher levels of L1-RTP in whole colon tissue during the acute phase of colitis when compared with control naïve mice. L1-RTP levels in whole colon tissue were positively correlated with the histological severity of colitis and degree of neutrophil infiltration into the lamina propria (LP), but not with tumor development in the colon. L1-RTP was enriched in LP mesenchymal cells rather than epithelial cells (ECs), myeloid, or lymphoid cells. DNA methylation levels of the human L1 promoter region showed a negative correlation with L1-RTP levels. L1-RTP was absent from most tumors found in 22-week-old mice. In conclusion, we demonstrated that L1-RTP was induced in the mouse CAC mucosa in accordance with the acute inflammatory PLOS ONE |
doi:10.1371/journal.pone.0116072 pmid:25710700 pmcid:PMC4339839 fatcat:q4ut6whsbvcmvcfmeejmztjq7y

DNA hypermethyation and silencing of PITX1 correlated with advanced stage and poor postoperative prognosis of esophageal squamous cell carcinoma

Takeshi Otsubo, Kazuhiko Yamada, Teruki Hagiwara, Kenshiro Oshima, Kei Iida, Koro Nishikata, Tetsuro Toyoda, Toru Igari, Kyoko Nohara, Satoshi Yamashita, Masahira Hattori, Taeko Dohi (+1 others)
2017 OncoTarget  
Briefly, total RNA was treated with DNase I, and then bound to Dynabeads Oligo(dT) EcoP magnetic beads for cDNA synthesis.  ...  After the RNA was treated with DNase I, doublestranded cDNA was synthesized using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA).  ... 
doi:10.18632/oncotarget.21375 pmid:29137437 pmcid:PMC5663609 fatcat:ab6qlxbxxffvrdsebtwgeeirae

Loss of GSTO2 contributes to cell growth and mitochondria function via the p38 signaling in lung squamous cell carcinoma

Ryusuke Sumiya, Masayoshi Terayama, Teruki Hagiwara, Kazuaki Nakata, Keigo Sekihara, Satoshi Nagasaka, Hideki Miyazaki, Toru Igari, Kazuhiko Yamada, Yuki I. Kawamura
2021 Cancer Science  
*P < .05, **P < .01, ***P < .001 F I G U R E 5 Role of GSTO2 in normal lung and LSCC cells.  ...  F I G U R E 4 4 Ectopic expression of GSTO2 inhibited mitochondrial function through p38 phosphorylation.  ...  CO N FLI C T O F I NTE R E S T The authors have no conflicts of interest to declare. O RCI D Ryusuke Sumiya  ... 
doi:10.1111/cas.15189 pmid:34726807 pmcid:PMC8748250 fatcat:q7u2waypjbe7ldhjm5phuzikea

Chemokine Receptor CCR8 Is Required for Lipopolysaccharide-Triggered Cytokine Production in Mouse Peritoneal Macrophages

Tomoyuki Oshio, Rei Kawashima, Yuki I. Kawamura, Teruki Hagiwara, Noriko Mizutani, Toshihiko Okada, Takeshi Otsubo, Kyoko Inagaki-Ohara, Akihiro Matsukawa, Tatsuya Haga, Shigeru Kakuta, Yoichiro Iwakura (+3 others)
2014 PLoS ONE  
One member of the C-C motif chemokine superfamily, CCL1/I-309, binds to the chemokine receptor CCR8 and induces Ca 2+ influx and monocyte migration in humans [3] .  ... 
doi:10.1371/journal.pone.0094445 pmid:24714157 pmcid:PMC3979852 fatcat:ne5pb7afanfdzifdlepb5f53xe
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