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12β-Acetyloxyperforatin, a New Limonoid from Harrisonia Perforata

Peng-Yan Gui, Xiao-Hui Yan, Wen-Juan Yuan, Yuan-Liang Ma, Xiao Ding, Shuai Liu, Ying-Tong Di, Wei-Ming Zhu, Xiao-Jiang Hao
2018 Figshare  
A new A, D-seco limonoid, named 12-acetyloxyperforatin (1), along with three known ones, were isolated from the leaves of Harrisonia perforata. Their structures were elucidated on the basis of spectroscopic analysis, including extensive NMR techniques and computational modelling. These compounds showed no inhibitory activity against the 11β-HSD1 enzyme.
doi:10.6084/m9.figshare.7294889.v1 fatcat:ml3wswdk7bhylly4fcwlhdv5rm

Spatial organization of heterologous metabolic system in vivo based on TALE

Lv-yun Zhu, Xin-yuan Qiu, Ling-yun Zhu, Xiao-min Wu, Yuan Zhang, Qian-hui Zhu, Dong-yu Fan, Chu-shu Zhu, Dong-yi Zhang
2016 Scientific Reports  
Spatial organization of heterologous metabolic system in vivo based on TALE Lv-yun Zhu, Xin-yuan Qiu, Ling-yun Zhu, Xiao-min Wu, Yuan Zhang, Qian-hui Zhu, Dong-yu Fan, Chu-shu Zhu & Dong-yi Zhang Scientific  ...  The original HTML version of this Article contained typographical errors in the spelling of the author Lv-yun Zhu which was incorrectly given as Ling-yun Zhu.  ... 
doi:10.1038/srep26065 pmid:27184291 pmcid:PMC4869064 fatcat:yze6oh273nepxdiutvkofvqiwi

Chlormethine Hydrochloride is Not Inferior to Tacrolimus in Treating Steroid-Resistant Nephrotic Syndrome

Yuan Yang, Li Zhao, Li Xiao, Yumei Liang, Chang Wang, Xiao Fu, Xuejing Zhu, Shuguang Yuan, Jianling Zhu, Xiaoping Zhu, Yinghong Liu, Jun Li (+3 others)
2018 Kidney & Blood Pressure Research  
Yuan Y, Li Zh and Lin S were responsible for the drafting and revision of the manuscript.  ... 
doi:10.1159/000486911 pmid:29402844 fatcat:qtmurxgbhrbfxcikksfj2hnrri

SNARE proteins SYP22 and VAMP727 negatively regulate plant defense

Xiao Feng Zhu, Yang Liu, Xiao Tong Gai, Yuan Zhou, Zhen Yuan Xia, Li Jie Chen, Yu Xi Duan, Yuan Hu Xuan
2019 Plant Signalling & Behavior  
CONTACT Yuan Hu Xuan, College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China *These authors are co-fist authors and contributed equally to this  ... 
doi:10.1080/15592324.2019.1610300 pmid:31025584 pmcid:PMC6619965 fatcat:c65ltayx7bgsdajs43fvnqqbbm

Vaspin regulates the osteogenic differentiation of MC3T3-E1 through the PI3K-Akt/miR-34c loop

Yuan Liu, Feng Xu, Hong-Xia Pei, Xiao Zhu, Xiao Lin, Cheng-Yuan Song, Qiu-Hua Liang, Er-Yuan Liao, Ling-Qing Yuan
2016 Scientific Reports  
Vaspin (visceral adipose tissue-derived serine protease inhibitor) is a newly discovered adipokine that widely participates in diabetes mellitus, polycystic ovarian syndrome and other disorders of metabolism. However, the effect of vaspin on the regulation of osteogenesis and the mechanism responsible are still unclear. Here, we found that vaspin can attenuate the osteogenic differentiation of the preosteoblast cell line MC3T3-E1 in a dose-dependent way; also, during this process, the
more » ... of miRNA-34c (miR-34c) was significantly increased. Down-regulation of the expression of miR-34c in MC3T3-E1 diminished the osteogenic inhibitory effect of vaspin, while the up-regulation of miR-34c increased this effect through its target gene Runx2. Meanwhile, we found that vaspin could also activate the PI3K-Akt signalling pathway. Blocking the PI3K-Akt signalling pathway with specific inhibitors could decrease the osteogenic inhibitory effect of vaspin as well as the expression level of miR-34c. Furthermore, knock-down of miR-34c could promote the activation of Akt, which was probably realised by targeting c-met expression. Thus, PI3K-Akt and miR-34c constituted a modulation loop and controlled the expression of each other. Taken together, our study showed that vaspin could inhibit the osteogenic differentiation in vitro, and the PI3K-Akt/miR-34c loop might be the underlying mechanism. Fat is the largest endocrine organ in the human body and has a complex connection with bone. In the past, it was widely accepted that fat was a protective factor for bone mass. The loading effect caused by fat mass cooperates with that of muscle mass and can protect the bone mineral density (BMD) from declining 1 . However, recent studies have challenged the conventional standpoint which states that fat might be a beneficial factor for bone health. One study carried out in 197 elderly patients showed that fracture risk was positively related to the fat mass in the bone marrow 2 , and this viewpoint gained support from additional researches. According to these studies, negative associations between fat mass and bone tissue have been demonstrated in children, men, and pre-and post-menopausal women 3-9 . Recently, visceral adipose tissue (VAT) has gained increasing attention on the basis of robust evidence linking VAT and insulin resistance, metabolic syndrome, dyslipidaemia, cardiovascular disease, hypertension, and cancer 10-14 . In addition, according to Cohen's study, VAT has been shown to show the most significant association with lower BMD; post-menopausal women with more VAT showed a significant decline in both BMD and microarchitecture, and also the bone regeneration rate was greatly suppressed 15 . Furthermore, the similar result was found in Júnior's study, who found that BMD was negatively correlated with VAT 16 . These results all indicated that VAT might have a negative effect on bone, and the adipokines might be responsible. To date, many adipokines have been found to participate in bone metabolism. Most of them act as promoting factors on osteogenic differentiation, for instance, adiponectin 17 , visfatin 18 , omentin-1 19,20 and resistin 21 . However, the effect of vaspin on osteogenic differentiation still needs to be illuminated. Vaspin is a secretory protein which is mainly synthesised in the visceral fat tissue; however, its expression has also been reported in the liver, pancreas, and skin 22,23 . The expression of vaspin could be affected by many
doi:10.1038/srep25578 pmid:27156573 pmcid:PMC4860647 fatcat:5yihwkoodrhdjpdp2bjo4z6a7e

A novel process of ozone catalytic oxidation for low concentration formaldehyde removal

Bin Zhu, Xiao-Song Li, Peng Sun, Jing-Lin Liu, Xiao-Yuan Ma, Xiaobing Zhu, Ai-Min Zhu
2017 Chinese Journal of Catalysis  
To reduce energy costs, minimize secondary pollution from undecomposed ozone, and improve the efficiency of ozone use, a novel process of cycled storage-ozone catalytic oxidation (OZCO) was employed to remove formaldehyde (HCHO) at low concentrations in air. We applied Al2O3-supported manganese oxide (MnOx) catalysts to this process, and examined the HCHO adsorption capacity and OZCO performance over the MnOx catalysts. Owing to the high dispersion of MnOx and low oxidation state of manganese,
more » ... he MnOx/Al2O3 catalysts with a manganese acetate precursor and 10%-Mn loading showed good performance in both storage and OZCO stages. The presence of H2O led to a decrease of the HCHO adsorption capacity owing to competitive adsorption between moisture and HCHO at the storage stage; however, high relative humidity (RH) favored complete conversion of stored HCHO to CO2 at the OZCO stage and contributed to an excellent carbon balance. Four low concentration HCHO storage-OZCO cycles with a long HCHO storage period and relatively short OZCO period were successfully performed over the selected MnOx/Al2O3 catalyst at room temperature and a RH of 50%, demonstrating that the proposed storage-OZCO process is an economical, reliable, and promising technique for indoor air purification.
doi:10.1016/s1872-2067(17)62890-0 fatcat:azdlepyd3zbyldsndajjd3dwdi

Comparative study on the diagnostic values of different ultrasound technologies for malignant thyroid nodules

Xiao-Zhu Jin, Wei-Wei Lu, Hai-Feng Zhang, Yuan-Yuan Yan, Xiao-Le Gu
2018 Oncology Letters  
The present study compared and analyzed the diagnostic values of B-mode ultrasound and elasticity imaging technology for the identification of benign and malignant thyroid nodules. Ninety-four patients who were diagnosed with thyroid nodules by ultrasound were chosen. All patients were checked with B-mode ultrasound and ultrasound elasticity imaging technology before surgeries. Further, the post-operative outcomes were compared with operation pathology. The pathological examination results were
more » ... taken as the gold standard. The accuracy, sensitivity, specificity, positive predictive value and negative predictive values of combined ultrasound diagnosis were all obviously higher than those by the B-mode ultrasound and the ultrasound elastography method (P<0.05). In conclusion, as a new technology, ultrasound elasticity imaging technology has relatively high diagnositic value in identifying benign and malignant thyroid nodules. With its own advantages and limitations, the ultrasound elasticity imaging technology could effectively complement B-mode ultrasound, and improve the sensitivity, specificity and accuracy of ultrasound diagnosis for thyroid nodules.
doi:10.3892/ol.2018.8706 pmid:29963163 pmcid:PMC6019883 fatcat:j2faqo2bdjgtjph52nx3mv73je

ATF4- and CHOP-Dependent Induction of FGF21 through Endoplasmic Reticulum Stress

Xiao-shan Wan, Xiang-hong Lu, Ye-cheng Xiao, Yuan Lin, Hong Zhu, Ting Ding, Ying Yang, Yan Huang, Yi Zhang, Yan-Long Liu, Zhu-mei Xu, Jian Xiao (+1 others)
2014 BioMed Research International  
Fibroblast growth factor 21 (FGF21) is an important endogenous regulator involved in the regulation of glucose and lipid metabolism. FGF21 expression is strongly induced in animal and human subjects with metabolic diseases, but little is known about the molecular mechanism. Endoplasmic reticulum (ER) stress plays an essential role in metabolic homeostasis and is observed in numerous pathological processes, including type 2 diabetes, overweight, nonalcoholic fatty liver disease (NAFLD). In this
more » ... tudy, we investigate the correlation between the expression of FGF21 and ER stress. We demonstrated that TG-induced ER stress directly regulated the expression and secretion of FGF21 in a dose- and time-dependent manner. FGF21 is the target gene for activating transcription factor 4 (ATF4) and CCAAT enhancer binding protein homologous protein (CHOP). Suppression of CHOP impaired the transcriptional activation of FGF21 by TG-induced ER stress in CHOP−/− mouse primary hepatocytes (MPH), and overexpression of ATF4 and CHOP resulted in FGF21 promoter activation to initiate the transcriptional programme. In mRNA stability assay, we indicated that ER stress increased the half-life of mRNA of FGF21 significantly. In conclusion, FGF21 expression is regulated by ER stress via ATF- and CHOP-dependent transcriptional mechanism and posttranscriptional mechanism, respectively.
doi:10.1155/2014/807874 pmid:24900988 pmcid:PMC4037570 fatcat:ks7t36ryy5espagaugpbu37jwy

Multiple Synostoses Syndrome Is Due to a Missense Mutation in Exon 2 of FGF9 Gene

Xiao-lin Wu, Ming-min Gu, Lei Huang, Xue-song Liu, Hong-xin Zhang, Xiao-yi Ding, Jian-qiang Xu, Bin Cui, Long Wang, Shun-yuan Lu, Xiao-yi Chen, Hai-guo Zhang (+8 others)
2009 American Journal of Human Genetics  
Fibroblast growth factors (FGFs) play diverse roles in several developmental processes. Mutations leading to deregulated FGF signaling can cause human skeletal dysplasias and cancer. 1,2 Here we report a missense mutation (Ser99Asp) in exon 2 of FGF9 in 12 patients with multiple synostoses syndrome (SYNS) in a large Chinese family. In vitro studies demonstrate that FGF9 S99N is expressed and secreted as efficiently as wild-type FGF9 in transfected cells. However, FGF9 S99N induces compromised
more » ... ondrocyte proliferation and differentiation, which is accompanied by enhanced osteogenic differentiation and matrix mineralization of bone marrow-derived mesenchymal stem cells (BMSCs). Biochemical analysis reveals that S99N mutation in FGF9 leads to significantly impaired FGF signaling, as evidenced by diminished activity of Erk1/2 pathway and decreased b-catenin and c-Myc expression when compared with wild-type FGF9. Importantly, the binding of FGF9 S99N to its receptor is severely impaired although the dimerization ability of mutant FGF9 itself or with wildtype FGF9 is not detectably affected, providing a basis for the defective FGFR signaling. Collectively, our data demonstrate a previously uncharacterized mutation in FGF9 as one of the causes of SYNS, implicating an important role of FGF9 in normal joint development.
doi:10.1016/j.ajhg.2009.06.007 pmid:19589401 pmcid:PMC2706969 fatcat:tdlgwvxo6zettbnwvcingzdz3u

The role of pineal microRNA-325 in regulating circadian rhythms after neonatal hypoxic-ischemic brain damage

Xing Feng, Xin Ding, Ning Sha, Hua-Wei Wang, Bin Sun, Min Gong, Po Miao, Xiao-Lu Jiang, Xiao-Feng Yang, Mei Li, Li-Xiao Xu, Chen-Xi Feng (+4 others)
2021 Neural Regeneration Research  
Jiang 3 , Xiao-Feng Yang 2 , Mei Li 3 , Li-Xiao Xu 3 , Chen-Xi Feng 3 , Yuan-Yuan Yang 4 , Jie Zhang 5 , Wen-Jing Zhu 5 , Yuan-Yuan Gao 6 , Xing Feng 2 , Xin Ding MD, PhD 2 1 Soochow Key Laboratory  ...  Neural Regen Res 2021;16:2071-7 How to cite this URL: Sha N, Wang HW, Sun B, Gong M, Miao P, Jiang XL, Yang XF, Li M, Xu LX, Feng CX, Yang YY, Zhang J, Zhu WJ, Gao YY, Feng X, Ding X.  ... 
doi:10.4103/1673-5374.308101 pmid:33642396 fatcat:ejqaneyk35dbfktilzq24f4tq4

An enantiopure FeIII4 single-molecule magnet

Yuan-Yuan Zhu, Xiao Guo, Chang Cui, Bing-Wu Wang, Zhe-Ming Wang, Song Gao
2011 Chemical Communications  
Physical measurement Magnetic measurements were performed on a Quantum Design MPMS XL-5 SQUID magnetometer on polycrystalline samples. Data were corrected for the diamagnetism of the samples using Pascal constants and of the sample holder by measurement. The experiments below 1.8 K were measured on the iHelium Measurement Console in coordination with MPMS MultiVu. The X-ray measurements of 1R were carried on a Saturn724+ CCD diffractometer with graphite-monochromator Mo-Kα radiation (λ =
more » ... Å) at 173 K. Intensities were collected using CrystalClear (Rigaku Inc., 2008) technique and absorption effects were collected using the Numerical technique. The X-ray measurements of 1S were carried on a Saturn724+ CCD diffractometer with Confocal-monochromator Mo-Kα radiation (λ = 0.71073 Å) at 173 K. Intensities were collected using CrystalClear (Rigaku Inc., 2008) technique and absorption effects were collected using the multi-scan technique. The structure of 1R and 1S was solved using SHELXS-97 program 1 and refined by a full matrix least squares technique based on F 2 using SHELXL 97 program. 2 CD spectral measurements in solution and solid state were performed on a JASCO J-815 CD spectropolarimeter. The CD spectra were calculated with the TDDFT method at the B3LYP/6-31G* level of theory for three model fragment structures of 1R. Hydrogen atoms were added theoretically to balance the charge. The standard Pople style basis sets, 6-31G with one set of 'd-' polarization functions on S1 Electronic Supplementary Material (ESI) for Chemical Communications This journal is
doi:10.1039/c1cc12831k pmid:21677989 fatcat:f7gbeqsy4faajc5gjjzx4s4zam

Carnosine, a precursor of histidine, ameliorates pentylenetetrazole-induced kindled seizures in rat

Xiao-hua Wu, Mei-ping Ding, Zheng-Bing Zhu-Ge, Yuan-Yuan Zhu, Chun-lei Jin, Zhong Chen
2006 Neuroscience Letters  
Carnosine (␤-alanyl-l-histidine) has been characterized as a putative neurotransmitter. However, so far, understanding of the role of carnosine in the brain is very limited. The objective of this study was to examine the effects of carnosine on the development of pentylenetetrazol (PTZ) kindling seizures and protection against the PTZ kindled seizures in rats. Chemical kindling was elicited by repeated intraperitoneal injection of PTZ (35 mg/kg) once every 48 h until the occurrence of Stage 4-5
more » ... seizures, and the seizure activity of kindling was recorded for 30 min. In an acute PTZ challenge study, 60 mg/kg PTZ was used to induce kindled seizure. Injection of carnosine (200, 500 mg/kg, i.p.) significantly decreased seizure stage, and prolonged the latencies for myoclonic jerks, in a dose-and time-dependent manner. In the seizure development process, 500 mg/kg carnosine also significantly delayed the onset of PTZ kindled seizures. In addition, carnosine significantly reversed decreased histamine levels induced by PTZ kindled seizure in the hippocampus. These results indicate that carnosine can protect against PTZ-induced seizures in both the development of kindling and the challenge process in rats. The results suggest that carnosine might be an endogenous anticonvulsant factor in the brain and can be used as a new antiepileptic drug in future.
doi:10.1016/j.neulet.2006.02.031 pmid:16515835 fatcat:7e3t2u4dyne7zno2ftmbbwkxre

Plasma levels and expression of interleukin‑37 in patients with immune thrombocytopenia

Feng Zhang, Xiao‑Juan Zhu, Xiao‑Jing Zhu, Yan‑Xia Liu, Ting Yuan, Qing‑Min Yao
2019 Experimental and Therapeutic Medicine  
Interleukin (IL)-37 has an important role in autoimmune diseases by suppressing immunity and inflammation; however, the role of IL-37 in immune thrombocytopenia (ITP) has remained largely elusive. The present study aimed to investigate the expression of IL-37 and its potential role in the pathogenesis of ITP. The plasma levels and expression of IL-37 in the peripheral blood mononuclear cells of patients with active ITP, ITP patients in remission and healthy controls were measured by ELISA and
more » ... verse transcription-quantitative PCR, respectively. The levels of IL-37 in patients with ITP treated with and without glucocorticoids were also determined by ELISA. Specific anti-platelet glycoprotein (GP)IIb/IIIa and/or GPIb/IX autoantibodies were assayed by modified monoclonal antibody-specific immobilization of platelet antigens. The mean value of plasma IL-37 in ITP patients was slightly higher than that in healthy controls, but this was not statistically significant. There was no correlation between IL-37 and anti-platelet autoantibodies, and no significant difference in the IL-37 concentration was identified between patients treated with and without glucocorticoids. In addition, the correlation between IL-37 and the platelet count was analyzed, with no statistical significance observed. It was therefore concluded that IL-37 may not have a pivotal role in the development of ITP. However, the lack of significant differences may be due to the limited number of patients in different groups. A larger number of ITP patients should be enrolled in the future work and achieve more accurate results.
doi:10.3892/etm.2019.7824 pmid:31572521 pmcid:PMC6755492 fatcat:rmbizygifnam3hkhbvbigjoxbi

Exploration of pathological prediction of chronic kidney diseases by a novel theory of bi-directional probability

Yuan Yang, Min Luo, Li Xiao, Xue-jing Zhu, Chang Wang, Xiao Fu, Shu-guang Yuan, Fang Xiao, Hong Liu, Zheng Dong, Fu-you Liu, Lin Sun
2016 Scientific Reports  
In the clinic, the pathological types of chronic kidney diseases (CKD) are considered references for choosing treatment protocols. From a statistical viewpoint, a non-invasive method to predict pathological types of CKD is a focus of our work. In the current study, following a frequency analysis of the clinical indices of 588 CKD patients in the department of nephrology, a third-grade class-A hospital, a novel theory is proposed: "bi-directional cumulative probability dichotomy". Further, two
more » ... dels for the prediction and differential diagnosis of CKD pathological type are established. The former indicates an occurrence probability of the pathological types, and the latter indicates an occurrence of CKD pathological type according to logistic binary regression. To verify the models, data were collected from 135 patients, and the results showed that the highest accuracy rate on membranous nephropathy (MN-100%), followed by IgA nephropathy (IgAN-83.33%) and mild lesion type (MLN-73.53%), whereas lower prediction accuracy was observed for mesangial proliferative glomerulonephritis (0%) and focal segmental sclerosis type (21.74%). The models of bi-directional probability prediction and differential diagnosis indicate a good prediction value in MN, IgAN and MLN and may be considered alternative methods for the pathological discrimination of CKD patients who are unable to undergo renal biopsy. Chronic kidney disease (CKD) is a common chronic disease that occurs in the elderly population and presents with the characteristics of an estimated glomerular filtration rate of < 60 mL/min per 1.73 m 2 or a urinary albumin: creatinine ratio of > 30 mg/g 1 . Generally, CKD is classified as one of two main types in the clinical setting: chronic nephritis or nephrotic syndrome. Indications include oedema, high blood pressure, proteinuria, haematuria, hypoalbuminemia, hyperlipidaemia, and changes in urine. In addition, CKD can be present in a complex form, such as lupus nephritis, purpura nephritis, hypertensive nephropathy, diabetic nephropathy, obstructive nephropathy, vasculitis related kidney disease, and allergic interstitial nephritis due to the diversity of primary diseases or inducements. The discrimination of CKD pathological types is important for treatment in the clinic. The common types are described as a mild lesion type (MLN), focal segmental sclerosis type (FSGS), membranous nephropathy (MN), IgA nephropathy (IgAN), and mesangial proliferative glomerulonephritis (MsPGN) . However, the present method of identifying CKD pathological type is through a renal y biopsy, which necessitates certain surgical procedures and specific histochemical staining. Under some conditions, it is not feasible to perform a renal biopsy in CKD patients due to clinical condition or non-adherence. Statistics can be used to evaluate the regularity of CKD pathological types associated with clinical data in CKD patients, such as gender, age, occupation, disease duration, inducements, first medication, clinical signs, and the laboratory results of blood or urine assays. Currently, the statistical study of predicting pathological types of CKD patients is still a new field, which is helpful for clinical diagnosis and treatment. In the current study, to determine whether the CKD pathological type is associated with the frequency of clinical signs of CKD patients, the clinical data of CKD patients, including their basic characteristics, medical history, results of clinical and laboratory exams, were analyzed. A
doi:10.1038/srep32151 pmid:27557856 pmcid:PMC4997332 fatcat:uwmaxmrzezfbxl6pjd4nxoloky

Interaction Between Photoreceptors and BR Signaling in Arabidopsis

Zhong Xin Zhu, Xiao Feng Zhu, Yu Ting Zhu, Da Nian Yao, Yuan Hu Xuan
2015 Acta Biologica Cracoviensia Series Botanica  
Extensive studies have been performed to elucidate the role of brassinosteroids (BRs), an important class of phy-tohormone in plant growth, development, and photomorphogenesis. Different wavelengths of light recognized by photoreceptors play a crucial role in plant development. The role of different photoreceptors in BR signaling has not been analyzed. Here we used photoreceptor single mutants, double mutants and even a quadruple mutant to analyze BR-dependent hypocotyl growth and gene
more » ... n. All the photoreceptor mutants differed from the controls in their response to BR, and hypocotyl elongation as well as BR marker gene regulation were inhibited by application of propiconazole (PCZ), a BR biosynthesis inhibitor. In addition, altered Phytochrome and Cryptochrome expression in brassinosteroid insensitive 1 mutant bri1-5 and brassinazole-resistant 1 dominant mutant bzr1-D indicated that BR negatively regulates photoreceptors in transcriptional levels. This is the first study to investigate the connections between BR and photoreceptors in Arabidopsis.
doi:10.2478/abcsb-2014-0027 fatcat:reniys44gzh3pihw36mupq3fiu
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