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Yu Alexander Tse, Shuai Liu, Yang Yang, Member, IEEE, and Michael Yu Wang, Fellow, IEEE TABLE I . I LINEAR CONNECTION FORCE TEST RESULTSFig. 7. ... Tse, S. Liu and Y. Yang are with the Department of Mechanical and Aerospace Engineering, Hong Kong University of Science and Technology, Hong Kong (e-mail: email@example.com; firstname.lastname@example.org). Y. ...arXiv:2004.04976v1 fatcat:5izjzduenfbx3crba7erl2ssfy
Tse (corresponding author) are with the Department of Mechanical and Aerospace Engineering, Hong Kong University of Science and Technology, Hong Kong (e-mail: email@example.com; firstname.lastname@example.org ...arXiv:2105.00680v1 fatcat:yhn6rbntsvhlvahw4kgf6kwtnq
Sjögren syndrome (SS) or dry eye disease (DED) is one of the most complicated ocular surface diseases. The goal of this study is to elucidate the relationship of the changes in clinical indices of tear film (TF) homeostasis with respect to tear components to allow for SS-DED monitoring and avoid stably controlled SS-DED patients from re-entering a vicious cycle. This prospective case-control study compared stable SS-DED patients with non-SS-DED control from several aspects, including clinicaldoi:10.3390/ijms20081932 fatcat:oy4voluiizgmletkerjggyt6ze
more »... dices for TF homeostasis, 2 DED diagnostic biomarkers (MMP-9 and lactoferrin), and the proteome of flush tears. Compared with non-SS-DED controls, stably controlled SS-DED subjects had less tear secretion and higher ocular surface inflammation, a higher concentration ratio of tear MMP-9/lactoferrin, a more diverse tear proteome, and lower spectral intensities of lipocalin-1, lacritin, and prolactin-inducible protein among the abundant tear proteins. For stable SS-DED patients, the concentration ratio of tear MMP-9/lactoferrin and the corrected lipocalin-1 signal was positively correlated with ocular inflammation and TF stability, respectively. MMP-9 released from stressed ocular surface epithelium and lipocalin-1 secreted from the energetic lacrimal gland are two tear biomarkers responding well to TF homeostasis. The tear proteomics approach through flush tears is a promising method for monitoring SS-DED patients with a standardized sampling procedure and lactoferrin-corrected analysis.
Acknowledgements The author would like to thank doctor Jen-chia Tsai, Yi-hao Chen, I-hui Yang, Hun-Ju Yu, Ho Jen-Wen, and Wei-Yu Chiang for their efforts on making the clinical diagnosis for these validation ...doi:10.1038/s41598-020-71425-9 pmid:32879364 fatcat:ynngs2ktb5f7tjpbcqq3tpk57m
Journal of Fungi
Acknowledgments: We thank Yu-Ting Huang for her assistance with connecting investigators from different medical centers and laboratories. ...doi:10.3390/jof8010064 pmid:35050004 pmcid:PMC8777873 fatcat:u7b2ztrwq5glnprfidsuebdpla
A sound ocular surface microbiota has been recognized as a part of ocular surface health following a growing body of evidence from next-generation sequencing technique and metagenomic analysis. However, even from the perspective of contemporary precision medicine, it is difficult to directly apply these new technologies to clinical practice. Therefore, we proposed a model based on dot hybridization assay (DHA) to bridge conventional culture with a metagenomic approach in investigating anddoi:10.3390/ijms21218299 pmid:33167500 fatcat:jap2h4rkenaq5aaqpghwlncsxu
more »... ring ocular surface microbiota. Endophthalmitis, mostly caused by bacterial infection, is the most severe complication of many intraocular surgeries, such as cataract surgery. Hazardous microorganisms hiding and proliferating in the ocular surface microbiota not only increase the risk of endophthalmitis but also jeopardize the effectiveness of the preoperative aseptic procedure and postoperative topical antibiotics. The DHA model enables the simultaneous assessment of bacterial bioburden, detection of target pathogens and microorganisms, and surveillance of methicillin/oxacillin resistance gene mecA in the ocular surface microbiota. This assay revealed heavier bacterial bioburden in men, compatible with a higher risk of endophthalmitis in male patients who underwent cataract surgery. No occurrence of endophthalmitis for these patients was compatible with non-hazardous microorganisms identified by specific dots for target pathogens. Moreover, the mecA dot detected oxacillin-resistant strains, of which culture failed to isolate. Therefore, the DHA model could provide an alternative genomic approach to investigate and monitor ocular surface microorganisms in clinical practice nowadays.
., 1996) , DNA-damaging drugs (Yu et al., 1996a) , pro-in¯ammatory cytokines (Raingeaud et al., 1995) , or chemopreventive agents (Yu et al., 1996b) . ... The sequence of the primers were presented elsewhere (Yu et al., 1996a) . ...doi:10.1038/sj.onc.1202305 pmid:9927194 fatcat:74zcvanajbbdtpquaxa2jdvnay
Most studies about dry eye disease (DED) chose unilateral eye for investigation and drew conclusions based on monocular results, whereas most studies involving tear proteomics were based on the results of pooling tears from a group of DED patients. Patients with DED were consecutively enrolled for binocular clinical tests, tear biochemical markers of DED, and tear proteome. We found that bilateral eyes of DED patients may have similar but different ocular surface performance and tear proteome.doi:10.3390/ijms22010422 pmid:33401599 fatcat:pfdbx7ttt5dmpbmjyi3dybnwym
more »... ost ocular surface homeostatic markers and tear biomarkers were not significantly different in the bilateral eyes of DED subjects, and most clinical parameters and tear biomarkers were correlated significantly between bilateral eyes. However, discrepant binocular presentation in the markers of ocular surface homeostasis and the associations with tear proteins suggested that one eye's performance cannot represent that of the other eye or both eyes. Therefore, in studies for elucidating tear film homeostasis of DED, we may lose some important messages hidden in the fellow eye if we collected clinical and proteomic data only from a unilateral eye. For mechanistic studies, it is recommended that researchers collect tear samples from the eye with more severe DED under sensitive criteria for identifying the more severe eye and evaluating the tear biochemical and proteomic markers with binocular concordance drawn in prior binocular studies.
The Yu laboratory is funded by the Medical Research Council, Cancer Research UK, the Generation Trust, the Academy of Medical Sciences, the Wellcome Trust, and the British Heart Foundation. ... ., 2010; Yu et al., 2005) and mammalian cells (Martinsson-Ahlzé n et al., Westbrook et al., 2007) . ...doi:10.1016/j.devcel.2012.06.018 pmid:22898779 pmcid:PMC3898080 fatcat:3rdqbq6zzfcxje763alj6yhaee
These authors contributed equally to this work. Abstract In mitotic cells, the cyclin-dependent kinase (CDK) subunit protein CKS1 regulates S phase entry by mediating degradation of the CDK inhibitor p27. Although mature neurons lack mitotic CDKs, we found that CKS1 was actively expressed in postmitotic neurons of the adult hippocampus. Interestingly, Cks1 knockout (Cks1 −/− ) mice exhibited poor long-term memory, and diminished maintenance of long-term potentiation in the hippocampal circuits.doi:10.1093/cercor/bhw354 pmid:28365778 pmcid:PMC5939225 fatcat:mqcrxswghrbflmx76bru5vo2ia
more »... Furthermore, there was neuronal accumulation of cofilin-actin rods or cofilin aggregates, which are associated with defective dendritic spine maturation and synaptic loss. We further demonstrated that it was the increased p27 level that activated cofilin by suppressing the RhoA kinase-mediated inhibitory phosphorylation of cofilin, resulting in the formation of cofilin aggregates in the Cks1 −/− which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. neuronal cells. Consistent with reports that the peptidyl-prolyl-isomerase PIN1 competes with CKS1 for p27 binding, we found that inhibition of PIN1 diminished the formation of cofilin aggregates through decreasing p27 levels, thereby activating RhoA and increasing cofilin phosphorylation. Our results revealed that CKS1 is involved in normal glutamatergic synapse development and dendritic spine maturation in adult hippocampus through modulating p27 stability.
Hutchinson-Gillford Progeria Syndrome (HGPS) is a fatal genetic disorder characterized by premature aging in multiple organs including the skin, musculoskeletal and cardiovascular systems. It is believed that an increased mechanosensitivity of HGPS cells is a causative factor for vascular cell death and vascular diseases in HGPS patients. However, the exact mechanism is unknown. Transient receptor potential (TRP) channels are cationic channels that can act as cellular sensors for mechanicaldoi:10.1371/journal.pone.0087273 pmid:24475260 pmcid:PMC3903625 fatcat:hck2jg3avrbe7igewig3a4mxbe
more »... uli. The aim of this present study was to examine the expression and functional role of TRP channels in human induced pluripotent stem cell-derived endothelial cells (iPSC-ECs) from the patients with HGPS. The mRNA and protein expression of TRP channels in HGPS and control (IMR90) iPSC-ECs were examined by semi-quantitative RT-PCRs and immunoblots, respectively. Hypotonicity-induced cytosolic Ca 2+ ([Ca 2+ ] i ) rise in iPSC-ECs was measured by confocal microscopy. RT-PCRs and immunoblots showed higher expressional levels of TRPV2 in iPSC-ECs from HGPS patients than those from normal individuals. In functional studies, hypotonicity induced a transient [Ca 2+ ] i rise in iPSC-ECs from normal individuals but a sustained [Ca 2+ ] i elevation in iPSC-ECs from HGPS patients. A nonselective TRPV inhibitor, ruthenium red (RuR, 20 mM), and a specific TRPV2 channel inhibitor, tranilast (100 mM), abolished the sustained phase of hypotonicityinduced [Ca 2+ ] i rise in iPSC-ECs from HGPS patients, and also markedly attenuated the transient phase of the [Ca 2+ ] i rise in these cells. Importantly, a short 10 min hypotonicity treatment caused a substantial increase in caspase 8 activity in iPSC-ECs from HGPS patients but not in cells from normal individuals. Tranilast could also inhibit the hypotonicity-induced increase in caspase 8 activity. Taken together, our data suggest that an up-regulation in TRPV2 expression causes a sustained [Ca 2+ ] i elevation in HGPS-iPSC-ECs under hypotonicity, consequently resulting in apoptotic cell death. This mechanism may contribute to the pathogenesis of vascular diseases in HGPS patients.
Huang and Kuan-Yu Chen designed research; Alexander T. H. ... Science and Technology of Taiwan (MOST107-2320-B-182A-007-) and Chang-Gung Memorial Hospital Research Foundation (CMRPG2G0331 and CMRPG2G0332) to Tse-Hung Huang, by grant from 102CM-TMU-03 to Alexander ...doi:10.1111/jcmm.14689 pmid:31638335 pmcid:PMC6850923 fatcat:2kfs5ikkqnbx3d2cmmnlfe2i6q
Systemic lupus erythematosus (SLE) has a complex etiology and is affected by both genetic and environmental factors. Although more than 40 loci have shown robust association with SLE, the details of these loci, such as the independent contributors and the genes involved, are still unclear. In this study, we performed meta-analysis of two existing genome-wide association studies (GWASs) on Chinese Han populations from Hong Kong and Anhui, China, and followed the findings by further replicationdoi:10.1093/hmg/ddt424 pmid:24001599 fatcat:pwfdaee7rjgtleqaxpvqa5lprq
more »... three additional Chinese and Thailand cohorts with a total of 4254 cases and 6262 controls matched geographically and ethnically. We discovered multiple susceptibility variants for SLE in the 11q23.3 region, including variants in/near PHLDB1 (rs11603023, P_ combined 5 1.25E208, OR 5 1.20), DDX6 (rs638893, P_ combined 5 5.19E207, OR 5 1.22) and CXCR5 (rs10892301, P_ combined 5 2.51E208, OR 5 0.85). Genetic contributions from the newly identified variants were all independent of SNP rs4639966, whose association was reported from the previous GWAS. In addition, the three newly identified variants all showed independent association with the disease through modeling by * both stepwise and conditional logistic regression. The presence of multiple independent variants in this region emphasizes its role in SLE susceptibility, and also hints the possibility that distinct biological mechanisms might be involved in the disease involving this genomic region.
Jesús Cobo Tokgöz, Emre Tomaszuk, Dominik Töreyin, Hakan Torregrosa Sanchez, Juan Ramon Torres-Moreno, Juan-Manuel Trainor, Patrick Trujillo, Leonardo Tsagkatakis, Grigorios Tsai, Pei-Wei Tsai, Du-Ming Tse ... Wu, Nan Wu, Jiunn-Lin Xie, Lu Xu, Xun Yamakami, Akebo Yamamoto, Toshiyuki Yang, Yongjie Yang, Tiantian Yeh, Sheng Lih Yeum, Chul Min Yu, Chenglong Yu, Xiao-Hua Yun, Beong In ...doi:10.3390/a11010011 fatcat:c24jr5pnjrcxhdjnnm4fynkwea
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