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Introduction: The effects of Jin-Ying-Tang (JYT) on Toll-like Receptor 4 (TLR4) signalling transduction of lipopolysaccharide (LPS)-stimulated mouse mammary epithelial cells (MECs) in vitro were examined ... Jin-Ying-Tang (JYT) is an empirical formula that was developed based on the principles of traditional Chinese medicine (TCM) and is used to treat mastitis in dairy cattle. ... Bunge., root Angelica sinensis(Oliv.)Diels., root Herbs Chinese name Origin (China) Batch number Dosage (g) Shan yin hua Pu gong ying Gua lou Lian qiao Da huang Huang qi Dang gui Guizhou ...doi:10.1515/jvetres-2016-0011 fatcat:l7gatvo2zfhuvhhz6hcmfj2d4a
Accumulating evidence indicates that L-carnitine (LC) protects against multiorgan damage through its antioxidant properties and preservation of the mitochondria. Little information is available about the effects of LC on renal fibrosis. This study examined whether LC treatment would provide renoprotection in a rat model of unilateral ureteral obstruction (UUO) and in vitro. Sprague-Dawley rats that underwent UUO were treated daily with LC for 7 or 14 days. The influence of LC on renal injurydoi:10.3904/kjim.2019.413 pmid:32942841 pmcid:PMC8009152 fatcat:g54len5cwjcthcxwffjvhurefq
more »... sed by UUO was evaluated by histopathology, and analysis of gene expression, oxidative stress, mitochondrial function, programmed cell death, and phosphatidylinositol 3-kinase (PI3K)/ AKT/forkhead box protein O 1a (FoxO1a) signaling. In addition, H2O2-exposed human kidney cells (HK-2) were treated with LC. LC treatment inhibited expression of proinflammatory and profibrotic cytokines, and was followed by a significant attenuation of tubulointerstitial inflammation and fibrosis. The increased oxidative stress caused by UUO was associated with mitochondrial dysfunction and excessive apoptosis and autophagy via PI3K/AKT/FoxO1a-dependent signaling, and this was abrogated by administration of LC. In H2O2-exposed HK-2 cells, LC decreased intracellular production of reactive oxygen species, and suppressed expression of profibrotic cytokines and reduced the number of apoptotic cells. LC protects against the progression of tubulointerstitial fibrosis in an obstructed kidney.
Uncovering the risk transmitting path within economic sectors in China is crucial for understanding the stability of the Chinese economic system, especially under the current situation of the China-US trade conflicts. In this paper, we try to uncover the risk spreading channels by means of volatility spillovers within the Chinese sectors using stock market data. By applying the generalized variance decomposition framework based on the VAR model and the rolling window approach, a set ofarXiv:2002.09097v1 fatcat:bpiapzjtbfc73gn3lxf3p5eth4
more »... ness matrices is obtained to reveal the overall and dynamic spillovers within sectors. It is found that 17 sectors (mechanical equipment, electrical equipment, utilities, and so on) are risk transmitters and 11 sectors (national defence, bank, non-bank finance, and so on) are risk takers during the whole period. During the periods with the extreme risk events (the global financial crisis, the Chinese interbank liquidity crisis, the Chinese stock market plunge, and the China-US trade war), we observe that the connectedness measures significantly increase and the financial sectors play a buffer role in stabilizing the economic system. The robust tests suggest that our results are not sensitive to the changes of model parameters. Our results not only uncover the spillover effects within the Chinese sectors, but also highlight the deep understanding of the risk contagion patterns in the Chinese stock markets.
Lupeol exhibits novel physiological and pharmacological activities, such as anticancer and immunity-enhancing activities. However, cytotoxicity remains a challenge for triterpenoid overproduction in microbial cell factories. As lipophilic and relatively small molecular compounds, triterpenes are generally secreted into the extracellular space. The effect of increasing triterpene efflux on the synthesis capacity remains unknown. In this study, we developed a strategy to enhance triterpene effluxdoi:10.1186/s13068-020-01773-1 pmid:32760447 pmcid:PMC7392732 fatcat:l65hk5aibvdfrpen4tyal2kdpe
more »... through manipulation of lipid components in Y. lipolytica by overexpressing the enzyme Δ9-fatty acid desaturase (OLE1) and disturbing phosphatidic acid phosphatase (PAH1) and diacylglycerol kinase (DGK1). By this strategy combined with two-phase fermentation, the highest lupeol production reported to date was achieved, where the titer in the organic phase reached 381.67 mg/L and the total production was 411.72 mg/L in shake flasks, exhibiting a 33.20-fold improvement over the initial strain. Lipid manipulation led to a twofold increase in the unsaturated fatty acid (UFA) content, up to 61-73%, and an exceptionally elongated cell morphology, which might have been caused by enhanced membrane phospholipid biosynthesis flux. Both phenotypes accelerated the export of toxic products to the extracellular space and ultimately stimulated the capacity for triterpenoid synthesis, which was proven by the 5.11-fold higher ratio of extra/intracellular lupeol concentrations, 2.79-fold higher biomass accumulation and 2.56-fold higher lupeol productivity per unit OD in the modified strains. This strategy was also highly efficient for the biosynthesis of other triterpenes and sesquiterpenes, including α-amyrin, β-amyrin, longifolene, longipinene and longicyclene. In conclusion, we successfully created a high-yield lupeol-producing strain via lipid manipulation. We demonstrated that the enhancement of lupeol efflux and synthesis capacity was induced by the increased UFA content and elongated cell morphology. Our study provides a novel strategy to promote the biosynthesis of valuable but toxic products in microbial cell factories.
In this study, we used RNA sequencing (RNA-seq) to analyze and compare bulk cell samples from wild-type (WT) dermal mesenchymal stem cells (DMSCs) (n = 3) and Prx II knockout DMSCs (n = 3). The purpose of the study was to elucidate the role of Prx II on allogeneic immune rejection of transplanted DMSCs. The results revealed differential expression of 472 genes (176 up-regulated and 296 down-regulated; p ≤ 0.05) between the PrxII +/+ (WT) and PrxII −/− sample groups. When highly regulated genesdoi:10.1186/s13765-020-00515-z fatcat:gtqcfrcixfa7nmmo2lkwqparfy
more »... ere categorized according to the Gene Ontology (GO) molecular function classification and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, the PrxII −/− samples showed a robust downward trend in allograft rejection. The study identified 43 all immunologically rejected differentially expressed genes, of which 41 showed lower expression in the PrxII −/− vs. PrxII +/+ (WT) samples. These findings suggest that Prx II gene knockout may down-regulate the allograft rejection that occurs during DMSCs transplantation and improve the survival rate of DMSCs in the host. This study provides a new perspective on the clinical treatment of stem cell transplantation.
Fluorescence is a powerful tool for in-vivo imaging in living animals. The traditional in-vivo fluorescence imaging equipment is based on single-view two-dimensional imaging systems. However, they cannot meet the needs for accurate positioning during modern scientific research. A near-infrared in-vivo fluorescence imaging system is demonstrated, which has the capability of deep source signal detecting and three-dimensional positioning. A three-dimensional coordinates computing (TDCP) methoddoi:10.1117/1.jbo.19.11.116002 pmid:25364949 fatcat:j4krtrn7jbgipfxuvh7ufa5ddm
more »... uding a preprocess algorithm is presented based on binocular stereo vision theory, to figure out the solution for diffusive nature of light in tissue and the emission spectra overlap of fluorescent labels. This algorithm is validated to be efficient to extract targets from multispectral images and determine the spot center of biological interests. Further data analysis indicates that this TDCP method could be used in three-dimensional positioning of the fluorescent target in small animals. The study also suggests that the combination of a large power laser and deep cooling charge-coupled device will provide an attractive approach for fluorescent detection from deep sources. This work demonstrates the potential of binocular stereo vision theory for three-dimensional positioning for living animal in-vivo imaging. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
In keeping with this functional spectrum, the most significant TFBSs included those of multiple organogenesis-related regulators such as the nervous system-specific OCT1 (Jin et al., 2009) and BRN2 ...doi:10.1016/j.devcel.2010.06.014 pmid:20643359 fatcat:lencjz72zrfxxkta3ieou37sam
Jin, L.H. Xie, H.W. Yue  considered the locally upper bounded maps with values in the ordered topological vector spaces and provided new monotone insertion theorems. ...arXiv:2002.12742v1 fatcat:tak2von535fbvarkdmipl72tta
However, as a non-conventional yeast, the capability of selective and tunable perturbation Open Access Microbial Cell Factories *Correspondence: email@example.com † Jin-lai Zhang and Yang-Zi Peng ...doi:10.1186/s12934-018-0909-8 pmid:29678175 pmcid:PMC5910576 fatcat:x4tkn2ckjzc2vhgyykhiqpaqje
An important unresolved clinical issue is to distinguish hepatitis B virus (HBV) infection caused chronic hepatitis and their corresponding liver cirrhosis (LC). Recent research suggests that circulating microRNAs are useful biomarkers for a wide array of diseases. We analyzed microRNA profiles in the plasmas of a total of 495 chronic hepatitis B (CHB) patients, LC patients and healthy donors and identified 10 miRNAs that were differentially expressed between CHB and LC patients. Our logisticdoi:10.1038/srep15026 pmid:26456479 pmcid:PMC4601029 fatcat:n4utdc5duje4hljgx2x7alqdby
more »... dels show that three panels of miRNAs have promising diagnostic performances in discriminating CHB from LC. Blinded tests were subsequently conducted to evaluate the diagnostic performances in clinical practice and a sensitivity of 85% and specificity of 70% have been achieved in separating CHB from LC pateints. The expression levels of some circulating miRNAs were significantly correlated with HBV DNA load and liver function, such as prothrombin activity (PTA) and levels of alanin aminotransferase (ALT), albumin (ALB) and cholinesterase (CHE). Our results provide important information for developing novel diagnostic tools for distinguishing chronic HBV hepatitis and their corresponding cirrhosis. Chronic HBV infection is an important cause of viral hepatitis (CHB) and liver cirrhosis (LC) worldwide. It is estimated that there are more than 350 million people with chronic HBV infection and more than one million patients die each year 1 . Despite effective vaccination that has resulted in a decrease of acute HBV incidences in many countries, persistent infection of HBV remains a principle challenge. Therefore, it is very important to have accurate method to diagnose and evaluate CHB and LC related to chronic HBV infection (LC for short in the following part) safely and conveniently. HBV infection and its disease progression are often monitored by clinical parameters. Biopsy is an invasive method with good diagnostic performance, but for its risk of various complications, it is difficult to be performed routinely. Noninvasive methods include blood tests and image examination, including aminotransferases (ALT, AST), fibrosis related factors, HBV DNA levels and the presence of HBV antigens 2-4 , but these clinical parameters have limited use in distinguishing patients who have chronic hepatitis from those who have developed cirrhosis 5 . None of the methods could discriminate CHB patients from chronic HBV carriers with no clinical symptoms or discriminate LC patients at the early stage from CHB patients. Based on the reasons above, it is urgently needed to develop noninvasive predictive biomarkers and methods to improve the diagnostic accuracy for CHB and LC.
Chemistry is the molecular basis of life activities. Accurate acquisition of the distribution and composition variation of physiologically important molecules in vivo is of great significance for understanding sophisticated physiological and pathological processes of the human body. Electrochemical approaches, especially electrochemical aptamer-based biosensors, have the excellent characteristics of high specificity, a wide range of target molecules, and easy miniaturization, providing andoi:10.1360/ssc-2022-0008 fatcat:fkrfobczofbntmjh3ifvygeszq
more »... ive tool for rapid, sensitive, and highly selective detection in complex physiological environments. In this review, we briefly summarize the design of the nucleic-acid-aptamer-based electrochemical biosensor and its application in in vivo analysis, and its future prospects and challenges were also discussed.
Gui-Nan Shen, Yong-Zhe Jin, Yu Jin, Ying-Hao Han, Li Feng, Lei Liu, Mei-Hua Jin, Ying-Hua Luo, Tea-Ho Kwon, and Yu-Dong Cui: Performed the experiments; Analyzed and interpreted the data; Contributed reagents ... Cheng-Hao Jin: Conceived and designed the experiments. ...doi:10.1016/j.heliyon.2016.e00132 pmid:27512726 pmcid:PMC4971128 fatcat:fmvcsblyjvgfpe6a6ywjocid5m
Bladder cancer is one of the most malignant tumors closely associated with macrophage immune dysfunction. The Chinese medicine polyporus has shown excellent efficacy in treating bladder cancer, with minimal side effects. However, its material basis and mechanism of action remain unclear. A new water-soluble polysaccharide (HPP) with strong immunomodulatory activity was isolated from the fungus Polyporus umbellatus (Pers.) Fries. HPP had an average molecular weight of 6.88 kDa and was composeddoi:10.3389/fchem.2020.00581 pmid:32850623 pmcid:PMC7399574 fatcat:73wqjvyrqbgljj5h2dexcejjz4
more »... inly of an <-(1 → 4)-linked D-galactan backbone. The immunomodulatory activity of HPP was determined in vitro, and the results revealed that it could obviously increase the secretion of immune factors by IFN-γ-stimulated macrophages, including nitric oxide (NO), interleukin-6 (IL-6), interleukin-1β (IL-1β), RANTES and interleukin-23 (IL-23), and the expression of the cell membrane molecule CD80. In addition, HPP was recognized by Toll-like receptor 2 (TLR2) and activated the signaling pathways of NF-κB and NLRP3 in a bladder cancer microenvironment model, indicating that HPP could enhance host immune system function. These findings demonstrated that HPP may be a potential immune modulator in the treatment of immunological diseases or bladder cancer therapy.
Human blastocysts are comprised of the first three cell lineages of the embryo: trophectoderm, epiblast, and primitive endoderm, all of which are essential for early development and organ formation1,2. However, due to ethical concerns and restricted access to human blastocysts, we lack a comprehensive understanding of early human embryogenesis. To bridge this knowledge gap, we need a reliable model system that recapitulates early stages of human embryogenesis. Here we report adoi:10.1101/2021.03.09.434313 fatcat:k5vexieeive4xglgrshcvfzufu
more »... (3D), two-step induction protocol for generating blastocyst-like structures (EPS-blastoids) from human extended pluripotent stem (EPS) cells. Morphological and single-cell transcriptomic analyses revealed that EPS-blastoids contain key cell lineages and are transcriptionally similar to human blastocysts. Furthermore, EPS-blastoids also exhibited the developmental potential to undergo post-implantation morphogenesis in vitro to form structures with a cellular composition and transcriptome signature similar to human embryos that had been cultured in vitro for 8 or 10 days. In conclusion, human EPS-blastoids provide a new experimental platform for studying early developmental stages of the human embryo.
The authors thank the National Natural Science Foundation of China (No. 20471014), the Fok Ying Tung Education Foundation, the Natural Science Foundation of Jilin Province (China) and the Science Foundation ...doi:10.1107/s1600536805033714 fatcat:m7zxp32pivgmpipsymraowi7vi
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