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Habituation, latent inhibition, and extinction

Wesley P. Jordan, Travis P. Todd, David J. Bucci, Robert N. Leaton
2015 Learning & Behavior  
., Jordan, Strasser, & McHale, 2000; Leaton & Borszcz, 1990) . Alternatively, the UR to the CS in the NPE group could be reduced by limited stimulus presentations during preexposure.  ... 
doi:10.3758/s13420-015-0168-z pmid:25678396 fatcat:pkfrwizndvdl3glzgvkh5xvj2u

What Works in Community-Led Suicide Prevention: Perspectives of Wesley LifeForce Network Coordinators

Lennart Reifels, Amy Morgan, Lay San Too, Marisa Schlichthorst, Michelle Williamson, Helen Jordan
2021 International Journal of Environmental Research and Public Health  
The Wesley Lifeforce Suicide Prevention Networks program consists of a national cohort of local community-led suicide prevention networks.  ...  Acknowledgments: The research team wishes to express gratitude to all members of Wesley LifeForce networks, the Wesley LifeForce program team, and the Expert Advisory Group, who generously gave their time  ...  Wesley LifeForce Suicide Prevention Networks Wesley Mission, through its Wesley LifeForce Suicide Prevention Networks program (Wesley LifeForce program), has been working with Australian communities to  ... 
doi:10.3390/ijerph18116084 pmid:34200035 pmcid:PMC8200208 fatcat:bgyc2scdszfrljouot4yv2qtw4

Cardiovascular effects of phentermine and topiramate

Jens Jordan, Arne Astrup, Stefan Engeli, Krzysztof Narkiewicz, Wesley W. Day, Nick Finer
2014 Journal of Hypertension  
The paper by Jordan et al. provides an updated review on the cardiovascular effects of this new therapeutic approach which, along with its significant and sustained weight loss effects, improves triglycerides  ... 
doi:10.1097/hjh.0000000000000145 pmid:24621808 pmcid:PMC4011567 fatcat:oqaherezfzgd5l2jbls3twsmtq

Mobile Mink

Wesley Jordan, Mat Kelly, Justin F. Brunelle, Laura Vobrak, Michele C. Weigle, Michael L. Nelson
2015 Proceedings of the 15th ACM/IEEE-CE on Joint Conference on Digital Libraries - JCDL '15  
This work was performed as part of Wesley Jordan's mentorship at The MITRE Corporation.  ... 
doi:10.1145/2756406.2756956 dblp:conf/jcdl/JordanKBVWN15 fatcat:nejjsw5ftva4dhte4go2nyywym

A general Approach to Measure Three-Dimensional Forces from Cells

Wesley R. Legant, Jordan S. Miller, Brandon L. Blakely, Daniel M. Cohen, Guy M. Genin, Christopher S. Chen
2011 Biophysical Journal  
A general Approach to Measure Three-Dimensional Forces from Cells Wesley R. Legant 1 , Jordan S. Miller 1 , Brandon L. Blakely 1 , Daniel M. Cohen 1 , Guy M. Genin 2 , Christopher S.  ... 
doi:10.1016/j.bpj.2010.12.1258 fatcat:65pmnp2flve7zodtzqmkztqodu

Bioactive hydrogels made from step-growth derived PEG–peptide macromers

Jordan S. Miller, Colette J. Shen, Wesley R. Legant, Jan D. Baranski, Brandon L. Blakely, Christopher S. Chen
2010 Biomaterials  
Synthetic hydrogels based on poly(ethylene glycol) (PEG) have been used as biomaterials for cell biology and tissue engineering investigations. Bioactive PEG-based gels have largely relied on heterobifunctional or multi-arm PEG precursors that can be difficult to synthesize and characterize or expensive to obtain. Here, we report an alternative strategy, which instead uses inexpensive and readily available PEG precursors to simplify reactant sourcing. This new approach provides a robust system
more » ... n which to probe cellular interactions with the microenvironment. We used the step-growth polymerization of PEG diacrylate (PEGDA, 3400 Da) with bis-cysteine matrix metalloproteinase (MMP)-sensitive peptides via Michael-type addition to form biodegradable photoactive macromers of the form acrylate-PEG-(peptide-PEG) m -acrylate. The molecular weight (MW) of these macromers is controlled by the stoichiometry of the reaction, with a high proportion of resultant macromer species greater than 500 kDa. In addition, the polydispersity of these materials was nearly identical for three different MMP-sensitive peptide sequences subjected to the same reaction conditions. When photopolymerized into hydrogels, these high MW materials exhibit increased swelling and sensitivity to collagenase-mediated degradation as compared to previously published PEG hydrogel systems. Cell-adhesive acrylate-PEG-CGRGDS was synthesized similarly and its immobilization and stability in solid hydrogels was characterized with a modified Lowry assay. To illustrate the functional utility of this approach in a biological setting, we applied this system to develop materials that promote angiogenesis in an ex vivo aortic arch explant assay. We demonstrate the formation and invasion of new sprouts mediated by endothelial cells into the hydrogels from embedded embryonic chick aortic arches. Furthermore, we show that this capillary sprouting and three-dimensional migration of endothelial cells can be tuned by engineering the MMPsusceptibility of the hydrogels and the presence of functional immobilized adhesive ligands (CGRGDS vs. CGRGES peptide). The facile chemistry described and significant cellular responses observed suggest the usefulness of these materials in a variety of in vitro and ex vivo biologic investigations, and may aid in the design or refinement of material systems for a range of tissue engineering approaches.
doi:10.1016/j.biomaterials.2010.01.058 pmid:20138664 pmcid:PMC2837100 fatcat:q56bg3qbfbefxbdysaum5g5pny

Biorational management tactics to select against triazine-resistant Amaranthus hybridus: a field trial

Nicholas Jordan, Michael Kelrick, John. Brooks, Wesley Kinerk
1999 Journal of Applied Ecology  
KELRICK, JOHN BROOKS and WESLEY KINERK Science Division, Truman State University and Kirksville M063501, USA Summary 1.  ...  Jordan et al. Table I.  ... 
doi:10.1046/j.1365-2664.1999.00386.x fatcat:zna42u5mvncw7cuuqjh6qlssxq

Pain management of pancreatic head adenocarcinomas that are unresectable: celiac plexus neurolysis and splanchnicectomy

Wesley B Jones, Phillip Jordan, Maya Pudi
2015 Journal of Gastrointestinal Oncology  
Pancreatic adenocarcinoma is often incurable at the time of diagnosis. For patients with unresectable or recurrent disease, palliation of pain is a key component of care. Medical management with narcotics has numerous side effects and may be ineffective. Interventions for pain control include celiac plexus neurolysis (CPN) and splanchnicectomy. The purpose of this review is to outline pertinent anatomy, techniques, side effects, complications, and efficacy of interventions for palliation of
more » ... from pancreatic cancer. We reviewed current literature, as well as our own patients, to assess the role and outcomes of CPN and splanchnicectomy. Short descriptions of procedural techniques and functional illustrations are provided. Both CPN and splanchnicectomy have excellent outcomes with regard to pain control. Quality of life and survival, however, have not been conclusively demonstrated to improve with either technique. Data regarding head-to-head comparisons of the two interventions is lacking. Patients with incurable pancreatic carcinoma should be offered either CPN or splanchnicectomy when medical management with narcotics has failed.
doi:10.3978/j.issn.2078-6891.2015.052 pmid:26261731 pmcid:PMC4502156 fatcat:6rjdwrocazcdjdkg2hbgbrisei

GeneWeld: Efficient Targeted Integration Directed by Short Homology in Zebrafish

Jordan Welker, Wesley Wierson, Maira Almeida, Carla Mann, Melanie Torrie, Zhitao Ming, Stephen Ekker, Karl Clark, Drena Dobbs, Jeff Essner, Maura McGrail
2021 Bio-protocol  
Efficient precision genome engineering requires high frequency and specificity of integration at the genomic target site. Multiple design strategies for zebrafish gene targeting have previously been reported with widely varying frequencies for germline recovery of integration alleles. The GeneWeld protocol and pGTag (plasmids for Gene Tagging) vector series provide a set of resources to streamline precision gene targeting in zebrafish. Our approach uses short homology of 24-48 bp to drive
more » ... ed integration of DNA reporter cassettes by homology-mediated end joining (HMEJ) at a CRISPR/Cas induced DNA double-strand break. The pGTag vectors contain reporters flanked by a universal CRISPR sgRNA sequence to liberate the targeting cassette in vivo and expose homology arms for homology-driven integration. Germline transmission rates for precision-targeted integration alleles range 22-100%. Our system provides a streamlined, straightforward, and cost-effective approach for high-efficiency gene targeting applications in zebrafish. Graphic abstract: GeneWeld method for CRISPR/Cas9 targeted integration.
doi:10.21769/bioprotoc.4100 pmid:34395736 pmcid:PMC8329467 fatcat:7npsqxy4gfe6lebn7mzzvljosa

Response of Maize, Cotton, and Soybean to Increased Crop Density in Heterogeneous Planting Arrangements

Sandra R. Ethridge, Anna M. Locke, Wesley J. Everman, David L. Jordan, Ramon G. Leon
2022 Agronomy  
The reduction of row spacing and increase of crop population density are important tools for maximizing crop yield. For this strategy to be effective, the crop population should not create intraspecific crop competition that penalizes yield. Thus, planting arrangements that increase light interception throughout the canopy without increasing row spacing might be needed to maintain yield. In this study, heterogeneous planting arrangements on evenly spaced rows were analyzed for maize (Zea mays
more » ... ), cotton (Gossypium hirsutum L.), and soybean (Glycine max (L.) Merr.). Each crop had four planting arrangements: (1) normal density in all rows, considered the control, (2) doubled density in all rows, (3) a sequential arrangement of normal and tripled densities (each in every other row; NTNT), and (4) normal-tripled-tripled-normal (NTTN). Maize and cotton did not exhibit changes in growth and architecture when comparing uniform and variable planting arrangements. Soybeans were more adaptable and increased biomass production by 44% to 45% in variable arrangements. None of the crops showed differences in yield due to planting arrangement, so the use of rows with different densities might not be needed when using high densities to maximize yield.
doi:10.3390/agronomy12051238 fatcat:t5lvwjmgpfh77ia2cr3peq566e

Measurement of mechanical tractions exerted by cells in three-dimensional matrices

Wesley R Legant, Jordan S Miller, Brandon L Blakely, Daniel M Cohen, Guy M Genin, Christopher S Chen
2010 Nature Methods  
brief communications nature methods | ADVANCE ONLINE PUBLICATION | Methods using planar elastic surfaces or arrays of flexible cantilevers have been used to map, with subcellular resolution, the forces that cells generate against their substrates 1,5-7 . But many processes are altered when cells are removed from native three-dimensional (3D) environments and maintained on two-dimensional (2D) substrates. Cells encapsulated in a 3D matrix exhibit dramatically different morphology, cytoskeletal
more » ... ganization and focal adhesion structure from those on 2D substrates 8 . Even the initial means by which cells attach to and spread against a 2D substrate are quite different from the invasive process required for cells to extend inside a 3D matrix. These Quantitative measurements of cell-generated forces have heretofore required that cells be cultured on two-dimensional substrates. We describe a technique to quantitatively measure three-dimensional traction forces exerted by cells fully encapsulated in well-defined elastic hydrogel matrices. using this approach we measured traction forces for several cell types in various contexts and revealed patterns of force generation attributable to morphologically distinct regions of cells as they extend into the surrounding matrix. Cells are constantly probing, pushing and pulling on the surrounding extracellular matrix. These cell-generated forces drive cell migration and tissue morphogenesis, and maintain the intrinsic mechanical tone of tissues 1,2 . Such forces not only guide mechanical and structural events but also trigger signaling pathways that promote functions ranging from proliferation to stem-cell differentiation 3, 4 . Therefore, precise measurements of the spatial and temporal nature of these forces are essential to understanding when and where mechanical events come to play in both physiological and pathological settings.
doi:10.1038/nmeth.1531 pmid:21076420 pmcid:PMC3056435 fatcat:cgzyskbiyzbo7kfdmpxeg7acy4

Predicting division planes of three-dimensional cells by soap-film minimization [article]

Pablo Martinez, Lindy Allsman, Kenneth A Brakke, Christopher Hoyt, Jordan Hayes, Hong Liang, Wesley Neher, Yue Rui, Allyson Roberts, Amir Moradifam, Bob Goldstein, Charles Anderson (+1 others)
2017 bioRxiv   pre-print
One key aspect of cell division in multicellular organisms is the orientation of the division plane. Proper division plane establishment contributes to normal organization of the plant body. To determine the importance of cell geometry in division plane orientation, we designed a three-dimensional probabilistic mathematical modeling approach to directly test the century-old hypothesis that cell divisions mimic soap-film minima or that daughter cells have equal volume and the resulting division
more » ... lane is a local surface area minimum. Predicted division planes were compared to a plant microtubule array that marks the division site, the preprophase band (PPB). PPB location typically matched one of the predicted divisions. Predicted divisions offset from the PPB occurred when a neighboring cell wall or PPB was observed directly adjacent to the predicted division site, potentially to avoid creating a structurally unfavorable four-way junction. By comparing divisions of differently shaped plant and animal cells to divisions simulated in silico, we demonstrate the generality of this model to accurately predict in vivo division. This powerful model can be used to separate the contribution of geometry from mechanical stresses or developmental regulation in predicting division plane orientation.
doi:10.1101/199885 fatcat:d3mcad7ju5epzcqx4w3oq5c4p4

Guest Aggregation within Poly(L-lactic Acid)/Pluronic P104 Thin Films

Jordan M. Steves, Loraine T. Tan, Joseph A. Gardella, Robert Hard, Wesley L. Hicks, Alexander N. Cartwright, Bahattan Koc, Frank V. Bright
2008 Applied Spectroscopy  
Rhodamine 6G (R6G) doped thin films composed of poly(L-lactic acid) (PLLA) and Pluronic P104 were spin cast onto glass microscope slides and characterized by ultraviolet-visible, steady-state, and time-resolved fluorescence spectroscopy. The results show that R6G aggregation within the film increases as the R6G concentration and P104 loading increases. These results suggest an approach for studying drug distributions (monomers, aggregates) within biodegradable polymer formulations.
doi:10.1366/000370208783759605 pmid:18339236 fatcat:f5tv6445t5cdheje3zob4w6jm4

NOMADS: A Climate and Weather Model Archive at the National Oceanic and Atmospheric Administration

Glenn K. Rutledge, Jordan Alpert, Wesley Ebisuzaki
2006 Bulletin of The American Meteorological Society - (BAMS)  
Camp Springs, MD Never before has the U.S. maintained a digital archive for its operational weather models, and an innovative data access philosophy promotes interoperable access across the geosciences. Abstract An on-line archive of real-time and historical weather and climate model output and observational data is now available from the National Oceanic and Atmospheric
doi:10.1175/bams-87-3-327 fatcat:lsrhsxnj5bgltdwz5re3wxrgl4

The Effect of a High Fat Meal on Cerebral Vascular Function

Jordan C. Patik, Wesley J. Tucker, Bryon M. Curtis, Michael D. Nelson, R. Matthew Brothers
2017 Medicine & Science in Sports & Exercise  
It is well known that a single high fat meal (HFM) causes a robust and transient elevation in serum triglycerides (TG). This elevation in serum TG is a primary contributor to the post-prandial attenuation of peripheral vascular endothelial function, as assessed by flow-mediated dilation in the brachial artery. Whether a similar impairment in vascular reactivity can be observed in the cerebral circulation remains unknown, and was the focus of this investigation. PURPOSE: To test the hypothesis
more » ... at cerebral vascular function is impaired following a HFM. METHODS: End-tidal carbon dioxide partial pressure (PETCO2), middle cerebral artery blood velocity (MCAVmean), calculated cerebral vascular conductance index (CVCI; MCAVmean/mean arterial pressure) and cerebral vasodilator response to rebreathing induced hypercapnia (% increase in CVC from baseline at common maximal ΔPETCO2) were assessed in 6 healthy young men (27 ±5 years). Measures were assessed during fasted baseline and again at 2 and 4 h post meal consumption (HFM day) or at a similar time point in the fasted state (TC day). The two visits were separated by 2-7 days and were conducted in a randomized order. Blood lipids were assessed at baseline and at the 2 h time point into each respective condition. RESULTS: As expected, consumption of the HFM significantly elevated serum TG concentrations relative to TC at 2 h (HFM: 101±38 to 169±77mg/dl, TC: 107±32 to 92±31mg/dl, P=0.007). However, the HFM had no effect of cerebral vasodilator capacity during rebreathing induced hypercapnia. The maximal increase in %CVC achieved at the highest common ΔPETCO2 during all conditions within each subject was unchanged during 2hr and 4hr post HFM or TC (condition x time interaction: P=0.96). Similarly, the slope of the change in %CVC per change in ΔPETCO2 was unaffected by HFM across time (P=0.49). CONCLUSION: Contrary to our hypothesis, and unlike the peripheral vasculature, our preliminary data suggest that the cerebral circulation appears to be protected from the acute negative effects of a high fat meal.
doi:10.1249/01.mss.0000519188.38183.c8 fatcat:qzyxpz4n6zfs3d35hux5uzfm4i
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