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Improved antitumor efficacy of neutrophils stimulated by bacillus Calmette‑Gu�rin
2019
Molecular Medicine Reports
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Bacillus Calmette‑Guérin (BCG) has become a significant treatment for bladder cancer, and neutrophils are reported to be associated with the antitumor effect of BCG. The aim of the present study was to clarify the antitumor function of neutrophils stimulated by BCG. Initially, the killing effect and cytotoxic activity of neutrophils treated with BCG was detected. Subsequently, the effectiveness of BCG‑treated neutrophils extracted from tumor‑bearing mice was analyzed. The results revealed that
doi:10.3892/mmr.2019.10532
pmid:31524238
fatcat:btdpm3hoffbk7j3c7rmlk4ivje
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... he cytotoxic effect of neutrophils was stronger in the BCG‑treated group compared with that in the normal saline (NS)‑treated and control groups (P<0.05). A significantly higher concentration of cytokines tumor necrosis factor (TNF)‑α, interleukin (IL)‑1β, IL‑6 and TNF‑related apoptosis‑inducing ligand occurred in the BCG‑treated neutrophil group compared with the NS and control groups (P<0.01), which was also associated with the BCG dose (P<0.01). The gross tumor volume percentage in BCG‑treated neutrophils from tumor‑bearing mice (BCGT group) was significantly lower in comparison with that in the NS‑treated neutrophils from tumor‑bearing mice (NST group; P<0.05). In addition, the survival rate of tumor‑bearing mice was higher in the BCGT group compared with the NST group (P<0.05), while more BCG‑treated neutrophils from tumor‑bearing mice were infiltrated in the MethA tumor (P<0.01). In conclusion, BCG‑treated neutrophils were observed to enhance the antitumor efficacy and extend the life span of mice.
Molecular cloning and expression of visinin-like protein 1 from Gekko japonicus spinal cord
2010
Biologia (Bratislava)
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Northern blotting Northern blotting was performed as described by Wang et al. (2008) . ...
The regeneration bud appears at 2 weeks after tail amputation and begins to elongate in the posterior direction (Wang et al. 2009 ). ...
doi:10.2478/s11756-009-0225-1
fatcat:3ro7pajno5aaljtiukilygo7sq
Theoretical analysis of new optical microcavity
2018
Wuli xuebao
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Optical microcavity can confine light into a small volume by resonant recirculation. Devices based on optical microcavities are already indispensable for a wide range of applications and studies. They not only apply to traditional optics, but also have broad application prospects in quantum information and integrated optoelectronic chips. In quantum optical devices, microcavity can cause atoms or quantum dots to emit spontaneous photons in a desired direction or can provide an environment where
doi:10.7498/aps.67.20180067
fatcat:blwxnmqmc5fc7jtgayy7q6n7wq
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... dissipative mechanisms such as spontaneous emission are overcome so that quantum entanglement of radiation and matter is possible. For better application in quantum communication, optical microcavity needs to have a high quality factor and a low mode volume. Considering the beam coupling, spot shape and experimental production and others, the Fabry-Perot (F-P) microcavity has been widely applied to the field of optoelectronics. However, the Q-factor of the F-P microcavity is generally low, and the mode volume is large, so it needs to be improved. In addition, high Q-factor microcavity can also play a large role in detecting particles and biological macromolecules. In this paper, through the theory of wave optics, the eigenmodes of a new type of cone-top cylindrical optical micro-cavity are analyzed, and the resonant wavelength expression of the resonant cavity is obtained. We discuss the effects of the top mirror angle on the resonator performance and application of COMSOL simulation software to verify the proposed cone-top cylindrical microcavity. The optimized design and simulation results show that the quality factor of the new resonator can be increased by 22.4% to 49928.5 and the effective mode volume of the resonator can be reduced by 47.8% compared with the traditional parallel resonator. In this case, the corresponding new cavity length is 4.51 µm and the diameter is 3.13 µm. In this article its fabrications are also discussed.
Immunoglobulin G Expression in Human Sperm and Possible Functional Significance
2016
Scientific Reports
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Immunoglobulin G (IgG), the major molecule of the immune system, which was traditionally thought to be produced by differentiated B-lymphocytes, had recently been found in non-immune cells including spermatozoa of rabbit testis. To study if human sperms could produce IgG that might play a role in fertilization, we employed immunofluorescent staining, Western blot, in situ hybridization, RT-PCR (reverse transcription polymerase chain reaction) and immunoelectron microscope and found that human
doi:10.1038/srep20166
pmid:26833114
pmcid:PMC4735602
fatcat:4hb2ckn2ofhwpdf2hizimh5lhy
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... erms were capable of synthesizing IgG. IgG protein and mRNA were detected in the cytoplasm, mainly the neck region of the sperm and IgG immunoreactivity was found to cover the entire sperm cell. The essential enzymes necessary for IgG synthesis and class switching, RAG1 (recombination activating gene 1), RAG2 (recombination activating gene 2) and AID (activation-induced cytidine deaminase), were also detected in the sperm cells. Furthermore, we found that anti-IgG antibody could inhibit sperm from penetrating Zona-free hamster egg with statistical significance. These discoveries suggested that immunoglobulin G could be produced by human sperms and it might play a role during fertilization. Recent discovery of IgG expression in non-lymphocytes has challenged the classical concept that this important immune molecule can only be synthesized and released by mature B lymphocytes and plasma cells. It is now known that cell types that can synthesize IgG include cancer cells and normal cells such as hepatocytes, placental trophoblasts, neurons, cells in the eye and the testis 1-8 . The presence of IgG and IgA in mammalian sperm was reported in a few studies including the detection of immunoglobulin related transcripts in a single human sperm 9-13 . IgG was also abundantly detected in fluid from the distal end of the ductus epididymidis, accessory glands and spermatozo cells in rabbits 14, 15 . Previous studies based on the results of RT-PCR seemed to suggest that neonatal Fc receptor (FcRn) was shown to express in the reproductive system, where IgG can be transported across the reproductive epithelium via such receptors 16 . These findings suggested that IgG might be expressed by human sperm, but direct evidence to support this assumption has not been forthcoming. The functions of IgG produced by non-lymphocytes have been investigated. Tumor-derived IgG is thought to promote the growth and survival of cancer cells 7 . In brain, IgG might act as a self-protective factor via a mechanism of enhancing microglial endocytosis and release of tumor necrosis factor-α (TNF-α ) or by neutralizing complement factors 17,18 . Margni et al. isolated a special type of IgG from pregnancy women's sera 19 . These IgG molecules had an aberrant high mannose-type oligosaccharide residue in only one of its Fab fragments and were named asymmetric IgG 20,21 . The oligosaccharide residue in Fab fragment could bind to concanavalin A with which the asymmetric IgG can be separated from the symmetric IgG that had no oligosaccharide on its Fab 20,22 . Further studies suggested that asymmetrically glycosylated IgG failed to trigger immune effector mechanisms, and played a protective role in fetal survival 19, 21, 23 . Similar phenomenon was observed in ovarian and endometrial cancer patients by
Smoking habits and benign prostatic hyperplasia
2016
Medicine
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Previous studies have warned against the promoting effects of cigarette smoking on benign prostatic hyperplasia (BPH). In contrast, some have argued that smoking confers a protective effect regarding BPH, while others have observed an aggravated effect. Thus, we performed this meta-analysis to determine whether cigarette use is associated with BPH risk. To identify articles from observational studies of relevance, a search was performed concurrent to March 21, 2016, on PubMed, Web of Science,
doi:10.1097/md.0000000000004565
pmid:27512883
pmcid:PMC4985338
fatcat:wah46wfly5dxheehqqqoqvqtum
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... chrane, EBSCO, and EMBASE databases. Random-effect model, according to the heterogeneity, was calculated to reveal the relative risks (RRs) and corresponding 95% confidence intervals (CIs). Eight articles were included in this meta-analysis, representing data for 44,100 subjects, of which 5221 (11.8%) had BPH as defined according to the criteria. Seven reports are concerned with analysis between nonsmokers and ex-smokers, in which no significant difference was observed (RR = 0.99, 95% CI 0.94-1.05). Another meta-analysis of 7 studies indicated an observable trend, but without significant difference between groups of nonsmokers and current smokers (RR = 1.17, 95% CI 0.98-1.41). Between groups of heavy (6 articles; RR = 1.02, 95% CI 0.84-1.24) and light smokers (5 articles; RR = 0.90, 95% CI 0.71-1.15), again no significant difference appears. Finally, we combined individuals as never-smokers and ever-smokers and still found no significant difference between the 2 groups of patients (RR = 1.03, 95% CI 0.92-1.15). Sensitivity analysis was displayed and confirmed the stability of the present results. Combined evidence from observational studies shows no significant association between cigarette smoking and BPH risk, either for ex-smokers or for current smokers. The trend of elevated BPH risk from smoking was observed only in current smokers compared with nonsmokers, while marginal significance was observed in comparing ever-smokers with never-smokers in operative patients with BPH. Abbreviations: BPH = benign prostatic hyperplasia, CI = confidence interval, DHT = dihydrotestosterone, LUTS = lower urinary tract symptoms, NOS = Newcastle-Ottawa Scale, RR = relative risk.
HMGB1 Protein Does Not Mediate the Inflammatory Response in Spontaneous Spinal Cord Regeneration
2013
Journal of Biological Chemistry
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Northern Blotting-Northern blotting was performed as described by Wang et al. (39) . ...
EXPERIMENTAL PROCEDURES Animals-Adult G. japonicus were used as described by Wang et al. (43) . ...
doi:10.1074/jbc.m113.463810
pmid:23649623
pmcid:PMC3689963
fatcat:mnhbjkfkbfcu7cwrkniqpw3ic4
DeepProf: Performance Analysis for Deep Learning Applications via Mining GPU Execution Patterns
[article]
2017
arXiv
pre-print
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Deep learning applications are computation-intensive and often employ GPU as the underlying computing devices. Deep learning frameworks provide powerful programming interfaces, but the gap between source codes and practical GPU operations make it difficult to analyze the performance of deep learning applications. In this paper, through examing the features of GPU traces and deep learning applications, we use the suffix tree structure to extract the repeated patten in GPU traces. Performance
arXiv:1707.03750v1
fatcat:4qclasz5krgcfh4a25hhsu6rdu
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... ysis graphs can be generated from the preprocessed GPU traces. We further present DeepProf, a novel tool to automatically process GPU traces and generate performance analysis reports for deep learning applications. Empirical study verifies the effectiveness of DeepProf in performance analysis and diagnosis. We also find out some interesting properties of Tensorflow, which can be used to guide the deep learning system setup.
Disrupting tumour vasculature and recruitment of aPDL1-loaded platelets control tumour metastasis
2021
Nature Communications
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AbstractAlthough therapies of cancer are advancing, it remains challenging for therapeutics to reach the sites of metastasis, which accounts for majority of cancer associated death. In this study, we have developed a strategy that guides an anti-programmed cell death-ligand 1 (aPDL1) antibody to accumulate in metastatic lesions to promote anti-tumour immune responses. Briefly, we have developed a combination in which Vadimezan disrupts tumour blood vessels of tumour metastases and facilitates
doi:10.1038/s41467-021-22674-3
pmid:33986264
fatcat:v3z672k4pjg4flbm6llrpqpni4
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... e recruitment and activation of adoptively transferred aPDL1-conjugated platelets. In situ activated platelets generate aPDL1-decorated platelet-derived microparticles (PMP) that diffuse within the tumour and elicit immune responses. The proposed combination increases 10-fold aPDL1 antibody accumulation in lung metastases as compared to the intravenous administration of the antibody and enhances the magnitude of immune responses leading to improved antitumour effects.
Online Measurement Error Detection for the ElectronicTransformer in a Smart Grid
2021
Energies
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With the development of smart power grids, electronic transformers have been widely used to monitor the online status of power grids. However, electronic transformers have the drawback of poor long-term stability, leading to a requirement for frequent measurement. Aiming to monitor the online status frequently and conveniently, we proposed an attention mechanism-optimized Seq2Seq network to predict the error state of transformers, which combines an attention mechanism, Seq2Seq network, and
doi:10.3390/en14123551
fatcat:cnpsjhe6b5d7nlklkep4jqkyoy
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... ectional long short-term memory networks to mine the sequential information from online monitoring data of electronic transformers. We implemented the proposed method on the monitoring data of electronic transformers in a certain electric field. Experiments showed that our proposed attention mechanism-optimized Seq2Seq network has high accuracy in the aspect of error prediction.
Fatty Acid Metabolism Reprogramming in Advanced Prostate Cancer
2021
Metabolites
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Prostate cancer (PCa) is a carcinoma in which fatty acids are abundant. Fatty acid metabolism is rewired during PCa development. Although PCa can be treated with hormone therapy, after prolonged treatment, castration-resistant prostate cancer can develop and can lead to increased mortality. Changes to fatty acid metabolism occur systemically and locally in prostate cancer patients, and understanding these changes may lead to individualized treatments, especially in advanced,
doi:10.3390/metabo11110765
pmid:34822423
pmcid:PMC8618281
fatcat:opbw7vdi5jghrb2kvzxahspp5u
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... prostate cancers. The fatty acid metabolic changes are not merely reflective of oncogenic activity, but in many cases, these represent a critical factor in cancer initiation and development. In this review, we analyzed the literature regarding systemic changes to fatty acid metabolism in PCa patients and how these changes relate to obesity, diet, circulating metabolites, and peri-prostatic adipose tissue. We also analyzed cellular fatty acid metabolism in prostate cancer, including fatty acid uptake, de novo lipogenesis, fatty acid elongation, and oxidation. This review broadens our view of fatty acid switches in PCa and presents potential candidates for PCa treatment and diagnosis.
Recognize the role of CD146/MCAM in the osteosarcoma progression: an in vitro study
2021
Cancer Cell International
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Background Osteosarcoma (OS) is a common malignant bone tumor with poor prognosis. We previously reviewed that CD146 is correlated with multiple cancer progression, while its impact on OS is currently not systematically studied. Methods MG63 was transfected with lentivirus to express CD146 ectopically, and anti-CD146 neutralizing antibody ab75769 was used to inhibit 143B. Cyclic migration of MG63 and co-culture between MG63 and 143B were used to explore the role of OS malignancy in CD146
doi:10.1186/s12935-021-02006-7
pmid:34103063
fatcat:7soksnivpvadvfh2ch5pq42gzu
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... ion. The effect of OS cell medium (CM) on endothelium behaviors was assessed, and the expression changes of CD146 before and after co-culture of endothelium and OS were evaluated. Finally, the expression of CD146 in OS was detected under different culture conditions, including hyperoxia, low oxygen, high glucose and low glucose conditions. Results CD146 promoted the colony formation, migration, invasion and homotypic adhesion of OS cells, and reducing the concentration of soluble CD146 in the OS medium inhibited the proliferation, migration and lumen formation of the cultured endothelium. However, CD146 did not affect the adhesion between OS and endothelium, nor did co-culture of both sides affect the CD146 expression. Similarly, the proliferation, migration and CD146 expression of MG63 remained unchanged after many cycles of migration itself, as did its co-culture with 143B for expressing CD146. In addition, we also showed that high glucose promoted the expression of CD146 in OS, while hypoxia had the opposite effect. Conclusions These findings demonstrate that CD146 promotes OS progression by mediating pro-tumoral and angiogenic effects. Thus, CD146 could be a potential therapeutic target for OS, especially for OS patients with diabetes.
Coexisting Ferromagnetic-antiferromagnetic Phase and Manipulation in Magnetic Topological Insulator MnBi4Te7
[article]
2021
arXiv
pre-print
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Huan Wang is supported by the Outstanding Innovative Talents Cultivation Funded
Programs 2020 of Renmin University of China. Z. H. ...
Gong, Y.; Guo, J.; Li, J.; Zhu, K.; Liao, M.; Liu, X.; Zhang, Q.; Gu, L.; Tang, L.; Feng, X.; Zhang, D.; Li, W.; Song, C.;
Wang, L.; Yu, P.; Chen, X.; Wang, Y.; Yao, H.; Duan, W.; Xu, Y.; Zhang, S. ...
arXiv:2112.02320v1
fatcat:vdgch6t3efhfzpbqa6axhybnqi
Versatile Graphene Quantum Dots with Tunable Nitrogen Doping
2013
Particle & particle systems characterization
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A regulatory circuitry comprising TP53, miR-29 family, and SETDB1 in non-small cell lung cancer
2018
Bioscience Reports
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Lung cancer is a malignant tumor with high fatality rate and causes great harm to human economic life. Non-small cell lung cancer (NSCLC) is the most common type of lung cancer. With the rapid development of epigenetic study in the last decade, the understanding of the pathogenesis of lung cancer and the development of personalized treatment of lung cancer are picking up pace. Previous studies showed that miR-29 family members (miR-29s; miR-29a, -29b, and -29c) are down-regulated in most human
doi:10.1042/bsr20180678
pmid:30054425
fatcat:kint35kamvdrxkdsz4nvdijdse
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... ancers, including NSCLC, but their biological roles in tumorigenesis and their regulation mechanism are still not fully elucidated. Herein, we reported that the miR-29a, -29b and, -29c were coincidently down-regulated in NSCLC, and the histone H3K9 methyltransferase SET domain, bifurcated 1 (SETDB1) was directly targetted by miR-29s. Moreover, SETDB1 negatively regulated the expression of TP53 and overexpression of SETDB1 down-regulating the expression of miR-29s, while TP53 positively regulated the expression of miR-29s and overexpression of TP53 down-regulated the expression of SETDB1. On the other side, as a downstream target of TP53, the H3K9 methyltransferase Suv39h1 was also down-regulated by miR-29s via up-regulating TP53 expression. The further detection of H3K9 methylation status after changes in miR-29s expression revealed that they negatively regulated the levels of H3K9 diand trimethylation in NSCLC. Collectively, our findings highlight a TP53/miR-29s/SETDB1 regulatory circuitry and assign a role of H3K9 methylation regulator to miR-29s, which may be a potential therapeutic target in the treatment of NSCLC.
Direct acetoxylation and etherification of anilides using phenyliodine bis(trifluoroacetate)
2011
Organic and biomolecular chemistry
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