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Digital Imaging Mass Spectrometry

Casimir Bamberger, Uwe Renz, Andreas Bamberger
2011 Journal of the American Society for Mass Spectrometry  
This imaging mass spectrometer has a spatial resolving power at the specimen of (84±35)μm with a mass resolution of 45 and locates atoms or organic compounds on a surface area up to~2 cm 2 .  ...  Most mass spectrometric imagers acquire high mass resolution spectra spot-by-spot and thereby scan the object's surface. Thus, imaging is slow and image reconstruction remains cumbersome.  ...  The authors are grateful to Professor Rüdiger Brenn for providing main parts of the time of flight spectrometer. The help of Dr.  ... 
doi:10.1007/s13361-011-0120-1 pmid:21953049 fatcat:7lau6yhgzngfbpdax3oi4ok7ru

Digital Imaging Mass Spectrometry [article]

Casimir Bamberger, Uwe Renz, Andreas Bamberger
2011 arXiv   pre-print
Most mass spectrometric imagers acquire high mass resolution spectra spot-by-spot and thereby scan the object's surface. Thus, imaging is slow and image reconstruction remains cumbersome.  ...  This imaging mass spectrometer has a spatial resolving power at the specimen of (84\pm35) \mu m with a mass resolution of 45 and locates atoms or organic compounds on a surface area up to ~2 cm2.  ...  The advantage is the parallel acquisition of mass spectrometric data from an extended sample surface [9] .  ... 
arXiv:1103.1981v1 fatcat:q7jmgurdtjhy5ju3cgq6hndsp4

High Resolution Mass Spectrometric Imaging for Single Cell Metabolic Analysis

Jae Kyoo Lee, Samuel Kim, Hong Gil Nam, Richard N. Zare
2014 Biophysical Journal  
by reducing the amount of data that needs to be acquired to create a high-quality image.  ...  Our results demonstrate that the improved HPSICM technique successfully visualizes the extremely convoluted surface of stereocilia in live auditory hair cells at a high resolution and a faster speed.  ...  Unfortunately, no dynamic data at high spatial resolution could be acquired so far on the cytoskeleton of live cells, and the mechanical heterogeneity at the subcellular level remains unknown.  ... 
doi:10.1016/j.bpj.2013.11.4377 fatcat:cav5dz7oxvga5ppmrn6lseeddm

Tools and strategies for visualization of large image data sets in high-resolution imaging mass spectrometry

Ivo Klinkert, Liam A. McDonnell, Stefan L. Luxembourg, A. F. Maarten Altelaar, Erika R. Amstalden, Sander R. Piersma, Ron M. A. Heeren
2007 Review of Scientific Instruments  
Using different data processing strategies for visualization, it enables fast mass spectrometric imaging of large surfaces at high-spatial resolution and thus aids in the understanding of various diseases  ...  Therefore, in the context of the virtual laboratory of enhanced science, software tools are developed to handle mass spectrometry data sets.  ...  This data type is characterized by a lower spatial resolution but a high mass resolution. This high mass resolution is employed to visualize different component ͑m / z͒ distributions.  ... 
doi:10.1063/1.2737770 pmid:17552834 fatcat:omdl2pyuyzbelfj3mmftulcou4

Challenges and recent advances in mass spectrometric imaging of neurotransmitters

Erin Gemperline, Bingming Chen, Lingjun Li
2014 Bioanalysis  
Background Mass spectrometric imaging (MSI) shows great promise for biological analyses because it allows for molecular analysis of tissue while retaining information about the spatial distribution of  ...  During an MSI experiment, a multitude of mass spectra are collected from a tissue slice in a predefined raster, resulting in a 2D distribution map for each mass measured.  ...  Data processing • There have been several recent advances for improved mass resolution, spatial resolution, acquisition time and data processing.  ... 
doi:10.4155/bio.13.341 pmid:24568355 pmcid:PMC4159677 fatcat:vlbis4dhsnfcfeivcnkg77lgvi

Ultrafast High-Resolution Mass Spectrometric Finger Pore Imaging in Latent Finger Prints

Christian Elsner, Bernd Abel
2014 Scientific Reports  
Here we show the potential of fast finger pore imaging (FPI) in latent finger prints employing LD-MSI without a classical matrix in a high-spatial resolution mode.  ...  Compared to other techniques, mass spectrometric (MS) methods offer the major advantage of chemically specific detection and identification of various endogenous compounds such as lipids and exogenous  ...  Partial funding of this work by the Deutsche Forschungsgemeinschaft through SFB 755 and the Leibniz-Gemeinschaft is gratefully acknowledged.  ... 
doi:10.1038/srep06905 pmid:25366032 pmcid:PMC4219163 fatcat:grcqgvbdmrazrh6antybrso6qq

Next-generation technologies for spatial proteomics: Integrating ultra-high speed MALDI-TOF and high mass resolution MALDI FTICR imaging mass spectrometry for protein analysis

Jeffrey M. Spraggins, David G. Rizzo, Jessica L. Moore, Michael J. Noto, Eric P. Skaar, Richard M. Caprioli
2016 Proteomics  
High spatial resolution MALDI-TOF protein images of rat brain tissue and cystic fibrosis lung tissue were acquired at image acquisition rates >25 pixels/s.  ...  MALDI imaging mass spectrometry is a powerful analytical tool enabling the visualization of biomolecules in tissue.  ...  Acknowledgments The authors wish to acknowledge the support of grants from the NIH/NIGMS (5 P41 GM103391-04 and 5R01 GM058008) and the National Institutes of Health  ... 
doi:10.1002/pmic.201600003 pmid:27060368 pmcid:PMC5117945 fatcat:vyliy7rm6fafvogeht5664wxvu

Mass spectrometric analysis of spatio-temporal dynamics of crustacean neuropeptides

Chuanzi OuYang, Zhidan Liang, Lingjun Li
2015 Biochimica et Biophysica Acta - Proteins and Proteomics  
Here, we focus our discussion on recent advances in the use of MS-based techniques to map neuropeptides in spatial domain and monitoring their dynamic changes in temporal domain.  ...  Over the past several years, mass spectrometry (MS)-based strategies have revolutionized the discovery of neuropeptides in numerous model organisms, especially in decapod crustaceans.  ...  Mass spectrometric imaging-the spatial probe of crustacean neuropeptides 2.1 Introduction to mass spectrometric imaging Immunohistochemical (IHC) staining has long been a powerful tool for visualizing  ... 
doi:10.1016/j.bbapap.2014.10.023 pmid:25448012 pmcid:PMC4418951 fatcat:mzcft6cyjrbr3dr6kyokyqwcvi

Atomic Force Microscopy Reveals the Structure and Dynamics of the Cell Cortex

Frederic Eghiaian, Simon Scheuring
2014 Biophysical Journal  
by reducing the amount of data that needs to be acquired to create a high-quality image.  ...  Our results demonstrate that the improved HPSICM technique successfully visualizes the extremely convoluted surface of stereocilia in live auditory hair cells at a high resolution and a faster speed.  ...  Unfortunately, no dynamic data at high spatial resolution could be acquired so far on the cytoskeleton of live cells, and the mechanical heterogeneity at the subcellular level remains unknown.  ... 
doi:10.1016/j.bpj.2013.11.4378 fatcat:b2jqanh45vdrzdmeeeyhfkwdfq

A Novel Platform for Simultanoues Mechanical Stimulation and Characterization of Single Cells Based on Dielectric Elastomers and Atomic Force Microscopy

Roberto Raiteri, Natalia Becerra, Mariateresa Tedesco, Christopher Ward, Federico Carpi, Gabriele Frediani, Pasquale Vena
2014 Biophysical Journal  
by reducing the amount of data that needs to be acquired to create a high-quality image.  ...  Our results demonstrate that the improved HPSICM technique successfully visualizes the extremely convoluted surface of stereocilia in live auditory hair cells at a high resolution and a faster speed.  ...  Unfortunately, no dynamic data at high spatial resolution could be acquired so far on the cytoskeleton of live cells, and the mechanical heterogeneity at the subcellular level remains unknown.  ... 
doi:10.1016/j.bpj.2013.11.4376 fatcat:ocgyww34crfv3ms6h2k76wcp2y

Compressed Sensing Based Atomic Force Microscopy

Brian D. Maxwell, Sean B. Andersson
2014 Biophysical Journal  
by reducing the amount of data that needs to be acquired to create a high-quality image.  ...  Our results demonstrate that the improved HPSICM technique successfully visualizes the extremely convoluted surface of stereocilia in live auditory hair cells at a high resolution and a faster speed.  ...  Unfortunately, no dynamic data at high spatial resolution could be acquired so far on the cytoskeleton of live cells, and the mechanical heterogeneity at the subcellular level remains unknown.  ... 
doi:10.1016/j.bpj.2013.11.4375 fatcat:frmpp7ylgzdidcgbjt27fjvakm

High-Speed Tandem Mass Spectrometric in Situ Imaging by Nanospray Desorption Electrospray Ionization Mass Spectrometry

Ingela Lanekoff, Kristin Burnum-Johnson, Mathew Thomas, Joshua Short, James P. Carson, Jeeyeon Cha, Sudhansu K. Dey, Pengxiang Yang, Maria C. Prieto Conaway, Julia Laskin
2013 Analytical Chemistry  
acquisition enabled simultaneous imaging and identification of a large number of metabolites and lipids from 92 selected m/z windows (±1 Da) with a spatial resolution of better than 150 μm.  ...  Molecular ions and their corresponding fragments, separated by high-resolution mass analysis, were assigned on the basis of accurate mass measurement.  ...  Department of Energy (DOE) under Contract DE-AC05-76RL01830.  ... 
doi:10.1021/ac401760s pmid:24040919 pmcid:PMC3867692 fatcat:fxltwt65wzatzalubbvm6k4dey

Fast, High Resolution Mass Spectrometry Imaging Using a Medipix Pixelated Detector

Julia H. Jungmann, Luke MacAleese, Ronald Buijs, Frans Giskes, Ad de Snaijer, Jan Visser, Jan Visschers, Marc J.J. Vrakking, Ron M.A. Heeren
2010 Journal of the American Society for Mass Spectrometry  
A detailed evaluation of key performance criteria such as spatial resolution, acquisition speed, and data handling is presented.  ...  In mass spectrometry imaging, spatial resolution is pushed to its limits with the use of ion microscope mass spectrometric imaging systems.  ...  Acknowledgments The authors acknowledge that this work is part of the research program of the "Stichting voor Fundamenteel Onderzoek der Materie (FOM)," which is financially supported by the "Nederlandse  ... 
doi:10.1016/j.jasms.2010.08.014 pmid:20971654 fatcat:2t2tua74xngy3bmxp7hn72fzii

Spatial profiling invertebrate ganglia using MALDI MS

Rebecca Kruse, Jonathan V. Sweedler
2003 Journal of the American Society for Mass Spectrometry  
Currently, the ability to obtain high quality mass spectrometric images depends on sample preparation protocols that often result in limited spatial resolution.  ...  Recent advances in instrumentation for MS have resulted in instruments capable of achieving several micron spatial resolution while acquiring high-resolution mass spectra.  ...  Robert Fuller) to function with Voyager 5.1/Data Explorer 4.0 acquisition and processing software. They acknowledge Jason Page for assistance with electrospray matrix deposition.  ... 
doi:10.1016/s1044-0305(03)00288-5 pmid:12837597 fatcat:atpcyrf2znb3jk5cbbkj6fbmum

High-throughput proteomics using matrix-assisted laser desorption/ ionization mass spectrometry

Rainer Cramer, Johan Gobom, Eckhard Nordhoff
2005 Espert Review of Proteomics  
It can be relatively inexpensive and extremely fast with regard to data acquisition.  ...  . • For optimal data exploitation and high-throughput proteomics, it is essential to develop advanced bioinformatic tools for data management and analysis.  ...  Acknowledgements The authors thank Richard Jacob for the acquisition of the spectra in FIGURE 1.  ... 
doi:10.1586/14789450.2.3.407 pmid:16000086 fatcat:b7j2hophrvbazntaftghwbgxua
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