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Heart and skeletal muscle inflammation (HSMI) disease diagnosed on a British Columbia salmon farm through a longitudinal farm study

Emiliano Di Cicco, Hugh W. Ferguson, Angela D. Schulze, Karia H. Kaukinen, Shaorong Li, Raphaël Vanderstichel, Øystein Wessel, Espen Rimstad, Ian A. Gardner, K. Larry Hammell, Kristina M. Miller, Pierre Boudinot
2017 PLoS ONE  
Our analysis showed evidence of HSMI histopathological lesions over an 11-month timespan, with the prevalence of lesions peaking at 80-100% in sampled fish, despite mild clinical signs with no associated  ...  Heart and skeletal muscle inflammation (HSMI) is an emerging disease of marine-farmed Atlantic Salmon (Salmo salar), first recognized in 1999 in Norway, and later also reported in Scotland and Chile.  ...  farm mortality and environmental data, and farm technicians for aiding in the sampling process on the farms.  ... 
doi:10.1371/journal.pone.0171471 pmid:28225783 pmcid:PMC5321275 fatcat:4bcandzevbe4tjyda26werqxoi

Reproductive age-associated fibrosis in the stroma of the mammalian ovary

Shawn M Briley, Susmita Jasti, Jennifer M McCracken, Jessica E Hornick, Barbara Fegley, Michele T Pritchard, Francesca E Duncan
2016 Reproduction  
However, if homeostasis between synthesis and degradation of extracellular matrix (ECM) components is altered, fibrosisor the excess accumulation of ECM -can disrupt tissue architecture and function.  ...  Several organs, including the heart, lung and kidney, exhibit age-associated fibrosis.  ...  K Woodruff for allowing us to use the LC-Polscope in her laboratory; and Dr Dale Abrahamson for useful discussions.  ... 
doi:10.1530/rep-16-0129 pmid:27491879 pmcid:PMC4979755 fatcat:hbcc2s2ya5h7nmiwi4ahbb5eqq

The Proton-Sensing GPR4 Receptor Regulates Paracellular Gap Formation and Permeability of Vascular Endothelial Cells

Elizabeth A. Krewson, Edward J. Sanderlin, Mona A. Marie, Shayan Nik Akhtar, Juraj Velcicky, Pius Loetscher, Li V. Yang
2020 iScience  
Genetic knockout and pharmacologic inhibition of GPR4 alleviate tissue inflammation. These results suggest GPR4 is a pro-inflammatory receptor and could be targeted for therapeutic intervention.  ...  GPR4 is a pH-sensing G protein-coupled receptor highly expressed in vascular endothelial cells and can be activated by protons in the inflamed tissue microenvironment.  ...  Cells were then washed in DPBS and mounted with DAPI-containing Vectashield mounting media (Vector Laboratories, H-1200, Burlingame, CA, USA).  ... 
doi:10.1016/j.isci.2020.100848 pmid:32058960 pmcid:PMC6997876 fatcat:x4oyvrbwn5ewlpwuynvcmnl3w4

Group I metabotropic glutamate receptors generate two types of intrinsic membrane oscillations in hippocampal oriens/alveus interneurons

Gubbi Govindaiah, Young-Jin Kang, Hannah Elisabeth Smashey Lewis, Leeyup Chung, Ethan M. Clement, Lazar John Greenfield, Edgar Garcia-Rill, Sang-Hun Lee
2018 Neuropharmacology  
The selective mGluR1 antagonist (S)-(+)-α-Amino-4-carboxy-2-methylbenzeneacetic acid (LY367385) reduced the slow oscillations, and the selective mGluR5 antagonist 2-methyl-6-(phenylethynyl)pyridine hydrochloride  ...  ., CA1), and express group I metabotropic glutamate receptors (mGluRs), specifically mGluR1 and mGluR5.  ...  To visualize the axonal and dendritic arbors, the biocytin-filled cells were subsequently treated with a Vectastain ABC kit (Vector Laboratories, Burlingame, CA, USA; PK-4000), and a DAB peroxidase substrate  ... 
doi:10.1016/j.neuropharm.2018.06.035 pmid:29964095 pmcid:PMC7007965 fatcat:ppzde553nrccxblifwa3p4exvi

Neural Damage in Experimental Trypanosoma brucei gambiense Infection: Hypothalamic Peptidergic Sleep and Wake-Regulatory Neurons

Claudia Laperchia, Yuan-Zhong Xu, Dieudonné Mumba Ngoyi, Tiziana Cotrufo, Marina Bentivoglio
2018 Frontiers in Neuroanatomy  
Cell counts and evaluation of OX fiber density were performed at 4 and 8 weeks post-infection, when parasites had entered the brain parenchyma from the periphery.  ...  A significant decrease of OX neurons (about 44% reduction) and MCH neurons (about 54% reduction) was found in the lateral hypothalamus and perifornical area at 8 weeks in T. b. gambiense-infected M. natalensis  ...  , Burlingame, CA, USA) for 1 h and with 3,3 -diaminobenzidine (DAB) as chromogen.  ... 
doi:10.3389/fnana.2018.00013 pmid:29535612 pmcid:PMC5835115 fatcat:gsovy4gmdfgoxmzhuxirn4wsya

The ATF6β-calreticulin axis protects hippocampal neurons against endoplasmic reticulum stress and excitotoxicity [article]

Dinh Thi Nguyen, Thuong Manh Le, Tsuyoshi Hattori, Mika Takarada-Iemata, Hiroshi Ishii, Jureepon Roboon, Takashi Tamatani, Takayuki Kannon, Kazuyoshi Hosomichi, Atsushi Tajima, Shusuke Taniuchi, Masato Miyake (+4 others)
2021 bioRxiv   pre-print
Analysis using cultured hippocampal neurons revealed that ATF6β deficiency reduced Ca2+ stores in the ER and enhanced ER stress-induced death, which was rescued by ATF6β, calreticulin, Ca2+-modulating  ...  reagents such as BAPTA-AM and 2-APB, and ER stress inhibitor salubrinal.  ...  Danvers, MA, USA; 1:500) and c-Fos (PC05; Merck; 1:200). Nuclei were 18 visualized with 4',6-diamidino-2-phenylindole (DAPI; Sigma).  ... 
doi:10.1101/2021.02.01.429116 fatcat:u652kyb3hng7xe4g3zamokkpxm

Comparison of the Transcriptomes of Long-Term Label Retaining-Cells and Control Cells Microdissected from Mammary Epithelium: An Initial Study to Characterize Potential Stem/Progenitor Cells

Ratan K. Choudhary, Robert W. Li, Christina M. Evock-Clover, Anthony V. Capuco
2013 Frontiers in Oncology  
Conclusion: Our data suggest that LREC in the basal epithelial layer are enriched for MaSC, as these cells showed increased expression of genes that reflect stem cell attributes; whereas LREC in suprabasal  ...  Enriched expression of genes in LRECb was associated with stem cell attributes and identified WNT, TGF-β, and MAPK pathways of self renewal and proliferation.  ...  February 2013 | Volume 3 | Article 21 | 4 February 2013 | Volume 3 | Article 21 | 5 February 2013 | Volume 3 | Article 21 | 6 February 2013 | Volume 3 | Article 21 | 7 February 2013  ... 
doi:10.3389/fonc.2013.00021 pmid:23423481 pmcid:PMC3573348 fatcat:eyeovjdzjrflhah3d3jubx7vha

Elevated Expression of Serotonin 5-HT2A Receptors in the Rat Ventral Tegmental Area Enhances Vulnerability to the Behavioral Effects of Cocaine

David V. Herin, Marcy J. Bubar, Patricia K. Seitz, Mary L. Thomas, Gilbert R. Hillman, Yevgeniya I. Tarasenko, Ping Wu, Kathryn A. Cunningham
2013 Frontiers in Psychiatry  
Immunohistochemical analysis confirmed expression of Flag and overexpression of the 5-HT 2A R protein.  ...  These data indicate that the vulnerability of adult male rats to hyperactivity induced by cocaine is enhanced following increased levels of expression of the 5-HT 2A R in the VTA and suggest that the 5  ...  The 5-HT 2A R cDNA and RN46A cells were kindly provided by Dr. J. Liu (University of Cincinnati) and Dr. Scott Whittemore (University of Miami), respectively. The authors would also like to thank Dr.  ... 
doi:10.3389/fpsyt.2013.00002 pmid:23390419 pmcid:PMC3565455 fatcat:jpz2pr3htzhkba2ol3yu3l6iyq

Vildagliptin preserves the mass and function of pancreatic β cells via the developmental regulation and suppression of oxidative and endoplasmic reticulum stress in a mouse model of diabetes

S. Hamamoto, Y. Kanda, M. Shimoda, F. Tatsumi, K. Kohara, K. Tawaramoto, M. Hashiramoto, K. Kaku
2012 Diabetes, obesity and metabolism  
or a vehicle for 4 weeks.  ...  decreased and those related to anti-apoptosis and anti-oxidative stress was upregulated, in KK-A y mice.  ...  After another washing in TBS, they were visualized by reacting with a simple stain diaminobenzidine solution (Nichirei) or Vector Red (Vector Laboratories, Burlingam, CA, USA).  ... 
doi:10.1111/dom.12005 pmid:22950702 pmcid:PMC3558804 fatcat:xh4e553wvffhbgovofx6bqmnd4

Evidence for a Clathrin-independent mode of endocytosis at a continuously active sensory synapse

Michaela Fuchs, Johann Helmut Brandstätter, Hanna Regus-Leidig
2014 Frontiers in Cellular Neuroscience  
at rod and cone photoreceptor, and ON bipolar cell ribbon synapses with light and electron microscopy.  ...  These clusters labeled for Dynamin3, Endophilin1, and Synaptojanin1, but not for AP180, Clathrin LC, and hsc70.  ...  ACKNOWLEDGMENTS Grant sponsor: Deutsche Forschungsgemeinschaft; Grant numbers BR 1643/4-2 (to Johann Helmut Brandstätter and Hanna Regus-Leidig) and BR 1643/5-1 (to Johann Helmut Brandstätter).  ... 
doi:10.3389/fncel.2014.00060 pmid:24616664 pmcid:PMC3934443 fatcat:jd2z7de5l5btdd4ttiq2xnvj2y

An alternative miRISC targeting a coding mutation site in FOXL2 links to granulosa cell tumor [article]

Jeehyeon Bae, Eunkyoung Shin, Hanyong Jin, Dae-Shik Suh, Yongyang Luo, Hye-Jeong Ha, Tae Heon Kim, Yoonsoo Hahn, Seogang Hyun, Kangseok Lee
2020 bioRxiv   pre-print
In both patients and mouse model of AGCT, the inversely regulated variant FOXL2 abundance with the miR-1236 levels was highly correlated with malignant features of AGCT.  ...  This miR-1236-mediated selective degradation of the variant FOXL2 mRNA is preferentially conducted by a distinct miRNA-loaded RNA-induced silencing complex (miRISC) directed by the Argonaute3 (AGO3) and  ...  Wooseok Song, Hong-Man Kim, and Seunghwa Lee for helping with the primerextension and cell-migration experiments. We also thank Dr. Jae-Sung Woo for critical comments.  ... 
doi:10.1101/2020.02.18.954487 fatcat:r6sfq4crczfebnybario3m4fca

Candidate Glycoprotein Biomarkers for Canine Visceral Hemangiosarcoma and Validation Using Semi-Quantitative Lectin/Immunohistochemical Assays

Patharee Oungsakul, Eunju Choi, Alok K. Shah, Ahmed Mohamed, Caroline O'Leary, David Duffy, Michelle M. Hill, Helle Bielefeldt-Ohmann
2021 Veterinary Sciences  
Using lectin-histochemistry and immunohistochemistry (IHC) for key proteins on tissues with HSA and semi-quantitation of the signals, we demonstrate that a combination of DSA histochemistry and IHC for  ...  In order to improve early and differential diagnostic capabilities and hence, prognosis for dogs with HSA, two types of biomarker are needed: a point-of-care diagnostic biomarker and a prognostic biomarker—preferentially  ...  Acknowledgments: We thank Mark Haworth, Jane McGhie, Alana Rosenblatt, Willy Suen, and Sarah Kaye (UQ Veterinary Medical Centre and UQ-SVS Diagnostic Services) for providing fresh tissue Conflicts of  ... 
doi:10.3390/vetsci8030038 pmid:33673507 fatcat:agxrgoh2hrasphkmhdt6lbhgry

Neurological effects of inorganic arsenic exposure: altered cysteine/glutamate transport, NMDA expression and spatial memory impairment

Lucio A. Ramos-Chávez, Christian R. R. Rendón-López, Angélica Zepeda, Daniela Silva-Adaya, Luz M. Del Razo, María E. Gonsebatt
2015 Frontiers in Cellular Neuroscience  
On postnatal days (PNDs) 1, 15 and 90, the expression of membrane transporters related to GSH synthesis and glutamate transport and toxicity, such as xCT, EAAC1, GLAST and GLT1, as well as LAT1, were analyzed  ...  In humans, As species cross the placenta and are found in cord blood.  ...  D. for his assistance during the confocal analysis of brain slices. Lucio A. Ramos-Chávez received a scholar fellowship from CONACYT No. 211718 and DGEP UNAM.  ... 
doi:10.3389/fncel.2015.00021 pmid:25709567 pmcid:PMC4321597 fatcat:gcodhl7ebvgrpmzecxjxjzzija

Chronic Over-Expression of Heat Shock Protein 27 Attenuates Atherogenesis and Enhances Plaque Remodeling: A Combined Histological and Mechanical Assessment of Aortic Lesions

Charles M. Cuerrier, Yong-Xiang Chen, Dominique Tremblay, Katey Rayner, Melissa McNulty, XiaoLing Zhao, Christopher R. J. Kennedy, Jacqueline de BelleRoche, Andrew E. Pelling, Edward R. O'Brien, Ali A. Sovari
2013 PLoS ONE  
These data provide new clues as to how HSP27 may improve not only the composition of atherosclerotic lesions but potentially their stability and resilience to plaque rupture.  ...  The analysis of the aortic mechanical properties showed increased vessel stiffness in apoE 2/2 HSP27 o/e mice (41% in female, 34% in male) compare to apoE 2/2 counterparts.  ...  Antibody reactivity was detected using an ABC kit (Vector Laboratories, Burlingame, California, USA) and visualized with diaminobenzidine (DAB)/ hydrogen peroxidase as chromogenic substrate, resulting  ... 
doi:10.1371/journal.pone.0055867 pmid:23409070 pmcid:PMC3567023 fatcat:27tj63lsynbf7p3huh3tzfpoae

COSMC Is Overexpressed in Proliferating Infantile Hemangioma and Enhances Endothelial Cell Growth via VEGFR2

Jian-Jr Lee, Chia-Hua Chen, Ya-Hsin Chen, Miao-Juei Huang, John Huang, Ji-Shiang Hung, Ming-Ting Chen, Min-Chuan Huang, Surinder K. Batra
2013 PLoS ONE  
Overexpression of COSMC significantly enhanced cell growth and phosphorylation of AKT and ERK in human umbilical vein endothelial cells (HUVECs).  ...  Mechanistic investigation showed that O-glycans were present on VEGFR2 and these structures were modulated by COSMC.  ...  Cummings (the Emory University School of Medicine, Atlanta, USA) for plasmid COSMC/pcDNA3.1 and Drs.  ... 
doi:10.1371/journal.pone.0056211 pmid:23424651 pmcid:PMC3570459 fatcat:bpa4rzjypjgqnp6vosdlmzuh4i
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