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Jaesung Lee, Artit Jirapatnakul, Anthony Reeves, William Crowe, Ingrid Sarelius
2007 2007 4th IEEE International Symposium on Biomedical Imaging: From Nano to Macro  
A new feature-based tracking algorithm was developed for measuring diameter of vessels in intravital video microscopy image sequences.  ...  Testing was performed with ten synthetic image sequences with known diameters and ten intravital microscopy sequences.  ...  Typically, intravital video microscopy is used to observe blood vessels in vivo [2] ; video image sequences are recorded of the subjects and later analyzed to obtain diameter measurements.  ... 
doi:10.1109/isbi.2007.356863 dblp:conf/isbi/LeeJRCS07 fatcat:bomcswoz6zhuvg55nz4lxyyo4m

Vessel Diameter Measurement from Intravital Microscopy

Jaesung Lee, Artit C. Jirapatnakul, Anthony P. Reeves, William E. Crowe, Ingrid H. Sarelius
2009 Annals of Biomedical Engineering  
The proposed method tracks the vessel diameter throughout the entire image sequence once the diameter is marked in the first video frame.  ...  Two seed points, indicating the vessel diameter, are placed on the vessel walls and tracked throughout the entire image sequence using feature tracking algorithm.  ...  eter tracking algorithm correctly measures the vessel diameter through each intravital microscopy image sequence.  ... 
doi:10.1007/s10439-009-9666-5 pmid:19280342 pmcid:PMC2754796 fatcat:tyzljfdtrvbe7fitk7gcqdi5zm

Vessel Boundary Tracking for Intravital Microscopy Via Multiscale Gradient Vector Flow Snakes

J. Tang, S.T. Acton
2004 IEEE Transactions on Biomedical Engineering  
Due to movement of the specimen, vasodilation, and intense clutter, the intravital location of a vessel boundary from video microscopy is a difficult but necessary task in analyzing the mechanics of inflammation  ...  This paper details an active contour model for vessel boundary detection and tracking. In developing the method, two innovations are introduced.  ...  The cremaster muscle was prepared for intravital microscopy as described in [14] . Venules with diameters between 20 and 40 were observed.  ... 
doi:10.1109/tbme.2003.820374 pmid:14765704 fatcat:pufgllqrjfhu3lkdgb337h7kny

Intravital Microscopy of the Mouse Brain Microcirculation using a Closed Cranial Window

Pedro Cabrales, Leonardo J. M. Carvalho
2010 Journal of Visualized Experiments  
Local microcirculation is recorded in real time through the window using epi and fluorescence illumination, and measurements of vessels diameters and red blood cell (RBC) velocities are performed.  ...  We used for the first time the close window brain intravital microscopy to study the pial microcirculation to follow dynamic changes during the course of Plasmodium berghei ANKA infection in mice and show  ...  Figure 2 . 2 (Steps 3.1-3.5) Schematic display of the set up for intravital microscopy of the mouse pial microcirculation.  ... 
doi:10.3791/2184 pmid:21113121 pmcid:PMC3074458 fatcat:zay3e6rcqngj7motfkeuyxiure

Time-lapse imaging of disease progression in deep brain areas using fluorescence microendoscopy

Robert P J Barretto, Tony H Ko, Juergen C Jung, Tammy J Wang, George Capps, Allison C Waters, Yaniv Ziv, Alessio Attardo, Lawrence Recht, Mark J Schnitzer
2011 Nature Medicine  
for computing erythrocyte speeds and computed relationships between vessel diameters and speeds.  ...  A.C.W. developed bilateral imaging, performed neuronal imaging and contributed to the glioma experiments. Y.Z. developed and performed striatal imaging.  ...  Figure 5 5 Quantitative tracking of glioma angiogenesis in CA1 hippocampus shows tumor vessels broaden in diameter but undergo marked declines in flow speed.  ... 
doi:10.1038/nm.2292 pmid:21240263 pmcid:PMC3833825 fatcat:tfabs5y4tffzdgmckndpwaazvi

Structural adaptations in the murine colon microcirculation associated with hapten-induced inflammation

D. J Ravnic, M. A Konerding, A. Tsuda, H. T Huss, T. Wolloscheck, J. P Pratt, S. J Mentzer
2007 Gut  
Intravital microscopy of the mucosal plexus showed a greater than twofold decrease in the velocity of flow (p,0.001).  ...  Colonic blood velocimetry was performed using intravital microscopy. Results: Clinical and histological parameters suggested a peak inflammatory response at 96 h (p,0.001).  ...  The images were recorded in image stacks comprising 30 s-10 min video sequences. The image stacks were processed with standard MetaMorph filters.  ... 
doi:10.1136/gut.2006.101824 pmid:17114297 pmcid:PMC1856840 fatcat:hwwa6qz7ojhopka5ni5kokckam

Bimodal Oscillation Frequencies of Blood Flow in the Inflammatory Colon Microcirculation

Akira Tsuda, Aslihan Turhan, Moritz Konerding, Dino Ravnic, Dusan Hanidziar, Miao Lin, Steven J. Mentzer
2009 The Anatomical Record  
In contrast, metastable oscillations demonstrated a lower frequency (0.2 to 0.4 cycles/sec) and appeared to be the result of flow dynamics in vessels linked by the bridging mucosal vessels.  ...  By tracking fluorescent nanoparticles in the inflamed mucosal plexus, we identified two forms of blood flow oscillations within the inflammatory mouse colon.  ...  Image stack analysis Digital intravital microscopy images were obtained without motion artifact to facilitate subsequent processing.  ... 
doi:10.1002/ar.20767 pmid:18951508 pmcid:PMC2867241 fatcat:ozkz646cgbaqdjvwci2wf4cvty

Blood flow shapes intravascular pillar geometry in the chick chorioallantoic membrane

Grace S Lee, Nenad Filipovic, Lino F Miele, Miao Lin, Dinee C Simpson, Barry Giney, Moritz A Konerding, Akira Tsuda, Steven J Mentzer
2010 Journal of Angiogenesis Research  
The relative contribution of blood flow to vessel structure remains a fundamental question in biology.  ...  Pillars comprised 0.02 to 0.5% of the vascular system in 2-dimensional projection and were predominantly observed at vessel bifurcations.  ...  Supported in part by NIH Grant HL47078, HL75426 and HL94567  ... 
doi:10.1186/2040-2384-2-11 pmid:20609245 pmcid:PMC2911408 fatcat:e2p52ruxcjbxjekmjbzlf2yv74

Quantitative intravital two-photon excitation microscopy reveals absence of pulmonary vaso-occlusion in unchallenged Sickle Cell Disease mice

Margaret F Bennewitz, Simon C Watkins, Prithu Sundd
2014 IntraVital  
Supplemental Materials Supplemental materials may be found here:  ...  In conclusion, we report a TPE microscopy enabled intravital imaging approach that allows simultaneous visualization of leukocyte and RBC trafficking in the lungs of SCD mice.  ...  , while the capillaries have an average diameter of 6 ± 2 µm. the complete video sequences of neutrophil migration within pulmonary capillaries and the tracking video displaying neutrophils with attached  ... 
doi:10.4161/intv.29748 pmid:25995970 pmcid:PMC4435611 fatcat:huummwq7h5fk7i7rrutguy7n3q

Laser Speckle Flowmetry Method for Measuring Spatial and Temporal Hemodynamic Alterations Throughout Large Microvascular Networks

2012 Microcirculation  
Conclusions-These data demonstrate the applicability of LSF to intravital microscopy microcirculation preparations for determining both relative and absolute hemodynamics on a network-wide scale while  ...  Results-In vitro, we found that the normalized speckle intensity (NSI) versus velocity linear relationship (R 2 ≥0.97) did not vary with diameter or hematocrit and can be shifted to meet an expected operating  ...  Acknowledgments The authors would like to thank Ji Song for her expertise in cremaster muscle preparation.  ... 
doi:10.1111/j.1549-8719.2012.00197.x pmid:22591575 pmcid:PMC3434303 fatcat:rt7gnhgjwbdgfewccx6cnt7rsm

Multispectral intravital microscopy for simultaneous bright-field and fluorescence imaging of the microvasculature

Barry G. H. Janssen, Mohamadreza Najiminaini, Yan Min Zhang, Parsa Omidi, Jeffrey J. L. Carson
2021 Applied Microscopy  
This study introduces multispectral intravital video microscopy, which combines bright-field and fluorescence microscopy in a standard light microscope.  ...  Conventional light microscopes do not allow for simultaneous bright-field and fluorescent imaging. Moreover, in conventional microscopes, only one type of fluorescent label can be observed.  ...  (B), (C) and (D): Intravital microscopy image sequence of a microbead (streak) moving in the bloodstream's centerline.  ... 
doi:10.1186/s42649-021-00059-6 fatcat:4j3qvpvl6jfq5ljc5ciw2fklba

Autonomous T cell trafficking examined in vivo with intravital two-photon microscopy

M. J. Miller, S. H. Wei, M. D. Cahalan, I. Parker
2003 Proceedings of the National Academy of Sciences of the United States of America  
Fig. 2 . 2 Intravital imaging of vessels and cells in a living lymph node.  ...  In this study, we combined intravital microscopy with 2P imaging to document the motility of naïve T cells in peripheral lymph nodes in vivo.  ... 
doi:10.1073/pnas.2628040100 pmid:12601158 pmcid:PMC151387 fatcat:4cdqzoehznegtmoq3lfetybsva

Label-Free Determination of Hemodynamic Parameters in the Microcirculaton with Third Harmonic Generation Microscopy

Steffen Dietzel, Joachim Pircher, A. Katharina Nekolla, Mazhar Gull, André W. Brändli, Ulrich Pohl, Markus Rehberg, Marc van Zandvoort
2014 PLoS ONE  
Here we show that Third Harmonic Generation (THG) microscopy is a suitable tool for label-free intravital investigations of the microcirculation in widely-used physiological model systems.  ...  Blood flow was visualized and its velocity was measured in adult mouse cremaster muscle vessels, non-invasively in mouse ear vessels and in Xenopus tadpoles.  ...  In a few frames an RBC track comes closer than usual to the vessel wall.  ... 
doi:10.1371/journal.pone.0099615 pmid:24933027 pmcid:PMC4059650 fatcat:mfd4u5xeozbbbepfgjtulkrsxq

In Vivo Imaging of Mammalian Cochlear Blood Flow Using Fluorescence Microendoscopy

Ashkan Monfared, Nikolas H Blevins, Eunice L. M Cheung, Juergen C Jung, Gerald Popelka, Mark J Schnitzer
2006 Otology and Neurotology  
Present techniques for imaging cochlear microcirculation using intravital light microscopy involve extensive perturbations to cochlear structure, precluding application in human patients.  ...  We then imaged cochlear blood flow within individual vessels in an epifluorescence configuration using one-photon fluorescence microendoscopy.  ...  Intravital fluorescence microscopy is an optical imaging method that can provide detailed information on blood flow within single cochlear vessels.  ... 
doi:10.1097/01.mao.0000190708.44067.b0 pmid:16436982 pmcid:PMC2820368 fatcat:2edadcbzqfdh5ea5mkjblagyzq

Coalescent angiogenesis—evidence for a novel concept of vascular network maturation

Bianca Nitzsche, Wen Wei Rong, Andrean Goede, Björn Hoffmann, Fabio Scarpa, Wolfgang M. Kuebler, Timothy W. Secomb, Axel R. Pries
2021 Angiogenesis  
In the present study, sustained (33-h) intravital microscopy of the vasculature in the chick chorioallantoic membrane (CAM) led to the hypothesis of a novel non-sprouting mode for vessel generation, which  ...  ., the division or splitting of an existing vessel, as preferential mode of new vessel formation.  ...  in adjacent regions distribution Blood vessel diameter Homogene- Diameter increase in preferential Increased diameter in emergent Further increase in diameter in ous capillary pathway vessel established  ... 
doi:10.1007/s10456-021-09824-3 pmid:34905124 pmcid:PMC8669669 fatcat:7orpw7rrhbhqtnzyjnae3awiwi
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