Improvement of axial resolution and contrast in temporally focused widefield two-photon microscopy with structured light illumination
Biomedical Optics Express
We implemented a structured light illumination for TFM and have shown that it can effectively reject the out-of-focus scattered emission photons improving image contrast. ...
Although temporally focused wide-field two-photon microscopy (TFM) can perform depth resolved wide field imaging, it cannot avoid the image degradation due to scattering of excitation and emission photons ...
supported by NIH 9P41EB015871-26A1, 5R01EY017656-02, 5R01 NS051320, 4R44EB012415-02, NSF CBET-0939511, the Singapore-MIT Alliance 2, the MIT SkolTech initiative, the Hamamatsu Corp. and the Koch Institute for ...