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Tumor heterogeneity assessed by sequencing and fluorescence in situ hybridization (FISH) data [article]

Haoyun Lei, E. Michael Gertz, Alejandro A. Schäffer, Xuecong Fu, Yifeng Tao, Kerstin Heselmeyer-Haddad, Irianna Torres, Xulian Shi, Kui Wu, Guibo Li, Liqin Xu, Yong Hou (+3 others)
<span title="2020-03-01">2020</span> <i title="Cold Spring Harbor Laboratory"> bioRxiv </i> &nbsp; <span class="release-stage" >pre-print</span>
In the present work, we develop computational methods to combine sequencing with multiplex interphase fluorescence in situ hybridization (miFISH) to exploit the complementary advantages of each technology  ...  SV) events in tumor evolution, which are difficult to profile accurately by prevailing sequencing methods in such a way that subsequent reconstruction by phylogenetic inference algorithms is accurate.  ...  Acknowledgements This research was supported in part by the Intramural Research Program of the National Institutes of Health, National Library of Medicine and both Center for Cancer Research and Division  ... 
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1101/2020.02.29.970392">doi:10.1101/2020.02.29.970392</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/fkxfbqvdkncvbac2fp7l4pnoe4">fatcat:fkxfbqvdkncvbac2fp7l4pnoe4</a> </span>
<a target="_blank" rel="noopener" href="https://web.archive.org/web/20200307071601/https://www.biorxiv.org/content/biorxiv/early/2020/03/01/2020.02.29.970392.full.pdf" title="fulltext PDF download" data-goatcounter-click="serp-fulltext" data-goatcounter-title="serp-fulltext"> <button class="ui simple right pointing dropdown compact black labeled icon button serp-button"> <i class="icon ia-icon"></i> Web Archive [PDF] </button> </a> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1101/2020.02.29.970392"> <button class="ui left aligned compact blue labeled icon button serp-button"> <i class="external alternate icon"></i> biorxiv.org </button> </a>

Highly Multiplexed FISH for in Situ Genomics

Maristela L. Onozato, Clarence Yapp, Douglas Richardson, Tilak Sundaresan, Varun Chahal, Jesse Lee, James P. Sullivan, Marisa W. Madden, Hyo Sup Shim, Mathew Liebers, Quan Ho, Shyamala Maheswaran (+6 others)
<span title="2019-03-09">2019</span> <i title="Elsevier BV"> <a target="_blank" rel="noopener" href="https://fatcat.wiki/container/mj3bzt4f4rdenm2p4fih7aw2c4" style="color: black;">Journal of Molecular Diagnostics</a> </i> &nbsp;
DNA fluorescence in situ hybridization is the gold standard method to detect copy number alterations, but it is limited by the number of genes one can quantify simultaneously.  ...  The quantification of changes in gene copy number is critical to our understanding of tumor biology and for the clinical management of cancer patients.  ...  Supplemental Data Supplemental material for this article can be found at https://doi.org/10.1016/j.jmoldx.2019.01.010.  ... 
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1016/j.jmoldx.2019.01.010">doi:10.1016/j.jmoldx.2019.01.010</a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pubmed/30862547">pmid:30862547</a> <a target="_blank" rel="external noopener" href="https://pubmed.ncbi.nlm.nih.gov/PMC6504678/">pmcid:PMC6504678</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/m3wxtiaxenhxxg2hxwegqsdmku">fatcat:m3wxtiaxenhxxg2hxwegqsdmku</a> </span>
<a target="_blank" rel="noopener" href="https://web.archive.org/web/20200505153902/http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC6504678&amp;blobtype=pdf" title="fulltext PDF download" data-goatcounter-click="serp-fulltext" data-goatcounter-title="serp-fulltext"> <button class="ui simple right pointing dropdown compact black labeled icon button serp-button"> <i class="icon ia-icon"></i> Web Archive [PDF] <div class="menu fulltext-thumbnail"> <img src="https://blobs.fatcat.wiki/thumbnail/pdf/f0/96/f096f3bb8ec0573629c1607ea41ececfb98eea18.180px.jpg" alt="fulltext thumbnail" loading="lazy"> </div> </button> </a> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1016/j.jmoldx.2019.01.010"> <button class="ui left aligned compact blue labeled icon button serp-button"> <i class="external alternate icon"></i> elsevier.com </button> </a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6504678" title="pubmed link"> <button class="ui compact blue labeled icon button serp-button"> <i class="file alternate outline icon"></i> pubmed.gov </button> </a>

Oncogene Amplification Detection by Fluorescence In Situ Hybridization (FISH)

Akishi Ooi
<span title="">2001</span> <i title="Japan Society of Histochemistry &amp; Cytochemistry"> <a target="_blank" rel="noopener" href="https://fatcat.wiki/container/vg5h6r2y2nflzkyrzszbfgq3w4" style="color: black;">Acta Histochemica et Cytochemica</a> </i> &nbsp;
Fluorescence in situ hybridization (FISH) represents the newest methodological approach for testing for genetic alterations in individual cells.  ...  A direct correlation can be made with the morphology, so that tissue localization of such genetic events as tumor heterogeneity and tumor progression is feasible.  ...  Fluorescence in situ hybridization (FISH), a powerful tool for genetic evaluation, permits the microscopic identification and localization of genetic aberrations not only in metaphase chromosomes but also  ... 
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1267/ahc.34.391">doi:10.1267/ahc.34.391</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/644jus7e2jf6dmpfjdaf74emam">fatcat:644jus7e2jf6dmpfjdaf74emam</a> </span>
<a target="_blank" rel="noopener" href="https://web.archive.org/web/20170924095536/https://www.jstage.jst.go.jp/article/ahc/34/6/34_6_391/_pdf" title="fulltext PDF download" data-goatcounter-click="serp-fulltext" data-goatcounter-title="serp-fulltext"> <button class="ui simple right pointing dropdown compact black labeled icon button serp-button"> <i class="icon ia-icon"></i> Web Archive [PDF] <div class="menu fulltext-thumbnail"> <img src="https://blobs.fatcat.wiki/thumbnail/pdf/70/16/7016ccfe19ca0f629906be64a63212e37585d78b.180px.jpg" alt="fulltext thumbnail" loading="lazy"> </div> </button> </a> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1267/ahc.34.391"> <button class="ui left aligned compact blue labeled icon button serp-button"> <i class="unlock alternate icon" style="background-color: #fb971f;"></i> Publisher / doi.org </button> </a>

Application of Fluorescence In Situ Hybridization (FISH) Technique for the Detection of Genetic Aberration in Medical Science

Zubair Ahmed Ratan, Sojib Bin Zaman, Varshil Mehta, Mohammad Faisal Haidere, Nusrat Jahan Runa, Nasrin Akter
<span title="2017-06-09">2017</span> <i title="Cureus, Inc."> <a target="_blank" rel="noopener" href="https://fatcat.wiki/container/74mctp3frjfttdv6dx4dzh6lbm" style="color: black;">Cureus</a> </i> &nbsp;
MDM2 fluorescence in situ hybridization provided excellent data for distinguishing the diseases [40] .  ...  T-FISH for specific transcripts in cells lymphocytic tissue type on paraffin embedded tissue sections by using fluorescence in situ hybridization.  ... 
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.7759/cureus.1325">doi:10.7759/cureus.1325</a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pubmed/28690958">pmid:28690958</a> <a target="_blank" rel="external noopener" href="https://pubmed.ncbi.nlm.nih.gov/PMC5501716/">pmcid:PMC5501716</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/3orzmrlc7rbd5nhrsaohmn6smy">fatcat:3orzmrlc7rbd5nhrsaohmn6smy</a> </span>
<a target="_blank" rel="noopener" href="https://web.archive.org/web/20190427102252/https://assets.cureus.com/uploads/review_article/pdf/7529/1538591157-20181003-4-neoxpc.pdf" title="fulltext PDF download" data-goatcounter-click="serp-fulltext" data-goatcounter-title="serp-fulltext"> <button class="ui simple right pointing dropdown compact black labeled icon button serp-button"> <i class="icon ia-icon"></i> Web Archive [PDF] <div class="menu fulltext-thumbnail"> <img src="https://blobs.fatcat.wiki/thumbnail/pdf/d7/e5/d7e55ed25176b60ea5a192f97318f73be5dfa184.180px.jpg" alt="fulltext thumbnail" loading="lazy"> </div> </button> </a> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.7759/cureus.1325"> <button class="ui left aligned compact blue labeled icon button serp-button"> <i class="unlock alternate icon" style="background-color: #fb971f;"></i> Publisher / doi.org </button> </a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5501716" title="pubmed link"> <button class="ui compact blue labeled icon button serp-button"> <i class="file alternate outline icon"></i> pubmed.gov </button> </a>

Bright-Field HER2 Dual In Situ Hybridization (DISH) Assay vs Fluorescence In Situ Hybridization (FISH)

Faye F. Gao, David J. Dabbs, Kristine L. Cooper, Rohit Bhargava
<span title="2014-01-01">2014</span> <i title="Oxford University Press (OUP)"> <a target="_blank" rel="noopener" href="https://fatcat.wiki/container/bszuz2yhevhp5cayjkl6mzjxpe" style="color: black;">American Journal of Clinical Pathology</a> </i> &nbsp;
Objectives: To compare the INFORM HER2 bright-field dual in situ hybridization (DISH) DNA probe cocktail assay with the PathVysion fluorescence in situ hybridization (FISH) assay on 103 invasive breast  ...  In addition, 20 known IHC 3+ breast carcinomas analyzed by DISH showed clusters of the HER2 gene consistent with unequivocal amplification.  ...  of American Pathologists; DISH, dual in situ hybridization; FISH, fluorescence in situ hybridization.  ... 
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1309/ajcp6cxs8osrhxir">doi:10.1309/ajcp6cxs8osrhxir</a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pubmed/24343743">pmid:24343743</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/iwnkw4ssgrgo7jevw4oy4siuwi">fatcat:iwnkw4ssgrgo7jevw4oy4siuwi</a> </span>
<a target="_blank" rel="noopener" href="https://web.archive.org/web/20180727044011/https://watermark.silverchair.com/ajcpath141-0102.pdf?token=AQECAHi208BE49Ooan9kkhW_Ercy7Dm3ZL_9Cf3qfKAc485ysgAAAbAwggGsBgkqhkiG9w0BBwagggGdMIIBmQIBADCCAZIGCSqGSIb3DQEHATAeBglghkgBZQMEAS4wEQQMVH15UqdsFWNJsrxDAgEQgIIBY1UAgYua1TrjfNZi_xew8fAE8WNoVGNIPkruC0FvQORPMjbx9QYpxD4v7hCtiU6o95hqt1RuCIhaa6eaoZgOVwIXlk-XJCcgmkQsnkHxVa4yhVBvCUzxSGi_-Z40azCHAfHcBa-CTPeL4VN5G6MOJcBcPg4xFfbtdTIWSX7iOOMVJrTW3DftHLMJEBhlH_6luY5qiU9QXc_pTLR4NpZk3ZDJcFfCKwLhyrxXVbvTS2aJbYjpnuIAUCUQi3gIA72N-2EVfsqBpATDaB3d_L78w3Bp3eNfDtdEIb0GbphttRSD1EYMTRWfuAGYtYFb4RPh2tI3SFB48TzgSozqJZelAUxk2I0h_df3-x7AbmFMhR7rGKfQcg_NrlxrHKoQkA5ru5AUfO0VZlKPjOfZx2fGhZ7sF037sMQrLJECBdIHJsUNvwMP7jdfcli4_p231XV3wgcbdMugmk-TBVB3HeR2WXi8-TY" title="fulltext PDF download" data-goatcounter-click="serp-fulltext" data-goatcounter-title="serp-fulltext"> <button class="ui simple right pointing dropdown compact black labeled icon button serp-button"> <i class="icon ia-icon"></i> Web Archive [PDF] <div class="menu fulltext-thumbnail"> <img src="https://blobs.fatcat.wiki/thumbnail/pdf/41/9b/419b2990ca704d2bc88c15b3d07872fe5b90558b.180px.jpg" alt="fulltext thumbnail" loading="lazy"> </div> </button> </a> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1309/ajcp6cxs8osrhxir"> <button class="ui left aligned compact blue labeled icon button serp-button"> <i class="external alternate icon"></i> Publisher / doi.org </button> </a>

FISH and chips: a review of microfluidic platforms for FISH analysis

Pablo Rodriguez-Mateos, Nuno Filipe Azevedo, Carina Almeida, Nicole Pamme
<span title="2020-01-21">2020</span> <i title="Springer Science and Business Media LLC"> <a target="_blank" rel="noopener" href="https://fatcat.wiki/container/ollh3evfzjflzb5vxt6wwibeam" style="color: black;">Medical Microbiology and Immmunology</a> </i> &nbsp;
Fluorescence in situ hybridization (FISH) allows visualization of specific nucleic acid sequences within an intact cell or a tissue section.  ...  It is based on molecular recognition between a fluorescently labeled probe that penetrates the cell membrane of a fixed but intact sample and hybridizes to a nucleic acid sequence of interest within the  ...  visualization, coining the term fluorescence in situ hybridization (FISH) [3] .  ... 
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1007/s00430-019-00654-1">doi:10.1007/s00430-019-00654-1</a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pubmed/31965296">pmid:31965296</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/nwwmuqbswfc7vkgri33bschrfm">fatcat:nwwmuqbswfc7vkgri33bschrfm</a> </span>
<a target="_blank" rel="noopener" href="https://web.archive.org/web/20200710120239/http://repositorium.sdum.uminho.pt/bitstream/1822/65540/1/document_53431_1.pdf" title="fulltext PDF download" data-goatcounter-click="serp-fulltext" data-goatcounter-title="serp-fulltext"> <button class="ui simple right pointing dropdown compact black labeled icon button serp-button"> <i class="icon ia-icon"></i> Web Archive [PDF] <div class="menu fulltext-thumbnail"> <img src="https://blobs.fatcat.wiki/thumbnail/pdf/0e/bb/0ebbd49391e94c2285e2ef12ed3bdf8717ed4421.180px.jpg" alt="fulltext thumbnail" loading="lazy"> </div> </button> </a> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1007/s00430-019-00654-1"> <button class="ui left aligned compact blue labeled icon button serp-button"> <i class="external alternate icon"></i> springer.com </button> </a>

EGFRMutation Is a Better Predictor of Response to Tyrosine Kinase Inhibitors in Non–Small Cell Lung Carcinoma Than FISH, CISH, and Immunohistochemistry

Lynette M. Sholl, Yun Xiao, Victoria Joshi, Beow Y. Yeap, Leigh-Anne Cioffredi, David M. Jackman, Charles Lee, Pasi A. Jänne, Neal I. Lindeman
<span title="">2010</span> <i title="Oxford University Press (OUP)"> <a target="_blank" rel="noopener" href="https://fatcat.wiki/container/bszuz2yhevhp5cayjkl6mzjxpe" style="color: black;">American Journal of Clinical Pathology</a> </i> &nbsp;
Fluorescence in situ hybridization (FISH), chromogenic in situ hybridization (CISH), immunohistochemical analysis, and DNA sequencing were compared in this study of 40 NSCLC samples from TKI-treated patients  ...  Keywords EGFR; Non-small cell lung carcinoma; Mutation; Fluorescence in situ hybridization; Chromogenic in situ hybridization; Immunohistochemistry; Erlotinib A subset of non-small cell lung carcinomas  ...  Acknowledgments Supported by grant R01 CA114465 from National Institutes of Health, Bethesda, MD (Dr Jänne).  ... 
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1309/ajcpst1cthzs3psz">doi:10.1309/ajcpst1cthzs3psz</a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pubmed/20472851">pmid:20472851</a> <a target="_blank" rel="external noopener" href="https://pubmed.ncbi.nlm.nih.gov/PMC3156055/">pmcid:PMC3156055</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/nemfbaljfra5rkv536naenqmja">fatcat:nemfbaljfra5rkv536naenqmja</a> </span>
<a target="_blank" rel="noopener" href="https://web.archive.org/web/20180719154527/https://dash.harvard.edu/bitstream/handle/1/32705642/nihms299356.pdf?sequence=1" title="fulltext PDF download" data-goatcounter-click="serp-fulltext" data-goatcounter-title="serp-fulltext"> <button class="ui simple right pointing dropdown compact black labeled icon button serp-button"> <i class="icon ia-icon"></i> Web Archive [PDF] <div class="menu fulltext-thumbnail"> <img src="https://blobs.fatcat.wiki/thumbnail/pdf/aa/e8/aae82750eab5b1afb60b4447f7929fbed54dec9e.180px.jpg" alt="fulltext thumbnail" loading="lazy"> </div> </button> </a> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1309/ajcpst1cthzs3psz"> <button class="ui left aligned compact blue labeled icon button serp-button"> <i class="external alternate icon"></i> Publisher / doi.org </button> </a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3156055" title="pubmed link"> <button class="ui compact blue labeled icon button serp-button"> <i class="file alternate outline icon"></i> pubmed.gov </button> </a>

A curated collection of tissue microarray images and clinical outcome data of prostate cancer patients

Qing Zhong, Tiannan Guo, Markus Rechsteiner, Jan H. Rüschoff, Niels Rupp, Christian Fankhauser, Karim Saba, Ashkan Mortezavi, Cédric Poyet, Thomas Hermanns, Yi Zhu, Holger Moch (+2 others)
<span title="2017-03-14">2017</span> <i title="Springer Nature"> <a target="_blank" rel="noopener" href="https://fatcat.wiki/container/a577b42d4nfhddl7kurxnuyzem" style="color: black;">Scientific Data</a> </i> &nbsp;
Design Type(s) disease state design • cell type comparison design Measurement Type(s) Gene Deletion • cell image data Technology Type(s) chromosome organization assay by fluorescence in situ hybridization  ...  Here we describe a high-resolution tissue microarray (TMA) image dataset from a cohort of 71 prostate tissue samples, which was hybridized with bright-field dual colour chromogenic and silver in situ hybridization  ...  Our ISHProfiler supports broad applications in biomedical and computational research and alleviates the limitations of the gold standard method, fluorescence in situ hybridization (FISH) 7 .  ... 
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1038/sdata.2017.14">doi:10.1038/sdata.2017.14</a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pubmed/28291248">pmid:28291248</a> <a target="_blank" rel="external noopener" href="https://pubmed.ncbi.nlm.nih.gov/PMC5349242/">pmcid:PMC5349242</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/pinwb32yurhifb2cmlf74gdsvq">fatcat:pinwb32yurhifb2cmlf74gdsvq</a> </span>
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Responses to crizotinib in patients with ALK-positive lung adenocarcinoma who tested immunohistochemistry (IHC)-positive and fluorescence in situ hybridization (FISH)-negative

Di Ma, Zheng Wang, Lin Yang, Xinlin Mu, Yan Wang, Xinming Zhao, Junling Li, Dongmei Lin
<span title="2016-07-13">2016</span> <i title="Impact Journals, LLC"> <a target="_blank" rel="noopener" href="https://fatcat.wiki/container/yubgl6cdcrekxpjzhshpw23l3i" style="color: black;">OncoTarget</a> </i> &nbsp;
in situ hybridization (FISH) is still the "gold-standard" method recommended by the US National Comprehensive Cancer Network (NCCN) guideline for NSCLC.  ...  In addition, they were also tested by FISH, qRT-PCR, next-generation sequencing (NGS), and RNAscope ISH analysis.  ...  Table 2 : 2 Pathologic characteristics and molecular test results in the 6 patients ALK FISH, % of split signals by FISH; EGFR, epidermal growth factor receptor; FISH, fluorescence in situ hybridization  ... 
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.18632/oncotarget.10560">doi:10.18632/oncotarget.10560</a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pubmed/27418132">pmid:27418132</a> <a target="_blank" rel="external noopener" href="https://pubmed.ncbi.nlm.nih.gov/PMC5325453/">pmcid:PMC5325453</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/jcfgm3446zgg5ewxpaxvwss2wm">fatcat:jcfgm3446zgg5ewxpaxvwss2wm</a> </span>
<a target="_blank" rel="noopener" href="https://web.archive.org/web/20180722154649/http://www.oncotarget.com/index.php?journal=oncotarget&amp;page=article&amp;op=download&amp;path%5B%5D=10560&amp;path%5B%5D=33370" title="fulltext PDF download" data-goatcounter-click="serp-fulltext" data-goatcounter-title="serp-fulltext"> <button class="ui simple right pointing dropdown compact black labeled icon button serp-button"> <i class="icon ia-icon"></i> Web Archive [PDF] </button> </a> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.18632/oncotarget.10560"> <button class="ui left aligned compact blue labeled icon button serp-button"> <i class="unlock alternate icon" style="background-color: #fb971f;"></i> Publisher / doi.org </button> </a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5325453" title="pubmed link"> <button class="ui compact blue labeled icon button serp-button"> <i class="file alternate outline icon"></i> pubmed.gov </button> </a>

A CAR RNA FISH assay reveals functional and spatial heterogeneity of chimeric antigen receptor T cells in tissue [article]

Karsten Eichholz, Alvason Zhenzua Li, Kurt Diem, Semih U Tareen, Mike Jensen, Jia Zhu, Lawrence Corey
<span title="2020-08-24">2020</span> <i title="Cold Spring Harbor Laboratory"> bioRxiv </i> &nbsp; <span class="release-stage" >pre-print</span>
In situ, CAR T cell exhibited a heterogenous effector gene expression and this was related to the distance from tumor cells, allowing a quantitative assessment of the potential in vivo effectiveness.  ...  The platform offers the potential to study immune functions engineered cells in situ with their target cells in tissues with high statistical power and thus, as an important tool for preclinical and potentially  ...  the RNAscope Multiplex Fluorescent Assay version 2 with the 157 fluorophores Opal 570 and Opal 650 (PerkinElmer) according the manufacturer's protocol for 158 multiplex fluorescent in situ hybridization  ... 
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1101/2020.08.21.260935">doi:10.1101/2020.08.21.260935</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/5konlnmxuvef7frbwo4r4qzlwq">fatcat:5konlnmxuvef7frbwo4r4qzlwq</a> </span>
<a target="_blank" rel="noopener" href="https://web.archive.org/web/20210718023251/https://www.biorxiv.org/content/biorxiv/early/2021/01/16/2020.08.21.260935.full.pdf" title="fulltext PDF download" data-goatcounter-click="serp-fulltext" data-goatcounter-title="serp-fulltext"> <button class="ui simple right pointing dropdown compact black labeled icon button serp-button"> <i class="icon ia-icon"></i> Web Archive [PDF] <div class="menu fulltext-thumbnail"> <img src="https://blobs.fatcat.wiki/thumbnail/pdf/ae/76/ae76cc9eab238e23290d183c5db81ad0b29522cb.180px.jpg" alt="fulltext thumbnail" loading="lazy"> </div> </button> </a> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1101/2020.08.21.260935"> <button class="ui left aligned compact blue labeled icon button serp-button"> <i class="external alternate icon"></i> biorxiv.org </button> </a>

A novel multiple FISH array for the detection of genetic aberrations in cancer

Ruiyun Li, Zhenqiu Liu, Tao Fan, Feng Jiang
<span title="2006-03-06">2006</span> <i title="Springer Nature"> <a target="_blank" rel="noopener" href="https://fatcat.wiki/container/d3f6n54x25ho7bxaem7itvedxi" style="color: black;">Laboratory Investigation</a> </i> &nbsp;
Interphase multicolor fluorescence in situ hybridization (IM-FISH) has great promise for improving cancer diagnosis because it can directly visualize multiple changes in chromosomes and gene copy number  ...  A comparison of the data with the results of cytology and commercial four-color FISH suggested that the multiple FISH array had the highest sensitivity for cancer detection.  ...  Huangyang in China for providing parts of specimens tested in the study.  ... 
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1038/labinvest.3700408">doi:10.1038/labinvest.3700408</a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pubmed/16518404">pmid:16518404</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/rzyxost2k5fijdffpuclbpyovq">fatcat:rzyxost2k5fijdffpuclbpyovq</a> </span>
<a target="_blank" rel="noopener" href="https://web.archive.org/web/20200313030536/https://www.nature.com/articles/3700408.pdf?error=cookies_not_supported&amp;code=09d13d97-304b-4af1-ba33-da648b07f78c" title="fulltext PDF download" data-goatcounter-click="serp-fulltext" data-goatcounter-title="serp-fulltext"> <button class="ui simple right pointing dropdown compact black labeled icon button serp-button"> <i class="icon ia-icon"></i> Web Archive [PDF] <div class="menu fulltext-thumbnail"> <img src="https://blobs.fatcat.wiki/thumbnail/pdf/d7/42/d742c3a04f3e1477e52bea0eb4dd40e59a3eca93.180px.jpg" alt="fulltext thumbnail" loading="lazy"> </div> </button> </a> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1038/labinvest.3700408"> <button class="ui left aligned compact blue labeled icon button serp-button"> <i class="external alternate icon"></i> Publisher / doi.org </button> </a>

DNA FISH Diagnostic Assay on Cytological Samples of Thyroid Follicular Neoplasms

Philippe Vielh, Zsofia Balogh, Voichita Suciu, Catherine Richon, Bastien Job, Guillaume Meurice, Alexander Valent, Ludovic Lacroix, Virginie Marty, Nelly Motte, Philippe Dessen, Bernard Caillou (+7 others)
<span title="2020-09-06">2020</span> <i title="MDPI AG"> <a target="_blank" rel="noopener" href="https://fatcat.wiki/container/2zwku6u6nfdcri773tpisi6ldi" style="color: black;">Cancers</a> </i> &nbsp;
be follicular neoplasms, confirmed our previous findings and showed the feasibility of the DNA FISH (DNA fluorescent in situ hybridization) assay.  ...  The second independent cohort (validation set) of 60 pts confirmed these data on touch preparations of frozen follicular neoplasms by triple DNA fluorescent in situ hybridization using selected commercially  ...  Abbreviations: aCGH: array comparative genomic hybridization; FISH: fluorescent in situ hybridization. * The case was positive for X gain by aCGH but was not available for FISH. ** Out of the eight cases  ... 
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.3390/cancers12092529">doi:10.3390/cancers12092529</a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pubmed/32899953">pmid:32899953</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/3babgx4i2bggxehksnlloxjycu">fatcat:3babgx4i2bggxehksnlloxjycu</a> </span>
<a target="_blank" rel="noopener" href="https://web.archive.org/web/20200919003452/https://res.mdpi.com/d_attachment/cancers/cancers-12-02529/article_deploy/cancers-12-02529-v2.pdf" title="fulltext PDF download" data-goatcounter-click="serp-fulltext" data-goatcounter-title="serp-fulltext"> <button class="ui simple right pointing dropdown compact black labeled icon button serp-button"> <i class="icon ia-icon"></i> Web Archive [PDF] <div class="menu fulltext-thumbnail"> <img src="https://blobs.fatcat.wiki/thumbnail/pdf/22/52/22523ff7ee583481d8c9504bbd1e3d67277f0bfb.180px.jpg" alt="fulltext thumbnail" loading="lazy"> </div> </button> </a> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.3390/cancers12092529"> <button class="ui left aligned compact blue labeled icon button serp-button"> <i class="unlock alternate icon" style="background-color: #fb971f;"></i> mdpi.com </button> </a>

Comparison of the IHC, FISH, SISH and qPCR methods for the molecular diagnosis of breast cancer

D. Tvrd�k
<span title="2012-05-17">2012</span> <i title="Spandidos Publications"> <a target="_blank" rel="noopener" href="https://fatcat.wiki/container/56fuhprbgrhcrhe3voxurfl64e" style="color: black;">Molecular Medicine Reports</a> </i> &nbsp;
In all cases, the overexpression/amplification level of Her2 was determined using manual immunohistochemistry (IHC) and/or automatic IHC, fluorescence in situ hybridization (FISH), silver in situ hybridization  ...  Moreover, in contrast to IHC or SISH/ FISH, the results obtained by qPCR were not encumbered with any subjective error on the part of the evaluator.  ...  Acknowledgements This study was supported by grant IGA MZ ČR NS/10575-3. We wish to thank Lenka Matiášková, Jana Šídlová and Blanka Véghová for their precise laboratory technician work.  ... 
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.3892/mmr.2012.919">doi:10.3892/mmr.2012.919</a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pubmed/22614884">pmid:22614884</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/gxqjicoapnaelgrbi5422eop5y">fatcat:gxqjicoapnaelgrbi5422eop5y</a> </span>
<a target="_blank" rel="noopener" href="https://web.archive.org/web/20180719054036/https://www.spandidos-publications.com/mmr/6/2/439/download" title="fulltext PDF download" data-goatcounter-click="serp-fulltext" data-goatcounter-title="serp-fulltext"> <button class="ui simple right pointing dropdown compact black labeled icon button serp-button"> <i class="icon ia-icon"></i> Web Archive [PDF] <div class="menu fulltext-thumbnail"> <img src="https://blobs.fatcat.wiki/thumbnail/pdf/cc/43/cc43dc4b78cd274d462fbebe28394de4d119b5f5.180px.jpg" alt="fulltext thumbnail" loading="lazy"> </div> </button> </a> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.3892/mmr.2012.919"> <button class="ui left aligned compact blue labeled icon button serp-button"> <i class="external alternate icon"></i> Publisher / doi.org </button> </a>

A CAR RNA FISH assay to study functional and spatial heterogeneity of chimeric antigen receptor T cells in tissue

Karsten Eichholz, Alvason Zhenhua Li, Kurt Diem, Michael Claus Jensen, Jia Zhu, Lawrence Corey
<span title="2021-06-21">2021</span> <i title="Springer Science and Business Media LLC"> <a target="_blank" rel="noopener" href="https://fatcat.wiki/container/tnqhc2x2aneavcd3gx5h7mswhm" style="color: black;">Scientific Reports</a> </i> &nbsp;
In situ, CAR T cell exhibited a heterogenous effector gene expression and this was related to the distance from tumor cells, allowing a quantitative assessment of the potential in vivo effectiveness.  ...  The platform offers the potential to study immune functions of genetically engineered cells in situ with their target cells in tissues with high statistical power and thus, can serve as an important tool  ...  This independent research was supported by National Institute of Health grant UM1 A126623 and by the Gilead Sciences HIV Cure Grant Program.  ... 
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1038/s41598-021-92196-x">doi:10.1038/s41598-021-92196-x</a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pubmed/34155235">pmid:34155235</a> <a target="_blank" rel="external noopener" href="https://pubmed.ncbi.nlm.nih.gov/PMC8217486/">pmcid:PMC8217486</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/iy3o3hd46vh2bn2rhkum236qwy">fatcat:iy3o3hd46vh2bn2rhkum236qwy</a> </span>
<a target="_blank" rel="noopener" href="https://web.archive.org/web/20210718234012/https://www.nature.com/articles/s41598-021-92196-x.pdf?error=cookies_not_supported&amp;code=8ee01c64-04a0-40e8-9112-b1b0b74e4612" title="fulltext PDF download" data-goatcounter-click="serp-fulltext" data-goatcounter-title="serp-fulltext"> <button class="ui simple right pointing dropdown compact black labeled icon button serp-button"> <i class="icon ia-icon"></i> Web Archive [PDF] <div class="menu fulltext-thumbnail"> <img src="https://blobs.fatcat.wiki/thumbnail/pdf/a9/ba/a9ba47251c3d9d5449d4de92ff048be9aa92da7e.180px.jpg" alt="fulltext thumbnail" loading="lazy"> </div> </button> </a> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1038/s41598-021-92196-x"> <button class="ui left aligned compact blue labeled icon button serp-button"> <i class="unlock alternate icon" style="background-color: #fb971f;"></i> Publisher / doi.org </button> </a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8217486" title="pubmed link"> <button class="ui compact blue labeled icon button serp-button"> <i class="file alternate outline icon"></i> pubmed.gov </button> </a>

Determination of HER-2 Status and Chromosome 17 Polysomy in Breast Carcinomas Comparing HercepTest and PathVysion FISH Assay

Dolly Varshney, Yvonne Y. Zhou, Stephen A. Geller, Randa Alsabeh
<span title="2004-01-01">2004</span> <i title="Oxford University Press (OUP)"> <a target="_blank" rel="noopener" href="https://fatcat.wiki/container/bszuz2yhevhp5cayjkl6mzjxpe" style="color: black;">American Journal of Clinical Pathology</a> </i> &nbsp;
A b s t r a c t We evaluated and compared 2 HER-2 tests (immunohistochemical analysis [HercepTest, DAKO, Carpinteria, CA] and fluorescence in situ hybridization [FISH]) and assessed chromosome 17 polysomy  ...  HER-2 status was obtained in 690 cases. The rinse step in the HercepTest before and after addition of the visualization reagent was 2 minutes in 188 cases and was increased to 5 minutes in 600 cases.  ...  and fluorescence in situ hybridization (FISH) to assess HER-2 gene amplification.  ... 
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1309/fuqh-92b0-3902-5lhg">doi:10.1309/fuqh-92b0-3902-5lhg</a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pubmed/15272532">pmid:15272532</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/yxdb7tatizcwzbi4jbtir3lfji">fatcat:yxdb7tatizcwzbi4jbtir3lfji</a> </span>
<a target="_blank" rel="noopener" href="https://web.archive.org/web/20170707035347/https://oup.silverchair-cdn.com/oup/backfile/Content_public/Journal/ajcp/121/1/10.1309/FUQH92B039025LHG/2/ajcpath121-0070.pdf?Expires=1499485005&amp;Signature=aoJQKRuZZAQQC5xfFIUZmWgzwS6E7KoKYPEs9ZepkQ6dO6f2SsH0MvEoMMD2I-LwP7z2VZIEQjuf18-7gRsOkvhdRfFLkwc2XjK7gJdvjuCtu9CLfriDYxeam~LvU0Na9acgzbcP8holCDq-PeJwuX6Cf4-AvBaxEpBofmX1IUh7xS-8inMzmxBF6zhauEYQDjNqYftJjfA3kMk00h6Utebgu06zBZgch6vLhu7SvE8w~emAuLxW7KyAj576hSTc9A4gQz0taURo4~WOYK~uBrOfKXiRJg2xB8qKI9gr7DRgmfTxSmxDZTTz7JpBHOTHwpK9NNUXcf5yIpjm9UVv5A__&amp;Key-Pair-Id=APKAIUCZBIA4LVPAVW3Q" title="fulltext PDF download" data-goatcounter-click="serp-fulltext" data-goatcounter-title="serp-fulltext"> <button class="ui simple right pointing dropdown compact black labeled icon button serp-button"> <i class="icon ia-icon"></i> Web Archive [PDF] <div class="menu fulltext-thumbnail"> <img src="https://blobs.fatcat.wiki/thumbnail/pdf/e5/a2/e5a24fb1a25a80e73df51c0b0bb703bd160f3830.180px.jpg" alt="fulltext thumbnail" loading="lazy"> </div> </button> </a> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1309/fuqh-92b0-3902-5lhg"> <button class="ui left aligned compact blue labeled icon button serp-button"> <i class="external alternate icon"></i> Publisher / doi.org </button> </a>
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