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Transmembrane Helix Dynamics of Bacterial Chemoreceptors Supports a Piston Model of Signalling

Benjamin A. Hall, Judith P. Armitage, Mark S. P. Sansom, James M. Briggs
2011 PLoS Computational Biology  
Overall, this analysis supports a swinging-piston model of transmembrane signalling by Tar and related chemoreceptors.  ...  Bacterial chemoreceptors are one of the best studied such systems, with a wealth of biophysical and mutational data indicating a key role for the TM2 helix in signalling.  ...  The outcome of this analysis supports a piston-like model of TM signalling, whilst also highlighting a potential role for helix tilt in Tar.  ... 
doi:10.1371/journal.pcbi.1002204 pmid:22028633 pmcid:PMC3197627 fatcat:avhhakg3szbcpccnkz2qyrb7bu

Both piston-like and rotational motions are present in bacterial chemoreceptor signaling

Daqi Yu, Xiaomin Ma, Yuhai Tu, Luhua Lai
2015 Scientific Reports  
The piston-like model is supported by several experiments 15-17 . Molecular dynamics (MD) simulations of transmembrane helices with different sequences also support the piston-like model 18 .  ...  Our results suggest a mechanism of coupled rotation and piston-like motion for bacterial chemoreceptor signaling.  ...  This work was supported in part by the National Natural Science Foundation of China (21173013, 91313302), the Ministry of Science and Technology of China (2015CB910300), and National Institutes of Health  ... 
doi:10.1038/srep08640 pmid:25728261 pmcid:PMC4345343 fatcat:hvf4xi7aifh4nf76vaznmij25y

Transmembrane signaling in bacterial chemoreceptors

Joseph J. Falke, Gerald L. Hazelbauer
2001 TIBS -Trends in Biochemical Sciences. Regular ed  
Multiple, independent lines of evidence indicate that, in the periplasmic and transmembrane domains, conformational signaling is a piston-type sliding of the signaling helix towards the cytoplasm.  ...  This transmembrane signaling is being investigated by novel strategies, which have revealed a remarkably subtle conformational signal carried by a signaling helix that spans the entire length of the >350  ...  Acknowledgments Support provided by NIH Grant GM40731 (to J.J.F.) and GM29963 (to G.L.H.).  ... 
doi:10.1016/s0968-0004(00)01770-9 pmid:11295559 pmcid:PMC2895674 fatcat:cxvs7c53g5hzrmsjh4formqou4

Signaling and sensory adaptation in Escherichia coli chemoreceptors: 2015 update

John S. Parkinson, Gerald L. Hazelbauer, Joseph J. Falke
2015 Trends in Microbiology  
Current evidence supports a new view of receptor signaling in which stimulus information travels within receptor molecules through shifts in the dynamic properties of adjoining structural elements rather  ...  insights into the molecular signaling mechanisms of chemoreceptor networks.  ...  We regret that the limited scope of this article precluded a more comprehensive coverage of the literature on bacterial chemoreceptors.  ... 
doi:10.1016/j.tim.2015.03.003 pmid:25834953 pmcid:PMC4417406 fatcat:cfnpalcmj5d2jkqexcsoghaxou

Architecture and signal transduction mechanism of the bacterial chemosensory array: Progress, controversies, and challenges

Joseph J Falke, Kene N Piasta
2014 Current Opinion in Structural Biology  
Here we review advances towards a molecular description of the ultrastable lattice architecture and ultrasensitive signal transduction mechanism of the chemosensory array, as well as controversies and  ...  Recent research has deepened our understanding of the ancient, conserved chemosensory array that detects small molecule attractants and repellents, and directs the chemotaxis of bacterial and archaeal  ...  Multiple, independent lines of evidence support a conserved, piston-type signaling mechanism in the transmembrane signaling module of chemoreceptors (4, 46) .  ... 
doi:10.1016/j.sbi.2014.10.001 pmid:25460272 pmcid:PMC4268382 fatcat:gynkxaeyvrh5popwh2j7qk2ou4

Mechanism of Bacterial Signal Transduction Revealed by Molecular Dynamics of Tsr Dimers and Trimers of Dimers in Lipid Vesicles

Benjamin A. Hall, Judith P. Armitage, Mark S. P. Sansom, Marco Punta
2012 PLoS Computational Biology  
Bacterial chemoreceptors provide an important model for understanding signalling processes.  ...  In the serine receptor Tsr from E. coli, a binding event in the periplasmic domain of the receptor dimer causes a shift in a single transmembrane helix of roughly 0.15 nm towards the cytoplasm.  ...  From these simulations we propose a mechanism of signalling through the HAMP domain by destabilisation of the helix interfaces through a piston motion.  ... 
doi:10.1371/journal.pcbi.1002685 pmid:23028283 pmcid:PMC3447960 fatcat:papvyfl2tffgddmdznea4jsysq

Bacterial chemoreceptors: providing enhanced features to two-component signaling

Gerald L Hazelbauer, Wing-Cheung Lai
2010 Current Opinion in Microbiology  
Chemoreceptors are central to the enhancements. These transmembrane homodimers associate in trimers and in clusters of signaling complexes containing from a few to thousands of receptors.  ...  Homodimer signaling initiates with helical piston sliding that is converted to modulation of competing packing modes of adjacent segments of an extended helical coiled coil.  ...  Acknowledgments Our laboratory is supported by GM29963 from the National Institute of Health.  ... 
doi:10.1016/j.mib.2009.12.014 pmid:20122866 pmcid:PMC2863001 fatcat:f7iiqmdlprhp5pvxxyfqwd7wdq

Bacterial chemoreceptors: high-performance signaling in networked arrays

Gerald L. Hazelbauer, Joseph J. Falke, John S. Parkinson
2008 TIBS -Trends in Biochemical Sciences. Regular ed  
Work in the authors' laboratories was supported by NIH grants GM29963 (G.L.H.), GM40731 (J.J.F.) and GM19559 (J.S.P.).  ...  Subramaniam for original versions of the components of Figure 1 and W.C. Lai for assistance with the manuscript.  ...  to be a piston-like sliding of the signaling helix towards the cytoplasm [49] .  ... 
doi:10.1016/j.tibs.2007.09.014 pmid:18165013 pmcid:PMC2890293 fatcat:u2nxszfokzeqjluuymiqstqz3y

Side Chains at the Membrane−Water Interface Modulate the Signaling State of a Transmembrane Receptor†

Aaron S. Miller, Joseph J. Falke
2004 Biochemistry  
model for transmembrane signaling in bacterial chemoreceptors.  ...  Studies of chimeric receptors indicate that broad classes of bacterial chemoreceptors may share a conserved mechanism of transmembrane signaling (13-16).  ...  Sandy Parkinson (University of Utah), Jerry Hazelbauer (University of Missouri), and other members of the Falke group.  ... 
doi:10.1021/bi0360206 pmid:14967017 pmcid:PMC2890265 fatcat:m5zz5xetdjguxcavx3cyvnv36y

Transmembrane Signaling Is Anything but Rigid

M. D. Manson
2011 Journal of Bacteriology  
My biochemist friends shudder when I use Archimedes's six basic machines to describe how bacterial chemoreceptors function during transmembrane signaling.  ...  The essential conclusion from the modeling is that the 1.4-Å piston-like inward displacement of one helix 4 of the periplasmic domain induced by aspartate binding (9, 16) is converted into a bowing of  ...  All of the recent genetic and biochemical analyses of intact chemoreceptors can be organized into a unified model for transmembrane signal transduction in which the HAMP domain acts as a dynamic bundle  ... 
doi:10.1128/jb.05874-11 pmid:21803989 pmcid:PMC3187394 fatcat:dahgm66plbh7jfkkab5wzmmy2m

In vivo cross-linking and transmembrane helix dynamics support a bidirectional non-piston model of signaling within E. coli EnvZ [article]

Rahmi Yusuf, Tuyet Linh Nguyen, Annika Heininger, Robert J. Lawrence, Benjamin A. Hall, Roger R. Draheim
2017 bioRxiv   pre-print
Taken together, these data strongly support that EnvZ employs a non-piston-type mechanism during transmembrane communication.  ...  transmembrane communication while ruling out piston-type repositioning of TM2.  ...  Mutational analyses of HAMP helices suggest a dynamic 580 bundle model of input-output signalling in chemoreceptors. Mol Microbiol 73:801-14. 23.  ... 
doi:10.1101/206888 fatcat:27q4muznyzhrti5worefgtba24

Transmembrane Signaling of Chemotaxis Receptor Tar: Insights from Molecular Dynamics Simulation Studies

Hahnbeom Park, Wonpil Im, Chaok Seok
2011 Biophysical Journal  
The piston-like movement of a transmembrane helix induced by ligand binding on the periplasmic side is transformed into a combination of both longitudinal and transversal movements of the helix on the  ...  The current results are consistent with the previously suggested dynamic bundle model in which the HAMP dynamics change is a key to the signaling.  ...  About a decade ago, a computational study on transmembrane signaling of chemoreceptors was reported using a simple modeling scheme and limited molecular dynamics (MD) simulations (20) .  ... 
doi:10.1016/j.bpj.2011.05.030 pmid:21689529 pmcid:PMC3123975 fatcat:qko5s7cv3re57hk7iljh27hike

The archaeal sensory rhodopsin II/transducer complex: a model for transmembrane signal transfer

Johann P Klare, Valentin I Gordeliy, Jörg Labahn, Georg Büldt, Heinz-Jürgen Steinhoff, Martin Engelhard
2004 FEBS Letters  
Electron paramagnetic resonance data and the atomic resolution structure of the receptor molecule in complex with the transmembrane segment of its cognate transducer provided a model for signal transfer  ...  This mechanism might also be relevant for eubacterial chemoreceptor signalling.  ...  In accordance with the dimeric organisation of the eubacterial chemoreceptors the transducer crystallises as a dimer with the four transmembrane helices arranged as a four-helix bundle.  ... 
doi:10.1016/s0014-5793(04)00193-0 pmid:15111099 fatcat:3ovi7ddsfvb2ve2op7ywjojxmy

Signaling and Adaptation Modulate the Dynamics of the Photosensoric Complex of Natronomonas pharaonis

Philipp S. Orekhov, Daniel Klose, Armen Y. Mulkidjanian, Konstantin V. Shaitan, Martin Engelhard, Johann P. Klare, Heinz-Jürgen Steinhoff, Nir Ben-Tal
2015 PLoS Computational Biology  
Overall these observations suggest a signaling mechanism based on dynamic allostery resembling models previously proposed for E. coli chemoreceptors, indicating similar properties of signal transduction  ...  for archaeal photoreceptors and bacterial chemoreceptors.  ...  Acknowledgments We thank the Supercomputing Center of Moscow University for computational resources.  ... 
doi:10.1371/journal.pcbi.1004561 pmid:26496122 pmcid:PMC4651059 fatcat:gldjoythlfhxxcqfropktom3va

Evidence for a Helix-Clutch Mechanism of Transmembrane Signaling in a Bacterial Chemoreceptor

Peter Ames, Samuel Hunter, John S. Parkinson
2016 Journal of Molecular Biology  
This helix-clutch model offers a new perspective on the mechanism of transmembrane signaling in chemoreceptors.  ...  Graphical Abstract * Corresponding author: mailing address: Keywords bacterial chemotaxis; piston model; control cable; HAMP domain; dynamic-bundle model Sensory adaptation plays a critical role in the  ...  Acknowledgements This work was supported by research grant GM19559 from the National Institute of General Medical Sciences.  ... 
doi:10.1016/j.jmb.2016.03.017 pmid:27019297 pmcid:PMC5023463 fatcat:p62dvunvljhbphgyguqp2lvc3a
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