Filters








2,728 Hits in 5.5 sec

Comparative dynamics of microglial and glioma cell motility at the infiltrative margin of brain tumours

Joseph Juliano, Orlando Gil, Andrea Hawkins-Daarud, Sonal Noticewala, Russell C. Rockne, Jill Gallaher, Susan Christine Massey, Peter A. Sims, Alexander R. A. Anderson, Kristin R. Swanson, Peter Canoll
2018 Journal of the Royal Society Interface  
growth factor-B (PDGFB)-driven rat model of glioma, in which glioma cells and microglia were each labelled with one of two different fluorescent markers.  ...  We used mathematical techniques to analyse glioma cells and microglia motility with both single cell tracking and particle image velocimetry (PIV).  ...  We gratefully acknowledge the support of the James S. McDonnell Foundation, the Ivy Foundation, the Mayo Clinic and the NIH (R01 NS060752, R01 CA164371, U54 CA210180, U54 CA143970, U54 CA193489)  ... 
doi:10.1098/rsif.2017.0582 pmid:29445035 fatcat:g4blyan73rfkxdyi37ajqhggjm

The Molecular and Phenotypic Basis of the Glioma Invasive Perivascular Niche

2017 International Journal of Molecular Sciences  
Ultimately, this culminates in glioma repopulation and migration at distances beyond the original tumour site, which is a considerable obstacle for effective treatment.  ...  Such therapeutic difficulties exist, in part, due to several glioma survival adaptations and mechanisms, which allow glioma cells to repurpose paracrine signalling pathways and ion channels within discreet  ...  It is noteworthy to observe that in addition to the major role of BK in glioma cell migration around the perivascular space, it can promote the migration of glioma cells into the surrounding brain matrix  ... 
doi:10.3390/ijms18112342 pmid:29113105 pmcid:PMC5713311 fatcat:imvmoi6as5c2xjhrkk5ca4bmbq

Mechanisms of invasion and motility of high-grade gliomas in the brain

Devin B Mair, Heather M Ames, Rong Li
2018 Molecular Biology of the Cell  
In this review, we discuss the mechanisms of glioma cell migration and how they may be targeted in anti-invasion therapies.  ...  Cell migration involves the sensing of a migratory cue, followed by polarization in the direction of the cue, and reorganization of the actin cytoskeleton to allow for a protrusive leading edge and a contractile  ...  ACKNOWLEDGMENTS We thank Hung-Ji Tsai for assisting in manuscript revisions.  ... 
doi:10.1091/mbc.e18-02-0123 pmid:30325290 pmcid:PMC6254577 fatcat:cuclbazvwne4jo2kdaquyba3q4

Automated time-lapse microscopy and high-resolution tracking of cell migration

Joseph S. Fotos, Vivek P. Patel, Norman J. Karin, Murali K. Temburni, John T. Koh, Deni S. Galileo
2006 Cytotechnology (Dordrecht)  
We describe a novel fully automated high-throughput time-lapse microscopy system and evaluate its performance for precisely tracking the motility of several glioma and osteoblastic cell lines.  ...  To illustrate one application, fluorescently labeled tumor cells were seeded onto cell monolayers expressing ectopic adhesion molecules, and this resulted in consistently reduced tumor cell migration velocities  ...  Patel were awarded undergraduate research fellowships through grants to the Univ. of Delaware from the Howard Hughes Medical Institute and the Ronald E. McNair Program. We gratefully thank Dr.  ... 
doi:10.1007/s10616-006-9006-7 pmid:19002890 pmcid:PMC3449480 fatcat:c6rxpepxwjes7nm7xmevrtdcvm

The biology and mathematical modelling of glioma invasion: a review

J. C. L. Alfonso, K. Talkenberger, M. Seifert, B. Klink, A. Hawkins-Daarud, K. R. Swanson, H. Hatzikirou, A. Deutsch
2017 Journal of the Royal Society Interface  
Indeed, the widespread infiltration of healthy brain tissue by glioma cells is largely responsible for poor prognosis and the difficulty of finding curative therapies.  ...  In this review, we start with a brief introduction to current biological knowledge about glioma invasion, and then critically review and highlight future challenges for mathematical models of glioma invasion  ...  Glioma cells migrate along blood vessels by using the outward vessel-parenchyma interface and the lumen of the perivascular space [76] .  ... 
doi:10.1098/rsif.2017.0490 pmid:29118112 pmcid:PMC5721156 fatcat:etlbfsh5p5cxxk2pkhb3xzospe

Predicting confined 1D cell migration from parameters calibrated to a 2D motor-clutch model

Louis S. Prahl, Maria R. Stanslaski, Pablo Vargas, Matthieu Piel, David J. Odde
2020 Biophysical Journal  
These spaces restrict cell motion to a single-spatial dimension (1D), a feature that is not captured in traditional in vitro cell migration assays performed on flat, unconfined two-dimensional (2D) substrates  ...  These results are consistent with glioma cells utilizing a motor-clutch system to migrate in confined environments.  ...  Lennon-Dum enil for use of laboratory equipment and the Bioimaging Cell and Tissue Core Facility at Institut Curie for use of microscopes during initial data collection.  ... 
doi:10.1016/j.bpj.2020.01.048 pmid:32145191 pmcid:PMC7136340 fatcat:nhvdlbfeffb2biojyrlc2bifdq

Recapitulating in vivo-like plasticity of glioma cell invasion along blood vessels and in astrocyte-rich stroma

Pavlo Gritsenko, William Leenders, Peter Friedl
2017 Histochemistry and Cell Biology  
Each 3D assay recapitulated distinct aspects of major glioma invasion patterns identified in mouse xenografts and patient brain samples, including individually migrating cells, collective strands extending  ...  In conjunction, these organotypic assays enable a range of invasion modes used by glioma cells and will be applicable for mechanistic analysis and targeting of glioma cell dissemination.  ...  To mimic in vivo-like glioma cell invasion in vitro, we here applied three tissue-inspired 3D environments combining multicellular glioma spheroids and reconstituted microanatomic features of vascular  ... 
doi:10.1007/s00418-017-1604-2 pmid:28825130 pmcid:PMC5602046 fatcat:njci6apw7fhvdaiabg63bxwyse

Translation of the ecological trap concept to glioma therapy: the cancer cell trap concept

Boudewijn van der Sanden, Florence Appaix, François Berger, Laurent Selek, Jean-Paul Issartel, Didier Wion
2013 Future Oncology  
It exploits and diverts the invasive potential of glioma cells to guide their migration towards specific locations where a local therapy can be delivered efficiently.  ...  The presence of such a trap can drive a local population to extinction. The cancer cell trap concept is the translation of the ecological trap into glioma therapy.  ...  Experimental procedures for tracking the migration of implanted cells in the rodent brain are available.  ... 
doi:10.2217/fon.13.30 pmid:23718302 pmcid:PMC4788699 fatcat:cegsk6ghhvaytm4pmbi3llhslm

A vascular perspective on neuronal migration

Marta Segarra, Bettina C. Kirchmaier, Amparo Acker-Palmer
2015 Mechanisms of Development  
This migration mode allows neuroblast navigation in physiological and also pathological conditions, such as neuronal precursor migration after ischemic stroke or cerebral invasion of glioma tumor cells  ...  In adulthood, long distance neuronal migration occurs in a glial-independent manner since radial glia cells differentiate into astrocytes after birth.  ...  Acknowledgements The authors wish to thank the Deutsche Forschungsgemeinschaft (AC180/2-1, AC180/2-2, CRC834, CRC1080, Clusters of Excellence EXC 115 and EXC147), the Gutenberg Research College (GRC) at  ... 
doi:10.1016/j.mod.2015.07.004 pmid:26192337 fatcat:bnrbe2hpwndhdbnd6nzomtwgom

Direct inhibition of myosin II effectively blocks glioma invasion in the presence of multiple motogens

Sanja Ivkovic, Christopher Beadle, Sonal Noticewala, Susan C. Massey, Kristin R. Swanson, Laura N. Toro, Anne R. Bresnick, Peter Canoll, Steven S. Rosenfeld, Yu-Li Wang
2012 Molecular Biology of the Cell  
First, malignant glioma cells are stimulated to invade brain through the activation of multiple signaling cascades Supplemental Material can be found at: that are not accounted for in simple in vitro assays  ...  Second, even though there is a high degree of redundancy in pro-migratory signaling cascades in gliomas, blocking tumor invasion by directly targeting myosin II remains effective.  ...  Cell Lines and Cell Quantitative analysis of glioma cell migration: Individual cell tracks from time-lapse microscopy were plotted to a common origin to generate Wind-Rose plots to spatially compare the  ... 
doi:10.1091/mbc.e11-01-0039 pmid:22219380 pmcid:PMC3279383 fatcat:kdcynuaojbfwtgbbmnmxw2kpsi

The Role of Myosin II in Glioma Invasion of the Brain

Christopher Beadle, Marcela C. Assanah, Pascale Monzo, Richard Vallee, Steven S. Rosenfeld, Peter Canoll, Erika Holzbaur
2008 Molecular Biology of the Cell  
The ability of gliomas to invade the brain limits the efficacy of standard therapies. In this study, we have examined glioma migration in living brain tissue by using two novel in vivo model systems.  ...  In contrast, on a two-dimensional surface, glioma cells migrate more like fibroblasts, and they do not require myosin II to move.  ...  This work was supported in part by NIH grant NS045070 (P.C.) and NIH grant HDO 40182 (RV).  ... 
doi:10.1091/mbc.e08-03-0319 pmid:18495866 pmcid:PMC2488307 fatcat:wqnp3vqnnjh3tfoc4dqnk2bxru

Glioma cell migration in confined microchannels via a motor-clutch mechanism [article]

Louis S. Prahl, Maria R. Stanslaski, Pablo Vargas, Matthieu Piel, David J. Odde
2018 bioRxiv   pre-print
To study glioma cell migration in an in vitro system that reproduces some of these features, we used microfluidic devices with 60 μm2 cross-sectional area channels that confine cells into one-dimensional  ...  Our results outline a method for testing biophysical mechanisms of tumor cell migration in confined spaces and predicting the effects of anti-motility therapy.  ...  Acknowledgements Thanks to members of the Odde and Piel laboratories for helpful discussions, and thanks to Prof. Margaret Titus for comments on the manuscript. The authors thank Dr.  ... 
doi:10.1101/500843 fatcat:q3xk6uycwvanzbmn2ywgfn4nzq

The role of myosin II in glioma invasion: A mathematical model

Wanho Lee, Sookkyung Lim, Yangjin Kim, Joseph Najbauer
2017 PLoS ONE  
Glioma cells migrate through a dense network of normal cells in microenvironment and spread long distances within brain.  ...  This suggests that the migration of glioma cells may be regulated by specific substrates and structures in brain.  ...  Obviously, migration of glioma cells in the perivascular space of Virchow-Robin (which is essentially fluid-filled space) [105, 106] is different from migration along white matter tracts in the neuropil  ... 
doi:10.1371/journal.pone.0171312 pmid:28166231 pmcid:PMC5293275 fatcat:ckb4znoq65fgld6qx7iplunz24

Visualization of spatiotemporal dynamics of human glioma stem cell invasion

Ryota Tamura, Hiroyuki Miyoshi, Oltea Sampetrean, Munehisa Shinozaki, Yukina Morimoto, Chizuru Iwasawa, Raita Fukaya, Yutaka Mine, Hirotaka Masuda, Tetsuo Maruyama, Minoru Narita, Hideyuki Saya (+3 others)
2019 Molecular Brain  
These characteristics of GSC invasion shared histological features observed in glioblastoma patients.  ...  Glioblastoma exhibits phenotypic and genetic heterogeneity, aggressive invasiveness, therapeutic resistance, and tumor recurrence, which can be explained by the existence of glioma stem cells (GSCs).  ...  Funding This work was supported in part by grants from the Japan Society for the Promotion of Science (JSPS) (  ... 
doi:10.1186/s13041-019-0462-3 pmid:31060588 pmcid:PMC6503361 fatcat:ina6mug42nemflpifo2fidk23m

Glial progenitor cell recruitment drives aggressive glioma growth: mathematical and experimental modelling

S. C. Massey, M. C. Assanah, K. A. Lopez, P. Canoll, K. R. Swanson
2012 Journal of the Royal Society Interface  
provide a novel mechanism to explain the variability in the rates of proliferation and dispersion observed in human gliomas.  ...  a mechanism for progenitor cell recruitment, wherein paracrine PDGF signalling stimulates migration and proliferation of progenitors derived from the local brain environment.  ...  These results suggest that differences in the degree to which glioma cells stimulate migration and proliferation of nonneoplastic glia in the surrounding brain tissue can explain the differences in glioma  ... 
doi:10.1098/rsif.2012.0030 pmid:22319102 pmcid:PMC3385768 fatcat:rcupklmcdngern4kem4uly7uqm
« Previous Showing results 1 — 15 out of 2,728 results