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Paradoxical embolism (PDE) refers to direct passage of venous thrombi into the arterial circulation through an arteriovenous shunt. Case 1 presented with initial symptoms of shock and cerebral infarction. Case 2 developed middle cerebral artery occlusion during angiography. 2 cases were diagnosed as PDE. They received thrombolytic therapy and anticoagulant therapy. The patients had recovery. This report highlights the myriad clinical manifestations of PDE and underlines the importance ofdoi:10.1097/md.0000000000007332 pmid:28658147 pmcid:PMC5500069 fatcat:chsykfauazgubpnmdatw5tdbra
more »... ous history taking and physical examination for early diagnosis.
Reactive oxygen species (ROS) play important regulatory roles in plant growth and development, as well as in cell differentiation and stress responses. Respiratory burst oxidase homolog (RBOH) is the key enzyme in ROS production. So far, the Rboh family genes in Pyropia yezoensis have not been comprehensively characterized, and whether their function was involved in the formation of archeospores is still unknown. In this study, a total of 11 PyRboh genes were identified from the P. yezoensisdoi:10.3389/fpls.2022.929299 pmid:35903222 pmcid:PMC9322803 doaj:456d64b277b847698582287105564712 fatcat:fgfg73wxw5fbfkeq7tr4me4oem
more »... ome by homology mining. Through phylogenetic analysis, it is suggested that the PyRboh genes were evolutionarily conserved among the lineages of red algae, but a few genes exhibited a species-specific manner. The treatment of P. yezoensis blades with NADPH oxidase inhibitor diphenylene iodonium (DPI) could significantly inhibit the formation of archeospores, suggesting that RBOH may be involved in the formation of archeospores. According to PyRboh gene expression analysis using the P. yezoensis strains with obvious differences in releasing archeospores, it is showed that the expression trends of most genes were consistent, with no significant difference between strains, whereas the expression pattern of the two P. yezoensis-specific genes (PyRbohJ and PyRbohK) was positively correlated with the amount of archeospores. Furthermore, as treatment of blades with allantoin resulted in a significant increase in the release of archeospores, the expression levels of PyRbohJ and PyRbohK were also consistently upregulated, further confirming the relationship between the two genes and archeospore formation. These findings provide insights into the molecular mechanism of P. yezoensis archeospore formation.
Hong et al. developed a droplet-based microfluidics to conduct drug screening in cells from cancer cell lines or primary tumors. ...doi:10.1186/s12951-021-01045-6 pmid:34635104 pmcid:PMC8507141 fatcat:ipqfdmrw4ngqxfy7zabcd6a25e
2) (河北科技师范学院物理系, 秦皇岛 066004)doi:10.7498/aps.64.077103 fatcat:upuohy5puvfxbpuias3viqiodi
The gene encoding the GroEL chaperonin is duplicated in nearly 30% of bacterial genomes; and although duplicated groEL genes have been comprehensively determined to have distinct physiological functions in different species, the mechanisms involved have not been characterized to date. Myxococcus xanthus DK1622 has two copies of the groEL gene, each of which can be deleted without affecting cell viability; however, the deletion of either gene does result in distinct defects in the cellulardoi:10.1371/journal.pgen.1003306 pmid:23437010 pmcid:PMC3578752 fatcat:kx4573ct4zdurhuemb4y76ukke
more »... hock response, predation, and development. In this study, we show that, from the expression levels of different groELs, the distinct functions of groEL1 and groEL2 in predation and development are probably the result of the substrate selectivity of the paralogous GroEL chaperonins, whereas the lethal effect of heat shock due to the deletion of groEL1 is caused by a decrease in the total groEL expression level. Following a bioinformatics analysis of the composition characteristics of GroELs from different bacteria, we performed region-swapping assays in M. xanthus, demonstrating that the differences in the apical and the C-terminal equatorial regions determine the substrate specificity of the two GroELs. Site-directed mutagenesis experiments indicated that the GGM repeat sequence at the C-terminus of GroEL1 plays an important role in functional divergence. Divergent functions of duplicated GroELs, which have similar patterns of variation in different bacterial species, have thus evolved mainly via alteration of the apical and the C-terminal equatorial regions. We identified the specific substrates of strain DK1622's GroEL1 and GroEL2 using immunoprecipitation and mass spectrometry techniques. Although 68 proteins bound to both GroEL1 and GroEL2, 83 and 46 proteins bound exclusively to GroEL1 or GroEL2, respectively. The GroEL-specific substrates exhibited distinct molecular sizes and secondary structures, providing an encouraging indication for GroEL evolution for functional divergence.
Gonadotropins have been widely used in human-assisted reproduction and animal science for the past four decades. However, the effects of gonadotropins on oocyte maturation at the molecular and biochemical levels are poorly understood. To determine the effects of gonadotropins (recombinant follicle stimulating hormone and urinary human menopausal gonadotropin) on oocyte maturation, we used the bovine oocyte in vitro maturation model. First, we studied the effects of increasing gonadotropindoi:10.1095/biolreprod.114.117945 pmid:24943039 fatcat:fulwddqf3baotgpqd6tuysfzr4
more »... trations on nuclear maturation and mitochondrial function in oocytes. Gonadotropins at concentrations of 0.075 and 0.75 IU/ml improved nuclear maturation and increased inner mitochondrial membrane potential and ATP levels; however, there were no beneficial effects at concentrations of 7.5 and 75 IU/ml. Second, we studied the effects of increasing gonadotropin concentrations on the status of methylation in matured (MII) oocytes. Aberrant methylation and demethylation of H19, SNRPN, and PEG3 genes were observed in MII oocytes at all concentrations except 0.075 IU/ml. The expression of genes that function in spindle formation, cell cycle control, and methylation was also downregulated by high gonadotropin concentrations. In conclusion, we established the optimal gonadotropin concentration (i.e., 0.075 IU/ml) to be used for bovine oocyte in vitro maturation studies. These results may provide a guide for clinical stimulation protocols and help to reduce the risks associated with gonadotropin administration during in vitro fertilization treatment. gonadotropin, methylation, mitochondria, oocyte FIG. 3. The effect of increasing concentrations of gonadotropins on the ATP level in individual oocytes. Data are presented as mean 6 SEM. Different letters indicate statistically significant differences (P , 0.05 or P , 0.01).
Tian et al.Cytologia 76(4) ...doi:10.1508/cytologia.76.411 fatcat:subgaqcrp5gphhn7ehvrkhzrje
Aquaporin (AQP)-dependent cell migration has broad implications in angiogenesis, tumor metastasis, wound healing, glial scarring and other events requiring cell movement. There are 13 isoforms of AQP (0-12) that have been identified in mammals. It is unclear whether AQP5 plays a role in the development of endometrial cancer. We recently demonstrated that ovarian steroids may affect the expression of AQP5 in the female genital tract. In this study, we considered whether AQP5 may affect celldoi:10.3892/ol.2012.738 pmid:22844365 pmcid:PMC3402746 fatcat:uwj6zbx7ujbylg2fjvoh4met5i
more »... tion in Ishikawa cells, an adenocarcinoma cell line derived from the endometrium. The results showed that the downregulation of AQP5 results in reduced Ishikawa cell migration. The estrogen (E2) receptor in the promoter of AQP5 mediated the regulation of AQP5 expression in the normal endometrium and endometrial cancer. By contrast, the upregulation of AQP5 by E2 increased cell migration, invasion and adhesion through increased annexin-2, which is responsible for F-actin remodeling and rearrangement. E2 regulates Ishikawa cell migration by regulating the AQP5 expression.
Author Details Hong-Hong Yan, born in 1979, is currently an associate professor at Taiyuan University of Science and Technology, China. ...doi:10.1186/s10033-018-0284-8 fatcat:6d3mzih2yjhy7mxvcko6eag33a
Verificationof "Channel-ProbabilityModel" ofGrainYieldEstimation ZHENGHong-yan 1 ,LIJing-ya 1 ,LIUShu-tian 1 ,HUANGZhi-ping 1 ,MIChang-hong 1 ,HOUYan-lin 1* ,WANGNong 1 ,CAIYan-ming 1 , WANGShuo-jin 2 ... ZHENGHong-yan,LIJing-ya,LIUShu-tian,etal.Verificationof"Channel-ProbabilityModel"ofGrainYieldEstimation[J]. JournalofAgricultural ResourcesandEnvironment,2016,33 (4) :390-396. ...doi:10.13254/j.jare.2015.0194 doaj:7a570923f4af4ed29de432489aa1669a fatcat:wbfzuxikxzgwpngi63vwlctf2a
The present study aimed to investigate the role and underlying mechanisms of microRNA16 (miR-16) on proliferation, apoptosis and invasion of glioma cells. The cell models of miR-16 upregulation and Negative control group (NC group) were built. The cell functions of different groups were detected by colony formation assay, transwell chamber assay, proliferation, apoptosis and cycle experiments. The intracranial orthotopic transplantation animal models were built to different groups: miR-16doi:10.18632/oncotarget.18510 pmid:28903382 pmcid:PMC5589621 fatcat:ex7mk5na4zd6no67hymjkvakpe
more »... group, miR-16 antagomir group and their NC group. The expressions of miR-16, Wip1, ATM and p53 were measured by qRT-PCR, western blot and immunohistochemistry. As a result, miR-16 overexpressed groups had lower cloning formation rate and proliferation rate, less invasive cells, higher early apoptosis rate than the control groups. G1 phase was significantly smaller compared miR-16 overexpressed groups with the control groups, and S phase significantly lesser. Cell growth was retardated. Differences were statistically significant (P <0.05). Compared with miR-16 overexpressed groups and NC groups, the Wip1 gene and protein expression were downregulated, while ATM and p53 genes, p-ATM and p-p53 proteins were upregulated. The differences were statistically significant (P <0.05). Taken together, our findings demonstrated that miR-16 suppressed glioma cell proliferation and invasion, promoted apoptosis and inhibited cell cycle by targeting Wip1-ATM-p53 signaling pathway.
Conflict of interest Fan Yang, Shan Jiang, Xiao-ning He, Hong-chao Li, Hong-yan Wu, Tian-tian Zhang and Jing Wu have no conflicts of interest. ...doi:10.1007/s41669-020-00212-z pmid:32285402 fatcat:gyzw3ejh6jh7pmlvfuqx6eqzcm
Acknowledgements We thank Miss Yuexian Yan and doctors: Wei Shi, Jijun Li, Yan Song, Zhixiang Ren, Xiyao Tian and Rengang Wu for their cooperation in the investigation of psychological rating scales. ...doi:10.1016/s1561-5413(09)60028-4 fatcat:gg4lm4jhr5f47fwmssp5kqexfm
A pilot study on the ethanol extracts of Agrimonia pilosa found to have anti-α-glucosidase and anti-inflammatory activities. Subsequent chemical study afforded a new phenylethyl isocoumarin glycoside (1) and eight known compounds (2-9). The structure of 1 was elucidated by comprehensive spectroscopic analysis and chemical transformations. All compounds showed modest α-glucosidase inhibitory activity (IC50 values ranging from 36.8 to 210.7 μM), which was lower than that of the positive controldoi:10.6084/m9.figshare.12854864.v1 fatcat:ug3ocsct3zgg3k7bl7cwtsbx4e
more »... arbose (IC50=301.9 μM). Those compounds except inactive compounds 3 and 6 showed weak anti-inflammatory activity.
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