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For the quantification of cell features from images (image cytometry), computer-based algorithms are favoured to avoid bias introduced by the subjective perception of the observer. ... These particles bear interesting properties for biomedical applications and, for several years, have been in the focus of research. ... The authors are thankful to Christian Allebach for proofreading the manuscript. ...doi:10.17192/z2015.0375 fatcat:jl52emwzxjfctldqcrei3xjfyy