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Genetic structure and function of an early transcript of visna virus

V Mazarin, I Gourdou, G Quérat, N Sauze, R Vigne
1988 Journal of Virology  
the 5' and 3' ends, respectively, of the env gene region.  ...  They were translated in vitro and gave rise to three proteins, two of 19 and 17 kilodaltons, termed VEP1, and one of 16.5 kilodaltons, termed STM.  ...  V.M. and I.G. were, respectively, Fellows of the Ministere de la Recherche et de l'Enseignement Superieur and of the Ligue Nationale de Lutte contre le Cancer. LITERATURE CITED  ... 
doi:10.1128/jvi.62.12.4813-4818.1988 fatcat:34577la5qjhevkk22taarxo7ti

Genetic structure and function of an early transcript of visna virus

V Mazarin, I Gourdou, G Quérat, N Sauze, R Vigne
1988 Journal of Virology  
the 5' and 3' ends, respectively, of the env gene region.  ...  They were translated in vitro and gave rise to three proteins, two of 19 and 17 kilodaltons, termed VEP1, and one of 16.5 kilodaltons, termed STM.  ...  V.M. and I.G. were, respectively, Fellows of the Ministere de la Recherche et de l'Enseignement Superieur and of the Ligue Nationale de Lutte contre le Cancer. LITERATURE CITED  ... 
pmid:2846892 pmcid:PMC253606 fatcat:jrb2jddtsvgphnh7truez5sghm

Molecular evolution of human and rabbit beta-globin mRNAs

F. C. Kafatos, A. Efstratiadis, B. G. Forget, S. M. Weissman
1977 Proceedings of the National Academy of Sciences of the United States of America  
that not all silent mutations in globin mRNA are neutral.  ...  The primary structures of human and rabbit fl-globin mRNAs are compared.  ...  Excluding three deletions in the 5' noncoding region and a highly divergent part of the 3' noncoding region (star in Fig. 1 ), a total of 66 substitutions in 553 sites is observed (11.9%).  ... 
doi:10.1073/pnas.74.12.5618 pmid:271989 pmcid:PMC431834 fatcat:jky5fym6czbkvj4nn5lw2exb6m

Germiston virus transcriptase requires active 40S ribosomal subunits and utilizes capped cellular RNAs

P Vialat, M Bouloy
1992 Journal of Virology  
Moreover, we showed that the RNA transcripts synthesized in a transcription-translation reaction were capped and that most of them have acquired the 5' terminal sequences of the alpha- or beta-globin mRNA  ...  The transcriptase associated with Germiston virus was assayed in an in vitro reaction in which transcription was coupled to translation by adding reticulocyte lysate under the appropriate salt conditions  ...  Munns for his generous gift of anti-cap antiserum, M. Kozak for kindly providing edeine, and D. Kolakofsky for helpful discussions. This work was supported in part by grant 861003 from INSERM.  ... 
pmid:1731108 pmcid:PMC240767 fatcat:xqblnabc2jedzo6h2wjiugokxi

Identification of a recently evolved goat embryonic beta-globin pseudogene which retains transcriptional activity in vitro

S G Shapiro, J B Lingrel
1984 Molecular and Cellular Biology  
I-epsilon II-psi beta X-beta C-epsilon III-epsilon IV-psi beta Z-beta A-epsilon V-3', was isolated, and the sequence of the gene was determined. epsilon V is most homologous to the first gene in each  ...  Homology of epsilon V to epsilon I is very high (93.2%) in coding regions, and all transcription, processing, and potential translation consensus sequence elements appear to be present, although the Hogness  ...  We also are indebted to Donal Luse for providing valuable advice and discussion concerning in vitro transcription. We thank Mary C. Fitzgerald for her excellent technical assistance.  ... 
doi:10.1128/mcb.4.10.2120 pmid:6095041 pmcid:PMC369030 fatcat:btukvej5kna6nesfzbvsowkc4y

Both the 5' untranslated region and the sequences surrounding the start site contribute to efficient initiation of translation in vitro

D Falcone, D W Andrews
1991 Molecular and Cellular Biology  
The role of RNA sequences in the 5' leader region between the cap site and initiating AUG in mediating translation was examined in vitro.  ...  In contrast to earlier suggestions that increased leader efficiency results from higher affinity of the leader for a limiting factor, our experiments suggest that increased translation from the beta-globin  ...  The pattern of synthesis shown is essentially the same as in Fig. 4A ; therefore, the increase in translational efficiency due to the UTK leader is essentially independent of template RNA concentration  ... 
doi:10.1128/mcb.11.5.2656 pmid:2017171 pmcid:PMC360035 fatcat:ltcfl2hhifbu3nwxqed7g5jtse

Rapid mRNA degradation mediated by the c-fos 3' AU-rich element and that mediated by the granulocyte-macrophage colony-stimulating factor 3' AU-rich element occur through similar polysome-associated mechanisms

E Winstall, M Gamache, V Raymond
1995 Molecular and Cellular Biology  
The iron-responsive element was introduced in the 5' untranslated regions of alpha-globin mRNAs that harbored in their 3' untranslated regions either the c-fos ARE or the granulocyte-macrophage colony-stimulating  ...  The different 3' noncoding AU-rich elements (ARE) that mediate the degradation of many short-lived mRNAs may function through distinct decay pathways; translation-dependent and -independent mechanisms  ...  This work was supported by a grant from the Medical Research Council of Canada (MRC) and was initiated in the Laboratoire de Neurogénétique Moléculaire du Centre de Recherche Université Laval-Robert Giffard  ... 
doi:10.1128/mcb.15.7.3796 pmid:7540719 pmcid:PMC230618 fatcat:p42cirmec5dn3ouna6vtpvhqvq

Control of human -globin mRNA stability and its impact on beta-thalassemia phenotype

I. Peixeiro, A. L. Silva, L. Romao
2011 Haematologica  
tion of aberrant b-globin mRNAs. In addition, we emphasize the importance of these pathways in modulating the severity of the b-thalassemia phenotype.  ...  When general rules for NMD are not obeyed: example of the nonsense b-globin transcripts The human b-globin gene has two intervening noncoding sequences (introns) and three coding regions (exons) that are  ...  indices. 75 In contrast, if the PTC is located further downstream at exon 3 of the b-globin mRNA, for example at codon 121 (GAA→TAA) or 127 (CAG→TAG), 88 NMD is also not triggered.  ... 
doi:10.3324/haematol.2010.039206 pmid:21357703 pmcid:PMC3105653 fatcat:zhcyqockeffmzl2f6b3fh2sbtm

Two distinct and frequently mutated regions of retinoblastoma protein are required for binding to SV40 T antigen

S Huang, N P Wang, B Y Tseng, W H Lee, E H Lee
1990 EMBO Journal  
A 5- to 10-fold increase in translational efficiency in the reticulocyte lysate was obtained when the 5' non-coding region of RB mRNA was replaced with that of beta-globin mRNA or a plant viral RNA, alfalfa  ...  Thus the regions of RB defined in vitro as necessary for interaction with T might be physiologically relevant as well, and might play a fundamental role in normal RB protein function.  ...  ., and from the National Eye Institute (EY 05758) to W.-H.L. S.H. is a recipient of a postdoctoral fellowship from the Cancer Research Coordinating Committee, University of California.  ... 
pmid:2189724 pmcid:PMC551886 fatcat:uxzqc2ufgncqdcr2vyv6x3dfby

Coding elements in exons 2 and 3 target c-myc mRNA downregulation during myogenic differentiation

N M Yeilding, W M Lee
1997 Molecular and Cellular Biology  
for myc mRNA downregulation, suggesting that some feature of beta-globin mRNA may potentiate the regulatory properties of myc exons 2 and 3.  ...  A similar regulatory function is not shared by all mRNAs because fusion of either myc exon 2 or myc exon 3 to CAT mRNA did not confer downregulation onto the chimeric mRNA, but fusion of the two elements  ...  We also thank Eric Russell for critical reading of the manuscript and Muhammad Rehman for technical assistance.  ... 
doi:10.1128/mcb.17.5.2698 pmid:9111340 pmcid:PMC232120 fatcat:yedmzhnhebcc5bpg322vaecjgy

Targeted Cleavage and Polyadenylation of RNA by CRISPR-Cas13 [article]

Kelly M Anderson, Pornthida Poosala, Sean R Lindley, Douglas M Anderson
2019 bioRxiv   pre-print
Alternative cleavage and polyadenylation is an important mechanism for the control of gene expression and defects in 3-prime end processing can give rise to myriad human diseases.  ...  Post-transcriptional cleavage and polyadenylation of messenger and long noncoding RNAs is coordinated by a supercomplex of ~20 individual proteins within the eukaryotic nucleus.  ...  Polyadenylation plays an essential role in controlling RNA transcript stability, nuclear export, and translation efficiency 3-6 .  ... 
doi:10.1101/531111 fatcat:pd2tuu2djvef7osef7nsxwkqiy

Bach1 Repression of Ferritin and Thioredoxin Reductase1 Is Heme-sensitive in Cells and in Vitro and Coordinates Expression with Heme Oxygenase1, beta-Globin, and NADP(H) Quinone (Oxido) Reductase1

K. J. Hintze, Y. Katoh, K. Igarashi, E. C. Theil
2007 Journal of Biological Chemistry  
Ferritin gene transcription is regulated by heme as is ferritin mRNA translation, which is mediated by the well studied mRNA⅐IRE/IRP protein complex.  ...  Bach1-DNA promoter interactions in cells were confirmed in vitro with soluble, recombinant Bach1 protein and revealed a quantitative range of Bach1/DNA stabilities: ferritin L ϳ ferritin H ϳ ␤-globin,  ...  The IRE is a specific three-dimensional loop-helix-loop-helix structure (10) in the noncoding regions of the mRNAs (11) (12) (13) (14) (15) (16) .  ... 
doi:10.1074/jbc.m700254200 pmid:17901053 fatcat:daa5pxifqbfsxkx47pimkvxkuu

SARS Coronavirus nsp1 Protein Induces Template-Dependent Endonucleolytic Cleavage of mRNAs: Viral mRNAs Are Resistant to nsp1-Induced RNA Cleavage

Cheng Huang, Kumari G. Lokugamage, Janet M. Rozovics, Krishna Narayanan, Bert L. Semler, Shinji Makino, Ralph S. Baric
2011 PLoS Pathogens  
By using primarily cellfree, in vitro translation systems, the present study revealed that the nsp1 induced endonucleolytic RNA cleavage mainly near the 59 untranslated region of capped mRNA templates.  ...  entry site (IRES) region of a dicistronic RNA template, but not in those IRES elements from hepatitis C or cricket paralysis viruses.  ...  Christopher Hellen for CSFV IRES reporter plasmid pCSFV(1-442)NS', Dr. Robert S. Fujinami and Dr. Raymond Roos for TMEV cDNA plasmid pDAFL3, and Dr.  ... 
doi:10.1371/journal.ppat.1002433 pmid:22174690 pmcid:PMC3234236 fatcat:5njrocihnzdfvhurbheni3tjbi

A 32-kilodalton protein binds to AU-rich domains in the 3' untranslated regions of rapidly degraded mRNAs

E Vakalopoulou, J Schaack, T Shenk
1991 Molecular and Cellular Biology  
An AU-rich sequence present within the 3' untranslated region has been shown to mark some short-lived mRNAs for rapid degradation.  ...  Steady-state levels of beta-globin-specific nuclear RNA, including incompletely processed RNA, and cytoplasmic mRNA were reduced.  ...  The presence of free histones and ongoing translation are essential for histone mRNA degradation (30, 38) .  ... 
doi:10.1128/mcb.11.6.3355 pmid:1903842 pmcid:PMC360189 fatcat:eviof6zqubd3xbb3pq5kknju7m

Control of globin gene transcription by erythropoietin in erythroblasts from friend virus-infected mice

M C Bondurant, R N Lind, M J Koury, M E Ferguson
1985 Molecular and Cellular Biology  
In the splenic erythroblasts, the chromatin structure in the vicinity of the beta-major globin gene was analyzed with two nucleases during these transcription rate changes.  ...  By 6 h, the transcription rate of beta-globin RNA is enhanced threefold, and by 12 h, it is nearly maximal at ca. 20 times the level of control cells which received no EP.  ...  The basal level of 3-globin mRNA is very low in untreated cells.  ... 
doi:10.1128/mcb.5.4.675 pmid:3990688 pmcid:PMC366769 fatcat:qs6sqqucd5gobgbitjdqiaik4a
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